Recombinant Human YWHAE protein (His tag) is a Human Full Length protein, in the 1 to 255 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M D D R E D L V Y Q A K L A E Q A E R Y D E M V E S M K K V A G M D V E L T V E E R N L L S V A Y K N V I G A R R A S W R I I S S I E Q K E E N K G G E D K L K M I R E Y R Q M V E T E L K L I C C D I L D V L D K H L I P A A N T G E S K V F Y Y K M K G D Y H R Y L A E F A T G N D R K E A A E N S L V A Y K A A S D I A M T E L P P T H P I R L G L A L N F S V F Y Y E I L N S P D R A C R L A K A A F D D A I A E L D T L S E E S Y K D S T L I M Q L L R D N L T L W T S D M Q G D G E E Q N K E A L Q D V E D E N Q
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
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Target data
Function
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif (PubMed:35343654). Binding generally results in the modulation of the activity of the binding partner (By similarity). Positively regulates phosphorylated protein HSF1 nuclear export to the cytoplasm (PubMed:12917326). Plays a positive role in the antiviral signaling pathway upstream of TBK1 via interaction with RIGI (PubMed:37555661). Mechanistically, directs RIGI redistribution from the cytosol to mitochondrial associated membranes where it mediates MAVS-dependent innate immune signaling during viral infection (PubMed:22607805). Plays a role in proliferation inhibition and cell cycle arrest by exporting HNRNPC from the nucleus to the cytoplasm to be degraded by ubiquitination (PubMed:37599448).
Alternative names
14-3-3 protein epsilon, 14-3-3E, YWHAE
Recommended products
Recombinant Human YWHAE protein (His tag) is a Human Full Length protein, in the 1 to 255 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- P62258-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: 89% PBS, 10% Glycerol (glycerin, glycerine), 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M D D R E D L V Y Q A K L A E Q A E R Y D E M V E S M K K V A G M D V E L T V E E R N L L S V A Y K N V I G A R R A S W R I I S S I E Q K E E N K G G E D K L K M I R E Y R Q M V E T E L K L I C C D I L D V L D K H L I P A A N T G E S K V F Y Y K M K G D Y H R Y L A E F A T G N D R K E A A E N S L V A Y K A A S D I A M T E L P P T H P I R L G L A L N F S V F Y Y E I L N S P D R A C R L A K A A F D D A I A E L D T L S E E S Y K D S T L I M Q L L R D N L T L W T S D M Q G D G E E Q N K E A L Q D V E D E N Q
- Accession
- P62258
- Protein length
- Full Length
- Predicted molecular weight
- 31.3 kDa
- Amino acids
- 1 to 255
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab219215 was purified using conventional chromatography techniques.
General info
- Function
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif (PubMed:35343654). Binding generally results in the modulation of the activity of the binding partner (By similarity). Positively regulates phosphorylated protein HSF1 nuclear export to the cytoplasm (PubMed:12917326). Plays a positive role in the antiviral signaling pathway upstream of TBK1 via interaction with RIGI (PubMed:37555661). Mechanistically, directs RIGI redistribution from the cytosol to mitochondrial associated membranes where it mediates MAVS-dependent innate immune signaling during viral infection (PubMed:22607805). Plays a role in proliferation inhibition and cell cycle arrest by exporting HNRNPC from the nucleus to the cytoplasm to be degraded by ubiquitination (PubMed:37599448).
- Sequence similarities
Belongs to the 14-3-3 family.
- Post-translational modifications
(Microbial infection) Cleaved by poliovirus protease 3C, leading to disruption of the interaction with RIGI.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product was previously labelled as 14-3-3 epsilon
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The YWHAE protein also known as 14-3-3E acts as a regulatory molecule by binding to various phosphoserine-containing proteins. It plays key roles in cellular signaling and exists as a dimer. YWHAE has a molecular mass of around 29 kDa and is widely expressed in tissues including the brain heart and skeletal muscle. Its interactions often rely on its abundant presence in various cell types showing its versatility.
- Biological function summary
YWHAE protein interacts with several enzymes and receptors forming complexes that affect their functional states. It is a member of the 14-3-3 family which facilitates signal transduction by binding to phosphorylated serine/threonine residues. This protein influences processes like cell cycle control apoptosis and stress responses emphasizing its significant roles in maintaining cellular homeostasis.
- Pathways
YWHAE plays significant roles in the Akt and MAPK pathways. In the Akt pathway YWHAE partners with proteins like BAD and FOXO which are important in controlling apoptosis and cell survival. In the MAPK pathway it regulates multiple proteins including Raf and MEK impacting cell growth and differentiation. Understanding YWHAE's interactions in these pathways highlights its influence in critical signaling events.
- Associated diseases and disorders
YWHAE has been tied to schizophrenia and neurodevelopmental disorders. In schizophrenia it interacts with proteins like DISC1 affecting neuronal migration and synaptic function. In neurodevelopmental disorders alterations in YWHAE can lead to compromised signaling that disrupts normal brain function. These connections highlight the importance of YWHAE in maintaining neurological health.
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1 product image
SDS-PAGE - Recombinant Human YWHAE protein (His tag) (ab219215)
15% SDS-PAGE analysis of 3 μg ab219215.
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