Recombinant Human ZP3 protein (His tag) is a Human Fragment protein, in the 23 to 387 aa range, expressed in Mammalian, with >90% purity and suitable for SDS-PAGE.
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Key facts
- Purity
>90% SDS-PAGE
- Expression system
Mammalian
- Tags
His tag N-Terminus
- Applications
SDS-PAGE
- Biologically active
No
Amino acid sequence
Q P L W L L Q G G A S H P E T S V Q P V L V E C Q E A T L M V M V S K D L F G T G K L I R A A D L T L G P E A C E P L V S M D T E D V V R F E V G L H E C G N S M Q V T D D A L V Y S T F L L H D P R P V G N L S I V R T N R A E I P I E C R Y P R Q G N V S S Q A I L P T W L P F R T T V F S E E K L T F S L R L M E E N W N A E K R S P T F H L G D A A H L Q A E I H T G S H V P L R L F V D H C V A T P T P D Q N A S P Y H T I V D F H G C L V D G L T D A S S A F K V P R P G P D T L Q F T V D V F H F A N D S R N M I Y I T C H L K V T L A E Q D P D E L N K A C S F S K P S N S W F P V E G S A D I C Q C C N K G D C G T P S H S R R Q P H V M S Q W S R S A S R N R R H V T E E A D V T V G P L I F L D R R G D H E V E Q W A L P S D T S V
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Component of the zona pellucida, an extracellular matrix surrounding oocytes which mediates sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy. The zona pellucida is composed of 3 to 4 glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP3 is essential for sperm binding and zona matrix formation.
Alternative names
Zona pellucida sperm-binding protein 3, Sperm receptor, ZP3A/ZP3B, Zona pellucida glycoprotein 3, Zona pellucida protein C, Zp-3, ZP3A, ZP3B, ZP3, ZPC
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Recombinant Human ZP3 protein (His tag) is a Human Fragment protein, in the 23 to 387 aa range, expressed in Mammalian, with >90% purity and suitable for SDS-PAGE.
Key facts
- Purity
>90% SDS-PAGE
- Expression system
Mammalian
- Tags
His tag N-Terminus
- Applications
SDS-PAGE
- Biologically active
No
- Accession
- P21754-1
- Animal free
No
- Species
Human
- Concentration
- Storage buffer
pH: 7.2 - 7.4
Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
Q P L W L L Q G G A S H P E T S V Q P V L V E C Q E A T L M V M V S K D L F G T G K L I R A A D L T L G P E A C E P L V S M D T E D V V R F E V G L H E C G N S M Q V T D D A L V Y S T F L L H D P R P V G N L S I V R T N R A E I P I E C R Y P R Q G N V S S Q A I L P T W L P F R T T V F S E E K L T F S L R L M E E N W N A E K R S P T F H L G D A A H L Q A E I H T G S H V P L R L F V D H C V A T P T P D Q N A S P Y H T I V D F H G C L V D G L T D A S S A F K V P R P G P D T L Q F T V D V F H F A N D S R N M I Y I T C H L K V T L A E Q D P D E L N K A C S F S K P S N S W F P V E G S A D I C Q C C N K G D C G T P S H S R R Q P H V M S Q W S R S A S R N R R H V T E E A D V T V G P L I F L D R R G D H E V E Q W A L P S D T S V
- Accession
- Protein length
Fragment
- Predicted molecular weight
44.6 kDa
- Amino acids
23 to 387
- Nature
Recombinant
- Tags
His tag N-Terminus
Specifications
- Form
Liquid
General info
- Function
Component of the zona pellucida, an extracellular matrix surrounding oocytes which mediates sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy. The zona pellucida is composed of 3 to 4 glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP3 is essential for sperm binding and zona matrix formation.
- Sequence similarities
Belongs to the ZP domain family. ZPC subfamily.
- Post-translational modifications
Proteolytically cleaved before the transmembrane segment to yield the secreted ectodomain incorporated in the zona pellucida.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ZP3 also known as Zona Pellucida Glycoprotein 3 or ZPA is a glycoprotein with a molecular mass of approximately 55-65 kDa. It is located in the extracellular matrix of the zona pellucida a glycoprotein layer that surrounds the plasma membrane of mammalian oocytes. ZP3 plays a significant role in the early stages of fertilization. It acts as a sperm receptor and is found in ovarian tissues where it is expressed during oocyte maturation.
- Biological function summary
ZP3 functions as an important part of the zona pellucida the structure required for sperm binding and induction of the acrosome reaction. It operates as a part of a glycoprotein complex including ZP1 and ZP2 which helps maintain the integrity and function of the zona pellucida. The binding of sperm to ZP3 initiates signaling cascades that enable sperm to penetrate the egg key for successful fertilization. Therefore ZP3's role is essential for reproduction.
- Pathways
The engagement of ZP3 in the fertilization process ties it to reproductive signaling pathways such as those involving gamete recognition and binding. It is related to proteins like sperm surface receptors which interact with ZP3 to trigger pathways that lead to the acrosome reaction. The interaction between ZP3 and these receptors marks an initial step in the signaling cascade necessary for sperm entry into the oocyte.
- Associated diseases and disorders
Alterations in ZP3 expression or function can impact fertility leading to conditions such as infertility or abnormal zona pellucida formation. Mutations in ZP3 and associated proteins like ZP1 and ZP2 disrupt the structural integrity of the zona pellucida thereby affecting sperm binding or penetration. Such disruptions can result in failure of fertilization highlighting the key role of ZP3 and its interactions in maintaining reproductive health.
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1 product image
SDS-PAGE - Recombinant Human ZP3 protein (His tag) (ab226286)
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) analysis of ab226286 with 5% enrichment gel and 15% separation gel.
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