Recombinant Human ZADH2 protein is a Human Fragment protein, in the 33 to 377 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S M Q K L V V T R L S P N F R E A V T L S R D C P V P L P G D G D L L V R N R F V G V N A S D I N Y S A G R Y D P S V K P P F D I G F E G I G E V V A L G L S A S A R Y T V G Q A V A Y M A P G S F A E Y T V V P A S I A T P V P S V K P E Y L T L L V S G T T A Y I S L K E L G G L S E G K K V L V T A A A G G T G Q F A M Q L S K K A K C H V I G T C S S D E K S A F L K S L G C D R P I N Y K T E P V G T V L K Q E Y P E G V D V V Y E S V G G A M F D L A V D A L A T K G R L I V I G F I S G Y Q T P T G L S P V K A G T L P A K L L K K S A S V Q G F F L N H Y L S K Y Q A A M S H L L E M C V S G D L V C E V D L G D L S P E G R F T G L E S I F R A V N Y M Y M G K N T G K I V V E L P H S V N S K L
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Functions as 15-oxo-prostaglandin 13-reductase and acts on 15-keto-PGE1, 15-keto-PGE2, 15-keto-PGE1-alpha and 15-keto-PGE2-alpha with highest efficiency towards 15-keto-PGE2-alpha. Overexpression represses transcriptional activity of PPARG and inhibits adipocyte differentiation.
Alternative names
ZADH2, PTGR3, Prostaglandin reductase 3, PRG-3, Zinc-binding alcohol dehydrogenase domain-containing protein 2
Recommended products
Recombinant Human ZADH2 protein is a Human Fragment protein, in the 33 to 377 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- Q8N4Q0-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: 80% PBS, 20% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S M Q K L V V T R L S P N F R E A V T L S R D C P V P L P G D G D L L V R N R F V G V N A S D I N Y S A G R Y D P S V K P P F D I G F E G I G E V V A L G L S A S A R Y T V G Q A V A Y M A P G S F A E Y T V V P A S I A T P V P S V K P E Y L T L L V S G T T A Y I S L K E L G G L S E G K K V L V T A A A G G T G Q F A M Q L S K K A K C H V I G T C S S D E K S A F L K S L G C D R P I N Y K T E P V G T V L K Q E Y P E G V D V V Y E S V G G A M F D L A V D A L A T K G R L I V I G F I S G Y Q T P T G L S P V K A G T L P A K L L K K S A S V Q G F F L N H Y L S K Y Q A A M S H L L E M C V S G D L V C E V D L G D L S P E G R F T G L E S I F R A V N Y M Y M G K N T G K I V V E L P H S V N S K L
- Accession
- Q8N4Q0
- Protein length
- Fragment
- Predicted molecular weight
- 39 kDa
- Amino acids
- 33 to 377
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab187469 was purified using conventional chromatography techniques.
General info
- Function
Functions as 15-oxo-prostaglandin 13-reductase and acts on 15-keto-PGE1, 15-keto-PGE2, 15-keto-PGE1-alpha and 15-keto-PGE2-alpha with highest efficiency towards 15-keto-PGE2-alpha. Overexpression represses transcriptional activity of PPARG and inhibits adipocyte differentiation.
- Sequence similarities
Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily.
- Subcellular localisation
- Peroxisome
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Zinc-binding alcohol dehydrogenase domain-containing protein 2 (ZADH2) also known by the alternate name TESK1 plays a mechanical role as an enzyme within cellular metabolism. It weighs approximately 35 kDa and typically shows expression in the liver kidney and heart tissues. This protein is involved in metabolic pathways related to alcohol processing where it facilitates the conversion of aldehydes to their corresponding alcohols utilizing NAD+ as a cofactor.
- Biological function summary
ZADH2 influences cellular redox balance and detoxification processes. It does not directly participate as part of a larger protein complex but interacts with enzymes that coordinate to manage oxidative stress. In addition to converting aldehydes ZADH2 activity also links to the control of lipid metabolism partially impacting lipid peroxidation which is an important aspect of preventing cellular damage and maintaining overall cellular health.
- Pathways
The involvement of ZADH2 extends to the fatty acid degradation and alcohol metabolism pathways. Within these pathways ZADH2 interacts with aldehyde dehydrogenases forming a network that contributes to the conversion of toxic aldehyde byproducts into non-toxic forms. This activity is indispensable in the regulation of homeostasis and prevention of alcohol-induced cellular damage.
- Associated diseases and disorders
ZADH2 relates to conditions such as liver cirrhosis and metabolic syndrome largely due to its role in managing aldehyde levels and oxidative stress. For liver cirrhosis ZADH2's malfunctioning can lead to accumulated toxic aldehydes which contribute to cellular damage. Metabolic syndrome may involve disrupted lipid oxidation processes with ZADH2 influencing the balance of metabolic intermediates. It interacts with peroxisome proliferator-activated receptor gamma (PPARγ) which relates to lipid homeostasis and inflammation in these conditions.
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1 product image
SDS-PAGE - Recombinant Human ZADH2 protein (ab187469)
15% SDS-PAGE analysis of 3 μg ab187469.
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