JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB127488

Recombinant Human ZC3H14 protein (His-DHFR tag N-Terminus)

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant Human ZC3H14 protein (His-DHFR tag N-Terminus) is a Human Fragment protein, in the 128 to 306 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE.

View Alternative Names

Zinc finger CCCH domain-containing protein 14, Mammalian suppressor of tau pathology-2, Renal carcinoma antigen NY-REN-37, MSUT-2, ZC3H14

Key facts

Purity

>85% SDS-PAGE

Expression system

Escherichia coli

Tags

His-DHFR tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q6PJT7

Animal free

No

Carrier free

No

Species

Human

Reconstitution

Reconstitute in water

Storage buffer

Constituents: 0.58% Sodium chloride, 0.32% Tris HCl

storage-buffer

style
left-column

Complementary Products

Primary Antibodies

AB232936

Anti-ZC3H14 antibody

Immunoprecipitation - Anti-ZC3H14 antibody (AB232936)

  • 0
  • 0
Proteins & Peptides

AB204146

Recombinant human PIM3 protein

Functional Studies - Recombinant human PIM3 protein (AB204146)

  • 0
  • 0
Assay Kits

AB267631

Human HGFA ELISA Kit

Sandwich ELISA - Human HGFA ELISA Kit (AB267631)

  • 0
  • 0
Proteins & Peptides

AB132365

Recombinant Human THRAP5 protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human THRAP5 protein (GST tag N-Terminus) (AB132365)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column
style
left-column

Sequence info

[{"sequence":"","proteinLength":"Fragment","predictedMolecularWeight":"20.6 kDa","actualMolecularWeight":null,"aminoAcidEnd":306,"aminoAcidStart":128,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q6PJT7","tags":[{"tag":"His-DHFR","terminus":"N-Terminus"}]}]
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
False
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The zinc finger CCCH domain-containing protein ZC3H14 also known as N4BP3 or DFP1 functions as a RNA-binding protein and weighs approximately 95 kDa. This protein is expressed in several tissues with higher levels observed in the brain. It performs a critical role in post-transcriptional regulation by binding to polyadenylated RNA. ZC3H14 might directly influence mRNA stability and decay which are key components of gene expression regulation.
Biological function summary

Beyond its RNA-binding capacity ZC3H14 makes a significant impact on neuronal function as it associates with particular RNA processing activities. Though not formally part of a complex it has interactions that suggest it collaborates with other proteins in the cell to perform its functions. Its activity is particularly important in maintaining proper levels of gene expression in neurons which is critical for their health and function.

Pathways

The work of ZC3H14 in post-transcriptional regulation affects important processes such as mRNA surveillance and RNA turnover pathways. These pathways play important roles in ensuring that aberrant mRNA transcripts do not accumulate in cells. Within these pathways ZC3H14 might engage with other proteins like UPF1 which is an important factor in nonsense-mediated decay. This interaction ensures only properly processed mRNAs are translated into proteins therefore maintaining cellular homeostasis.

Abnormal function or expression of ZC3H14 links to neurological conditions notably intellectual disabilities. The mutation or loss of function of this protein leads to improper mRNA processing and neuronal dysfunction. ZC3H14 also connects to disorders of protein homeostasis due to its involvement with RNA-binding proteins that regulate gene expression in the nervous system. Understanding its precise role and interactions may provide insights into therapeutic strategies for diseases such as these.
style
left-column

Specifications

Form

Lyophilized

Additional notes

Purified via His tag

style
left-column

General info

Function

RNA-binding protein involved in the biogenesis of circular RNAs (circRNAs), which are produced by back-splicing circularization of pre-mRNAs (PubMed : 39461343). Acts by binding to both exon-intron boundary and 3'-UTR of pre-mRNAs to promote circRNA biogenesis through dimerization and the association with the spliceosome (PubMed : 39461343). Required for spermatogenesis via involvement in circRNA biogenesis (PubMed : 39461343). Regulates the pre-mRNA processing of ATP5MC1; preventing its degradation (PubMed : 27563065). Also binds the poly(A) tail of mRNAs; controlling poly(A) length in neuronal cells (PubMed : 17630287, PubMed : 24671764).

Sequence similarities

Belongs to the ZC3H14 family.

Subcellular localisation

Nucleus speckle

style
left-column

Product protocols

style
left-column

Target data

RNA-binding protein involved in the biogenesis of circular RNAs (circRNAs), which are produced by back-splicing circularization of pre-mRNAs (PubMed : 39461343). Acts by binding to both exon-intron boundary and 3'-UTR of pre-mRNAs to promote circRNA biogenesis through dimerization and the association with the spliceosome (PubMed : 39461343). Required for spermatogenesis via involvement in circRNA biogenesis (PubMed : 39461343). Regulates the pre-mRNA processing of ATP5MC1; preventing its degradation (PubMed : 27563065). Also binds the poly(A) tail of mRNAs; controlling poly(A) length in neuronal cells (PubMed : 17630287, PubMed : 24671764).
See full target information ZC3H14
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com