Recombinant Human ZP2 protein is a Human Full Length protein, in the 1 to 736 aa range, expressed in Wheat germ and suitable for ELISA, WB.
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Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
Amino acid sequence
M A C R Q R G G S W S P S G W F N A G W S T Y R S I S L F F A L V T S G N S I D V S Q L V N P A F P G T V T C D E R E I T V E F P S S P G T K K W H A S V V D P L G L D M P N C T Y I L D P E K L T L R A T Y D N C T R R V H G G H Q M T I R V M N N S A A L R H G A V M Y Q F F C P A M Q V E E T Q G L S A S T I C Q K D F M S F S L P R V F S G L A D D S K G T K V Q M G W S I E V G D G A R A K T L T L P E A M K E G F S L L I D N H R M T F H V P F N A T G V T H Y V Q G N S H L Y M V S L K L T F I S P G Q K V I F S S Q A I C A P D P V T C N A T H M T L T I P E F P G K L K S V S F E N Q N I D V S Q L H D N G I D L E A T N G M K L H F S K T L L K T K L S E K C L L H Q F Y L A S L K L T F L L R P E T V S M V I Y P E C L C E S P V S I V T G E L C T Q D G F M D V E V Y S Y Q T Q P A L D L G T L R V G N S S C Q P V F E A Q S Q G L V R F H I P L N G C G T R Y K F E D D K V V Y E N E I H A L W T D F P P S K I S R D S E F R N D M L L N I N V E S L T P P V A S V K L G P F T L I L Q S Y P D N S Y Q Q P Y G E N E Y P L V R F L R Q P I Y M E V R V L N R D D P N I K L V L D D C W A T S T M D P D S F P Q W N V V V D G C A Y D L D N Y Q T T F H P V G S S V T H P D H Y Q R F D M K A F A F V S E A H V L S S L V Y F H C S A L I C N R L S P D S P L C S V T C P V S S R H R R A T G A T E A E K M T V S L P G P I L L L S D D S S F R G V G S S D L K A S G S S G E K S R S E T G E E V G S R G A M D T K G H K T A G D V G S K A V A A V A A F A G V V A T L G F I Y Y L Y E K R T V S N H
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Component of the zona pellucida, an extracellular matrix surrounding oocytes which mediates sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy (PubMed:29895852). The zona pellucida is composed of 3 to 4 glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP2 may act as a secondary sperm receptor (PubMed:29895852).
Alternative names
ZPA, ZP2, Zona pellucida sperm-binding protein 2, Zona pellucida glycoprotein 2, Zona pellucida protein A, Zp-2
Recommended products
Recombinant Human ZP2 protein is a Human Full Length protein, in the 1 to 736 aa range, expressed in Wheat germ and suitable for ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
- Accession
- Q05996-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- M A C R Q R G G S W S P S G W F N A G W S T Y R S I S L F F A L V T S G N S I D V S Q L V N P A F P G T V T C D E R E I T V E F P S S P G T K K W H A S V V D P L G L D M P N C T Y I L D P E K L T L R A T Y D N C T R R V H G G H Q M T I R V M N N S A A L R H G A V M Y Q F F C P A M Q V E E T Q G L S A S T I C Q K D F M S F S L P R V F S G L A D D S K G T K V Q M G W S I E V G D G A R A K T L T L P E A M K E G F S L L I D N H R M T F H V P F N A T G V T H Y V Q G N S H L Y M V S L K L T F I S P G Q K V I F S S Q A I C A P D P V T C N A T H M T L T I P E F P G K L K S V S F E N Q N I D V S Q L H D N G I D L E A T N G M K L H F S K T L L K T K L S E K C L L H Q F Y L A S L K L T F L L R P E T V S M V I Y P E C L C E S P V S I V T G E L C T Q D G F M D V E V Y S Y Q T Q P A L D L G T L R V G N S S C Q P V F E A Q S Q G L V R F H I P L N G C G T R Y K F E D D K V V Y E N E I H A L W T D F P P S K I S R D S E F R N D M L L N I N V E S L T P P V A S V K L G P F T L I L Q S Y P D N S Y Q Q P Y G E N E Y P L V R F L R Q P I Y M E V R V L N R D D P N I K L V L D D C W A T S T M D P D S F P Q W N V V V D G C A Y D L D N Y Q T T F H P V G S S V T H P D H Y Q R F D M K A F A F V S E A H V L S S L V Y F H C S A L I C N R L S P D S P L C S V T C P V S S R H R R A T G A T E A E K M T V S L P G P I L L L S D D S S F R G V G S S D L K A S G S S G E K S R S E T G E E V G S R G A M D T K G H K T A G D V G S K A V A A V A A F A G V V A T L G F I Y Y L Y E K R T V S N H
- Accession
- Q05996
- Protein length
- Full Length
- Amino acids
- 1 to 736
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Component of the zona pellucida, an extracellular matrix surrounding oocytes which mediates sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy (PubMed:29895852). The zona pellucida is composed of 3 to 4 glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP2 may act as a secondary sperm receptor (PubMed:29895852).
- Sequence similarities
Belongs to the ZP domain family. ZPA subfamily.
- Post-translational modifications
Proteolytically cleaved before the transmembrane segment to yield the secreted ectodomain incorporated in the zona pellucida.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
U+00A0;
U+00A0;
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ZP2 also known as zona pellucida glycoprotein 2 is a structural protein critical to the protective coat surrounding oocytes called the zona pellucida. The protein is approximately 68 kDa in mass. ZP2 is highly expressed in the ovary. This glycoprotein specifically contributes to the matrix of the zona pellucida with other related proteins forming a structure that enables various reproductive events like sperm binding and prevention of polyspermy.
- Biological function summary
ZP2 interacts with other zona pellucida proteins particularly ZP1 and ZP3 to form the zona pellucida matrix. This matrix allows the binding of sperm to the oocyte facilitating fertilization. The stability and integrity of this structure determine reproductive success and embryo development. ZP2 undergoes proteolytic cleavage during the fertilization process which is an important event for proper egg-sperm interaction.
- Pathways
ZP2 forms an integral part of the fertilization pathway where it directly interacts with proteins ZP1 and ZP3. These proteins create a framework critical for sperm recognition and binding. Once ZP2 undergoes cleavage it assists in the progression of the acrosome reaction an important biological pathway for sperm penetration of the zona pellucida. The interactions and modifications of ZP2 within these pathways highlight the importance of precise regulation in reproduction.
- Associated diseases and disorders
Mutations or abnormalities in ZP2 are linked to reproductive conditions such as infertility. These changes can lead to disrupted zona pellucida integrity which affects sperm binding and fertilization ability. Additionally abnormalities in ZP2 relate to oocyte maturation defects impacting reproductive health. The protein also shows interplay with ZP3 in these conditions underlining its collaborative role in maintaining fertility.
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1 product image
SDS-PAGE - Recombinant Human ZP2 protein (ab159886)
ab159886 on a 12.5% SDS-PAGE stained with Coomassie Blue.
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