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AB162092

Recombinant Human Zonula occludens protein 3/ZO3 (GST tag N-Terminus)

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Recombinant Human Zonula occludens protein 3/ZO3 (GST tag N-Terminus) is a Human Fragment protein, in the 868 to 952 aa range, expressed in Wheat germ, suitable for ELISA, WB.

View Alternative Names

ZO3, TJP3, Tight junction protein ZO-3, Tight junction protein 3, Zona occludens protein 3, Zonula occludens protein 3

2 Images
SDS-PAGE - Recombinant Human Zonula occludens protein 3/ZO3 (GST tag N-Terminus) (AB162092)
  • SDS-PAGE

Lab

SDS-PAGE - Recombinant Human Zonula occludens protein 3/ZO3 (GST tag N-Terminus) (AB162092)

ab162092 on a 12.5% SDS-PAGE stained with Coomassie Blue.

SDS-PAGE - Recombinant Human Zonula occludens protein 3/ZO3 (GST tag N-Terminus) (AB162092)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human Zonula occludens protein 3/ZO3 (GST tag N-Terminus) (AB162092)

ab162092 on a 12.5% SDS-PAGE stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

WB, ELISA

applications

Biologically active

No

Accession

O95049

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Complementary Products

Primary Antibodies

AB181991

Anti-Zonula occludens protein 3/ZO3 antibody [EPR13414] - Plasma Membrane Marker

RabMAb|Recombinant|Lab Essentials|Trial Size

Immunocytochemistry/ Immunofluorescence - Anti-Zonula occludens protein 3/ZO3 antibody [EPR13414] - Plasma Membrane Marker (AB181991)

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  • 1
Proteins & Peptides

AB160078

Recombinant Human DBPA protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human DBPA protein (GST tag N-Terminus) (AB160078)

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  • 0
Proteins & Peptides

AB159886

Recombinant Human ZP2 protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human ZP2 protein (GST tag N-Terminus) (AB159886)

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  • 0
Proteins & Peptides

AB162411

Recombinant Human MSY2/YBOX2/YBX2 protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human MSY2/YBOX2/YBX2 protein (GST tag N-Terminus) (AB162411)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

This product was previously labelled as Zonula occludens protein 3.
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Sequence info

[{"sequence":"DEPPAPALARSSEPVQADESQSPRDRGRISAHQGAQVDSRHPQGQWRQDSMRTYEREALKKKFMRVHDAESSDEDGYDWGPATDL","proteinLength":"Fragment","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":952,"aminoAcidStart":868,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"O95049","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Zonula occludens protein 3 (ZO-3) also known as Tight junction protein 3 is a component of tight junction complexes located in epithelial and endothelial cells. ZO-3 exhibits a molecular mass of approximately 130 kDa. Within the cellular environment ZO-3 serves as a scaffold protein anchoring transmembrane proteins to the actin cytoskeleton maintaining tight junction integrity. Expressions of ZO-3 occur in tissues where tight junctions are present including the intestinal epithelium and blood-brain barrier.
Biological function summary

ZO-3 participates with other proteins in maintaining cellular polarity and regulating paracellular permeability. It forms a part of the tight junction complex where it interacts with proteins like ZO-1 and occludin. Through these interactions ZO-3 contributes to the junctional barrier between adjacent cells playing an essential role in controlling the passage of molecules and ions through these cellular layers. ZO-3 also helps in signal transduction pathways linked to cellular proliferation and differentiation.

Pathways

ZO-3 plays a significant role in tight junction signaling and the establishment of epithelial cell adhesion. Within these pathways ZO-3 collaborates with ZO-1 and other junctional proteins forming a network that regulates cellular responses to extracellular stimuli. These associations reinforce the epithelial barrier and help manage processes such as inflammation and tissue repair important for maintaining tissue homeostasis.

Zonula occludens protein 3 has a connection to conditions like inflammatory bowel disease (IBD) and certain cancers. Variations or disruptions in ZO-3 expression can weaken the intestinal barrier having potentially contributing effects on the development of IBD. Furthermore ZO-3 has an association with tumor suppressor proteins like ZO-1 suggesting that its dysfunction might play a role in the progression of cancerous growths by altering barrier properties and influencing cell proliferation.
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Specifications

Form

Liquid

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General info

Function

TJP1, TJP2, and TJP3 are closely related scaffolding proteins that link tight junction (TJ) transmembrane proteins such as claudins, junctional adhesion molecules, and occludin to the actin cytoskeleton (PubMed : 16129888). The tight junction acts to limit movement of substances through the paracellular space and as a boundary between the compositionally distinct apical and basolateral plasma membrane domains of epithelial and endothelial cells. Binds and recruits PATJ to tight junctions where it connects and stabilizes apical and lateral components of tight junctions (PubMed : 16129888). Promotes cell-cycle progression through the sequestration of cyclin D1 (CCND1) at tight junctions during mitosis which prevents CCND1 degradation during M-phase and enables S-phase transition (PubMed : 21411630). With TJP1 and TJP2, participates in the junctional retention and stability of the transcription factor DBPA, but is not involved in its shuttling to the nucleus (By similarity). Contrary to TJP2, TJP3 is dispensable for individual viability, embryonic development, epithelial differentiation, and the establishment of TJs, at least in the laboratory environment (By similarity).

Sequence similarities

Belongs to the MAGUK family.

Post-translational modifications

Phosphorylated (By similarity).

Subcellular localisation

Nucleus

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Product protocols

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Target data

TJP1, TJP2, and TJP3 are closely related scaffolding proteins that link tight junction (TJ) transmembrane proteins such as claudins, junctional adhesion molecules, and occludin to the actin cytoskeleton (PubMed : 16129888). The tight junction acts to limit movement of substances through the paracellular space and as a boundary between the compositionally distinct apical and basolateral plasma membrane domains of epithelial and endothelial cells. Binds and recruits PATJ to tight junctions where it connects and stabilizes apical and lateral components of tight junctions (PubMed : 16129888). Promotes cell-cycle progression through the sequestration of cyclin D1 (CCND1) at tight junctions during mitosis which prevents CCND1 degradation during M-phase and enables S-phase transition (PubMed : 21411630). With TJP1 and TJP2, participates in the junctional retention and stability of the transcription factor DBPA, but is not involved in its shuttling to the nucleus (By similarity). Contrary to TJP2, TJP3 is dispensable for individual viability, embryonic development, epithelial differentiation, and the establishment of TJs, at least in the laboratory environment (By similarity).
See full target information TJP3
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