Recombinant human ZAK protein
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Recombinant human ZAK protein is a Human Full Length protein, expressed in Baculovirus infected Sf9 cells, with >70%, suitable for SDS-PAGE, WB, FuncS.
View Alternative Names
MLK7, MLTK, ZAK, HCCS4, MAP3K20, Mitogen-activated protein kinase kinase kinase 20, Human cervical cancer suppressor gene 4 protein, Leucine zipper- and sterile alpha motif-containing kinase, MLK-like mitogen-activated protein triple kinase, Mitogen-activated protein kinase kinase kinase MLT, Mixed lineage kinase 7, Mixed lineage kinase-related kinase, Sterile alpha motif- and leucine zipper-containing kinase AZK, HCCS-4, MLK-related kinase, MRK
- FuncS
Unknown
Functional Studies - Recombinant human ZAK protein (AB89852)
Kinase Assay demonstrating specific activity of ab89852
- FuncS
Unknown
Functional Studies - Recombinant human ZAK protein (AB89852)
The specific activity of ZAK (ab89852) was determined to be 19.8 nmol/min/mg as per activity assay protocol and was equivalent to 60 nmol/min/mg as per radiometric assay
- SDS-PAGE
Unknown
SDS-PAGE - Recombinant human ZAK protein (AB89852)
SDS-PAGE showing ab89852 at approximately 82kDa
- SDS-PAGE
Unknown
SDS-PAGE - Recombinant human ZAK protein (AB89852)
SDS PAGE analysis of ab89852
Complementary Products
Reagents, Controls & Accessories
AB116029
Prestained Protein Ladder - Extra broad molecular weight (5 - 245 kDa)
- 5
- 1
Reactivity data
Product details
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
ZAK initiates and participates in the mitogen-activated protein kinase (MAPK) signaling cascade which is integral to cell growth differentiation and apoptosis. ZAK forms part of a signaling complex that transmits signals from the membrane to the cytoplasm. Its activation leads to phosphorylation of other kinases therefore propagating the signal within the cell. Through its role in these signaling pathways ZAK influences cellular processes that maintain homeostasis in response to changing environments.
Pathways
The protein ZAK is deeply involved in the stress-activated protein kinase (SAPK) and JUN amino-terminal kinases (JNK) pathways. These pathways play significant roles in the cell's response to stress signals such as cytokines and environmental stressors. ZAK interacts with and phosphorylates other MAP kinase family members further integrating it into these pathways. Through such interactions ZAK affects related proteins like JNK1 and JNK2 which are essential in mediating stress responses at a cellular level.
Specifications
Form
Liquid
Additional notes
Affinity purified.
General info
Function
Stress-activated component of a protein kinase signal transduction cascade that promotes programmed cell death in response to various stress, such as ribosomal stress, osmotic shock and ionizing radiation (PubMed : 10924358, PubMed : 11836244, PubMed : 12220515, PubMed : 14521931, PubMed : 15350844, PubMed : 15737997, PubMed : 18331592, PubMed : 20559024, PubMed : 26999302, PubMed : 32289254, PubMed : 32610081, PubMed : 35857590). Acts by catalyzing phosphorylation of MAP kinase kinases, leading to activation of the JNK (MAPK8/JNK1, MAPK9/JNK2 and/or MAPK10/JNK3) and MAP kinase p38 (MAPK11, MAPK12, MAPK13 and/or MAPK14) pathways (PubMed : 11042189, PubMed : 11836244, PubMed : 12220515, PubMed : 14521931, PubMed : 15172994, PubMed : 15737997, PubMed : 32289254, PubMed : 32610081, PubMed : 35857590). Activates JNK through phosphorylation of MAP2K4/MKK4 and MAP2K7/MKK7, and MAP kinase p38 gamma (MAPK12) via phosphorylation of MAP2K3/MKK3 and MAP2K6/MKK6 (PubMed : 11836244, PubMed : 12220515). Involved in stress associated with adrenergic stimulation : contributes to cardiac decompensation during periods of acute cardiac stress (PubMed : 15350844, PubMed : 21224381, PubMed : 27859413). May be involved in regulation of S and G2 cell cycle checkpoint by mediating phosphorylation of CHEK2 (PubMed : 15342622).. Isoform ZAKalpha. Key component of the stress-activated protein kinase signaling cascade in response to ribotoxic stress or UV-B irradiation (PubMed : 32289254, PubMed : 32610081, PubMed : 35857590). Acts as the proximal sensor of ribosome collisions during the ribotoxic stress response (RSR) : directly binds to the ribosome by inserting its flexible C-terminus into the ribosomal intersubunit space, thereby acting as a sentinel for colliding ribosomes (PubMed : 32289254, PubMed : 32610081). Upon ribosome collisions, activates either the stress-activated protein kinase signal transduction cascade or the integrated stress response (ISR), leading to programmed cell death or cell survival, respectively (PubMed : 32610081). Dangerous levels of ribosome collisions trigger the autophosphorylation and activation of MAP3K20, which dissociates from colliding ribosomes and phosphorylates MAP kinase kinases, leading to activation of the JNK and MAP kinase p38 pathways that promote programmed cell death (PubMed : 32289254, PubMed : 32610081). Less dangerous levels of ribosome collisions trigger the integrated stress response (ISR) : MAP3K20 activates EIF2AK4/GCN2 independently of its protein-kinase activity, promoting EIF2AK4/GCN2-mediated phosphorylation of EIF2S1/eIF-2-alpha (PubMed : 32610081). Also part of the stress-activated protein kinase signaling cascade triggering the NLRP1 inflammasome in response to UV-B irradiation : ribosome collisions activate MAP3K20, which directly phosphorylates NLRP1, leading to activation of the NLRP1 inflammasome and subsequent pyroptosis (PubMed : 35857590). NLRP1 is also phosphorylated by MAP kinase p38 downstream of MAP3K20 (PubMed : 35857590). Also acts as a histone kinase by phosphorylating histone H3 at 'Ser-28' (H3S28ph) (PubMed : 15684425).. Isoform ZAKbeta. Isoform that lacks the C-terminal region that mediates ribosome-binding : does not act as a sensor of ribosome collisions in response to ribotoxic stress (PubMed : 32289254, PubMed : 32610081, PubMed : 35857590). May act as an antagonist of isoform ZAKalpha : interacts with isoform ZAKalpha, leading to decrease the expression of isoform ZAKalpha (PubMed : 27859413).
Sequence similarities
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
Post-translational modifications
Activated by phosphorylation by PKN1, followed by autophosphorylation on Thr-161 and Ser-165 (PubMed:12220515, PubMed:12761180, PubMed:15342622, PubMed:26999302). Autophosphorylation in response to ribotoxic stress promotes dissociation from colliding ribosomes and activation (PubMed:32289254, PubMed:32610081).
Target data
Product promise
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