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Recombinant Human ZnT-8 protein is a Human Full Length protein, in the 1 to 369 aa range, expressed in Cell free, with >=85% purity and suitable for SDS-PAGE.

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Images

SDS-PAGE - Recombinant Human ZnT-8 protein (AB289751), expandable thumbnail

Key facts

Purity

>=85% SDS-PAGE

Expression system

Cell free

Tags

His tag N-Terminus

Applications

SDS-PAGE

Biologically active

No

Amino acid sequence

M E F L E R T Y L V N D K A A K M Y A F T L E S V E L Q Q K P V N K D Q C P R E R P E E L E S G G M Y H C H S G S K P T E K G A N E Y A Y A K W K L C S A S A I C F I F M I A E V V G G H I A G S L A V V T D A A H L L I D L T S F L L S L F S L W L S S K P P S K R L T F G W H R A E I L G A L L S I L C I W V V T G V L V Y L A C E R L L Y P D Y Q I Q A T V M I I V S S C A V A A N I V L T V V L H Q R C L G H N H K E V Q A N A S V R A A F V H A L G D L F Q S I S V L I S A L I I Y F K P E Y K I A D P I C T F I F S I L V L A S T I T I L K D F S I L L M E G V P K S L N Y S G V K E L I L A V D G V L S V H S L H I W S L T M N Q V I L S A H V A T A A S R D S Q V V R R E I A K A L S K S F T M H S L T I Q M E S P V D Q D P D C L F C E D P C D

Reactivity data

Application

SDS-PAGE

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Proton-coupled zinc ion antiporter mediating the entry of zinc into the lumen of pancreatic beta cell secretory granules, thereby regulating insulin secretion.

Alternative names

Recommended products

Recombinant Human ZnT-8 protein is a Human Full Length protein, in the 1 to 369 aa range, expressed in Cell free, with >=85% purity and suitable for SDS-PAGE.

Key facts

Purity

>=85% SDS-PAGE

Expression system

Cell free

Applications

SDS-PAGE

Accession
Q8IWU4-1
Animal free

Yes

Species

Human

Concentration
Loading...
Storage buffer

pH: 7.4 - 8
Constituents: 6% Trehalose, 0.87% Sodium chloride, 0.24% Tris, 0.05% 2-Octadecoxyethanol

Sequence info

Amino acid sequence

M E F L E R T Y L V N D K A A K M Y A F T L E S V E L Q Q K P V N K D Q C P R E R P E E L E S G G M Y H C H S G S K P T E K G A N E Y A Y A K W K L C S A S A I C F I F M I A E V V G G H I A G S L A V V T D A A H L L I D L T S F L L S L F S L W L S S K P P S K R L T F G W H R A E I L G A L L S I L C I W V V T G V L V Y L A C E R L L Y P D Y Q I Q A T V M I I V S S C A V A A N I V L T V V L H Q R C L G H N H K E V Q A N A S V R A A F V H A L G D L F Q S I S V L I S A L I I Y F K P E Y K I A D P I C T F I F S I L V L A S T I T I L K D F S I L L M E G V P K S L N Y S G V K E L I L A V D G V L S V H S L H I W S L T M N Q V I L S A H V A T A A S R D S Q V V R R E I A K A L S K S F T M H S L T I Q M E S P V D Q D P D C L F C E D P C D

Accession

Q8IWU4

Protein length

Full Length

Predicted molecular weight

43.6 kDa

Amino acids

1 to 369

Nature

Recombinant

Tags

His tag N-Terminus

Specifications

Form

Lyophilized

General info

Function

Proton-coupled zinc ion antiporter mediating the entry of zinc into the lumen of pancreatic beta cell secretory granules, thereby regulating insulin secretion.

Sequence similarities

Belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family. SLC30A subfamily.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Zinc transporter 8 (ZnT-8) also known as SLC30A8 is a protein that functions as a zinc transporter located in the membrane of insulin secretory vesicles in pancreatic beta cells. The molecular mass of ZnT-8 is approximately 51 kDa. Expression of ZnT-8 has been observed predominantly in the pancreas with significant presence in islet cells. ZnT-8 facilitates the movement of zinc ions from the cytoplasm into insulin granules which is essential for proper insulin crystallization and storage.

Biological function summary

Zinc plays an important role in insulin maturation and secretion and ZnT-8 contributes to these processes by regulating zinc ion homeostasis within the pancreatic beta cells. ZnT-8 is not part of a larger complex but operates alongside other zinc transporters to maintain zinc balance. This transporter ensures that zinc levels within insulin granules achieve conditions necessary for effective packaging and release of insulin.

Pathways

ZnT-8 is directly involved in regulating glucose homeostasis and insulin signaling pathways. The protein plays a significant part in the insulin secretion pathway enabling the correct response to fluctuating blood glucose levels. ZnT-8 activity is closely related to glucose transporter type 2 (GLUT2) and other components of the insulin signaling pathway which ensures the maintenance of normal blood glucose levels through appropriate insulin action and secretion.

Associated diseases and disorders

ZnT-8 has been closely associated with type 2 diabetes and autoimmune diabetes. Variants in the SLC30A8 gene which encodes ZnT-8 have been linked to altered risk of developing type 2 diabetes. This connection implicates ZnT-8 in the pathophysiology of impaired insulin function. Furthermore ZnT-8 is a significant autoantigen in autoimmune diabetes with antibodies against ZnT-8 (anti-ZnT8 antibodies) being detected in patients suggesting an immune response involving this protein in the disease process.

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1 product image

  • SDS-PAGE - Recombinant Human ZnT-8 protein (ab289751), expandable thumbnail

    SDS-PAGE - Recombinant Human ZnT-8 protein (ab289751)

    SDS-PAGE analysis of ab289751

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Product protocols

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