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AB187469

Recombinant Human ZADH2 protein (His tag N-Terminus)

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Recombinant Human ZADH2 protein (His tag N-Terminus) is a Human Fragment protein, in the 33 to 377 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

ZADH2, PTGR3, Prostaglandin reductase 3, PRG-3, Zinc-binding alcohol dehydrogenase domain-containing protein 2

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SDS-PAGE - Recombinant Human ZADH2 protein (AB187469)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human ZADH2 protein (AB187469)

15% SDS-PAGE analysis of 3 μg ab187469.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

Q8N4Q0

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.4 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMQKLVVTRLSPNFREAVTLSRDCPVPLPGDGDLLVRNRFVGVNASDINYSAGRYDPSVKPPFDIGFEGIGEVVALGLSASARYTVGQAVAYMAPGSFAEYTVVPASIATPVPSVKPEYLTLLVSGTTAYISLKELGGLSEGKKVLVTAAAGGTGQFAMQLSKKAKCHVIGTCSSDEKSAFLKSLGCDRPINYKTEPVGTVLKQEYPEGVDVVYESVGGAMFDLAVDALATKGRLIVIGFISGYQTPTGLSPVKAGTLPAKLLKKSASVQGFFLNHYLSKYQAAMSHLLEMCVSGDLVCEVDLGDLSPEGRFTGLESIFRAVNYMYMGKNTGKIVVELPHSVNSKL","proteinLength":"Fragment","predictedMolecularWeight":"39 kDa","actualMolecularWeight":null,"aminoAcidEnd":377,"aminoAcidStart":33,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q8N4Q0","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Zinc-binding alcohol dehydrogenase domain-containing protein 2 (ZADH2) also known by the alternate name TESK1 plays a mechanical role as an enzyme within cellular metabolism. It weighs approximately 35 kDa and typically shows expression in the liver kidney and heart tissues. This protein is involved in metabolic pathways related to alcohol processing where it facilitates the conversion of aldehydes to their corresponding alcohols utilizing NAD+ as a cofactor.
Biological function summary

ZADH2 influences cellular redox balance and detoxification processes. It does not directly participate as part of a larger protein complex but interacts with enzymes that coordinate to manage oxidative stress. In addition to converting aldehydes ZADH2 activity also links to the control of lipid metabolism partially impacting lipid peroxidation which is an important aspect of preventing cellular damage and maintaining overall cellular health.

Pathways

The involvement of ZADH2 extends to the fatty acid degradation and alcohol metabolism pathways. Within these pathways ZADH2 interacts with aldehyde dehydrogenases forming a network that contributes to the conversion of toxic aldehyde byproducts into non-toxic forms. This activity is indispensable in the regulation of homeostasis and prevention of alcohol-induced cellular damage.

ZADH2 relates to conditions such as liver cirrhosis and metabolic syndrome largely due to its role in managing aldehyde levels and oxidative stress. For liver cirrhosis ZADH2's malfunctioning can lead to accumulated toxic aldehydes which contribute to cellular damage. Metabolic syndrome may involve disrupted lipid oxidation processes with ZADH2 influencing the balance of metabolic intermediates. It interacts with peroxisome proliferator-activated receptor gamma (PPARγ) which relates to lipid homeostasis and inflammation in these conditions.
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Specifications

Form

Liquid

Additional notes

ab187469 was purified using conventional chromatography techniques.

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General info

Function

Functions as 15-oxo-prostaglandin 13-reductase and acts on 15-keto-PGE1, 15-keto-PGE2, 15-keto-PGE1-alpha and 15-keto-PGE2-alpha with highest efficiency towards 15-keto-PGE2-alpha. Overexpression represses transcriptional activity of PPARG and inhibits adipocyte differentiation.

Sequence similarities

Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily.

Subcellular localisation

Peroxisome

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Product protocols

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Target data

Functions as 15-oxo-prostaglandin 13-reductase and acts on 15-keto-PGE1, 15-keto-PGE2, 15-keto-PGE1-alpha and 15-keto-PGE2-alpha with highest efficiency towards 15-keto-PGE2-alpha. Overexpression represses transcriptional activity of PPARG and inhibits adipocyte differentiation.
See full target information PTGR3
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