Recombinant Human ZCCHC3 protein is a Human Full Length protein, in the 1 to 403 aa range, expressed in Wheat germ and suitable for ELISA, WB.
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Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
Amino acid sequence
M A T G G G A E E E R K R G R P Q L L P P A R P A A R G E E A D G G R E K M G W A Q V V K N L A E K K G E F R E P R P P R R E E E S G G G G G S A G L G G P A G L A A P D L G D F P P A G R G D P K G R R R D P A G E A V D P R K K K G A A E A G R R K K A E A A A A A M A T P A R P G E A E D A A E R P L Q D E P A A A A G P G K G R F L V R I C F Q G D E G A C P T R D F V V G A L I L R S I G M D P S D I Y A V I Q I P G S R E F D V S F R S A E K L A L F L R V Y E E K R E Q E D C W E N F V V L G R S K S S L K T L F I L F R N E T V D V E D I V T W L K R H C D V L A V P V K V T D R F G I W T G E Y K C E I E L R Q G E G G V R H L P G A F F L G A E R G Y S W Y K G Q P K T C F K C G S R T H M S G S C T Q D R C F R C G E E G H L S P Y C R K G I V C N L C G K R G H A F A Q C P K A V H N S V A A Q L T G V A G H
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Nucleic acid-binding protein involved in innate immune response to DNA and RNA viruses (PubMed:30135424, PubMed:30193849). Binds DNA and RNA in the cytoplasm and acts by promoting recognition of viral nucleic acids by virus sensors, such as RIGI, IFIH1/MDA5 and CGAS (PubMed:30135424, PubMed:30193849). Acts as a co-sensor for recognition of double-stranded DNA (dsDNA) by cGAS in the cytoplasm, thereby playing a role in innate immune response to cytosolic dsDNA and DNA virus (PubMed:30135424). Binds dsDNA and probably acts by promoting sensing of dsDNA by CGAS, leading to enhance CGAS oligomerization and activation (PubMed:30135424). Promotes sensing of viral RNA by RIGI-like receptors proteins RIGI and IFIH1/MDA5 via two mechanisms: binds double-stranded RNA (dsRNA), enhancing the binding of RIGI and IFIH1/MDA5 to dsRNA and promotes 'Lys-63'-linked ubiquitination and subsequent activation of RIGI and IFIH1/MDA5 (PubMed:30193849).
Alternative names
C20orf99, ZCCHC3, Zinc finger CCHC domain-containing protein 3
Recommended products
Recombinant Human ZCCHC3 protein is a Human Full Length protein, in the 1 to 403 aa range, expressed in Wheat germ and suitable for ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
- Accession
- Q9NUD5-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- M A T G G G A E E E R K R G R P Q L L P P A R P A A R G E E A D G G R E K M G W A Q V V K N L A E K K G E F R E P R P P R R E E E S G G G G G S A G L G G P A G L A A P D L G D F P P A G R G D P K G R R R D P A G E A V D P R K K K G A A E A G R R K K A E A A A A A M A T P A R P G E A E D A A E R P L Q D E P A A A A G P G K G R F L V R I C F Q G D E G A C P T R D F V V G A L I L R S I G M D P S D I Y A V I Q I P G S R E F D V S F R S A E K L A L F L R V Y E E K R E Q E D C W E N F V V L G R S K S S L K T L F I L F R N E T V D V E D I V T W L K R H C D V L A V P V K V T D R F G I W T G E Y K C E I E L R Q G E G G V R H L P G A F F L G A E R G Y S W Y K G Q P K T C F K C G S R T H M S G S C T Q D R C F R C G E E G H L S P Y C R K G I V C N L C G K R G H A F A Q C P K A V H N S V A A Q L T G V A G H
- Accession
- Q9NUD5
- Protein length
- Full Length
- Amino acids
- 1 to 403
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Nucleic acid-binding protein involved in innate immune response to DNA and RNA viruses (PubMed:30135424, PubMed:30193849). Binds DNA and RNA in the cytoplasm and acts by promoting recognition of viral nucleic acids by virus sensors, such as RIGI, IFIH1/MDA5 and CGAS (PubMed:30135424, PubMed:30193849). Acts as a co-sensor for recognition of double-stranded DNA (dsDNA) by cGAS in the cytoplasm, thereby playing a role in innate immune response to cytosolic dsDNA and DNA virus (PubMed:30135424). Binds dsDNA and probably acts by promoting sensing of dsDNA by CGAS, leading to enhance CGAS oligomerization and activation (PubMed:30135424). Promotes sensing of viral RNA by RIGI-like receptors proteins RIGI and IFIH1/MDA5 via two mechanisms: binds double-stranded RNA (dsRNA), enhancing the binding of RIGI and IFIH1/MDA5 to dsRNA and promotes 'Lys-63'-linked ubiquitination and subsequent activation of RIGI and IFIH1/MDA5 (PubMed:30193849).
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
U+00A0;
U+00A0;
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ZCCHC3 also known as Zinc Finger CCHC-type Containing 3 is a protein involved in RNA metabolism. It has a molecular mass of approximately 61 kDa. This protein is found in various tissues with high expression in the brain and immune-related organs such as the spleen. Mechanically ZCCHC3 binds to RNA molecules and influences their stability and processing. It has both CCHC-type zinc finger and RNA recognition motifs which contribute to its RNA binding capability.
- Biological function summary
ZCCHC3 plays a role in the immune response. It is linked to the sensing and signaling pathways that are triggered by viral infection. ZCCHC3 does not function independently as it associates with other molecules to form larger protein complexes. It assists in the recognition of viral RNA and helps activate downstream signaling pathways leading to the production of type I interferons and other cytokines that are critical elements of the antiviral response.
- Pathways
ZCCHC3 influences important immune signaling mechanisms. It is part of the RIG-I-like receptor (RLR) signaling pathway which detects viral RNAs and activates immune responses. In these pathways ZCCHC3 interacts with proteins such as RIG-I and MDA5. These interactions support antiviral defense mechanisms leading to the induction of interferon production and establishment of an antiviral state in infected and neighboring cells.
- Associated diseases and disorders
ZCCHC3 has connections to antiviral immune disorders. Alterations in its function might result in improper immune responses affecting viral clearance. Dysregulation in ZCCHC3 or its pathway partners like RIG-I can contribute to conditions such as systemic lupus erythematosus and other autoimmune diseases. Research indicates a potential link between ZCCHC3-related pathways and immune system dysregulation which could lead to heightened susceptibility to these disorders.
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1 product image
SDS-PAGE - Recombinant Human ZCCHC3 protein (ab164746)
ab164746 on a 12.5% SDS-PAGE stained with Coomassie Blue.
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