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AB181888

Recombinant Human ZWILCH protein (His tag N-Terminus)

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Recombinant Human ZWILCH protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 591 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE.

View Alternative Names

Protein zwilch homolog, hZwilch, ZWILCH

2 Images
SDS-PAGE - Recombinant Human ZWILCH protein (His tag N-Terminus) (AB181888)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human ZWILCH protein (His tag N-Terminus) (AB181888)
SDS-PAGE - Recombinant Human ZWILCH protein (His tag N-Terminus) (AB181888)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human ZWILCH protein (His tag N-Terminus) (AB181888)

15% SDS-PAGE analysis of 3ug ab181888.

Key facts

Purity

>85% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q9H900

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMWERLNCAAEDFYSRLLQKFNEEKKGIRKDPFLYEADVQVQLISKGQPNPLKNILNENDIVFIVEKVPLEKEETSHIEELQSEETAISDFSTGENVGPLALPVGKARQLIGLYTMAHNPNMTHLKINLPVTALPPLWVRCDSSDPEGTCWLGAELITTNNSITGIVLYVVSCKADKNYSVNLENLKNLHKKRHHLSTVTSKGFAQYELFKSSALDDTITASQTAIALDISWSPVDEILQIPPLSSTATLNIKVESGEPRGPLNHLYRELKFLLVLADGLRTGVTEWLEPLEAKSAVELVQEFLNDLNKLDGFGDSTKKDTEVETLKHDTAAVDRSVKRLFKVRSDLDFAEQLWCKMSSSVISYQDLVKCFTLIIQSLQRGDIQPWLHSGSNSLLSKLIHQSYHGTMDTVSLSGTIPVQMLLEIGLDKLKKDYISFFIGQELASLNHLEYFIAPSVDIQEQVYRVQKLHHILEILVSCMPFIKSQHELLFSLTQICIKYYKQNPLDEQHIFQLPVRPTAVKNLYQSEKPQKWRVEIYSGQKKIKTVWQLSDSSPIDHLNFHKPDFSELTLNGSLEERIFFTNMVTCSQVHFK","proteinLength":"Full Length","predictedMolecularWeight":"69.6 kDa","actualMolecularWeight":null,"aminoAcidEnd":591,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9H900","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ZWILCH also known as ZW10 interactor-like or ZWILCH kinetochore protein is an important component in cell division especially during mitosis. The protein has a molecular mass of approximately 85 kDa. ZWILCH is highly expressed in dividing cells with enrichment in tissues that have high mitotic rates like the bone marrow and germinal centers. Functionally ZWILCH is a component of the RZZ complex comprising ROD (also known as KNTC1) and ZW10 helping in the proper attachment of chromosomes at the kinetochores.
Biological function summary

ZWILCH is fundamental in ensuring accurate chromosome alignment and segregation during cell division. It plays a part as a component of the RZZ complex. The RZZ complex localizes to kinetochores which are critical for connecting spindle microtubules to chromosomes promoting bioriented chromosome alignment. Moreover ZWILCH contributes to the spindle assembly checkpoint a safety mechanism that prevents chromosome mis-segregation by delaying progress to anaphase until all chromosomes are correctly attached.

Pathways

ZWILCH plays integral roles in the mitotic spindle checkpoint and chromosomal stability pathways. Within these pathways ZWILCH associates closely with spindle assembly checkpoint proteins such as MAD2 and BUBR1 which monitor kinetochore-microtubule attachment. ZWILCH through its role in the RZZ complex communicates tension status between sister chromatids ensuring proper cell-cycle progression and genomic integrity.

ZWILCH has significant connections to both cancer and aneuploidy. Misregulation or mutations in ZWILCH can result in chromosomal instability a main feature in various cancers. Aberrant activity of the RZZ complex where ZWILCH is vital could disrupt normal cell division and cause aneuploidy a condition characterized by an abnormal number of chromosomes seen in numerous cancer types and genetic disorders. Therefore ZWILCH and its associations with proteins like ZW10 and ROD serve as potential biomarkers for chromosomal instability-related diseases.
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Specifications

Form

Liquid

Additional notes

ab181888 was purified using conventional chromatography techniques.

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General info

Function

Essential component of the mitotic checkpoint, which prevents cells from prematurely exiting mitosis. Required for the assembly of the dynein-dynactin and MAD1-MAD2 complexes onto kinetochores. Its function related to the spindle assembly machinery is proposed to depend on its association in the mitotic RZZ complex (PubMed : 15824131).

Sequence similarities

Belongs to the ZWILCH family.

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Product protocols

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Target data

Essential component of the mitotic checkpoint, which prevents cells from prematurely exiting mitosis. Required for the assembly of the dynein-dynactin and MAD1-MAD2 complexes onto kinetochores. Its function related to the spindle assembly machinery is proposed to depend on its association in the mitotic RZZ complex (PubMed : 15824131).
See full target information ZWILCH
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Complementary Products

Primary Antibodies

AB202898

Anti-ZWILCH antibody [EPR18035-28]

RabMAb|Recombinant

Immunoprecipitation - Anti-ZWILCH antibody [EPR18035-28] (AB202898)

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