Recombinant Human ZWINT protein is a Human Full Length protein, in the 1 to 230 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE, MS.
Images
Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S M E A A E T E A E A A A L E V L A E V A G I L E P V G L Q E E A E L P A K I L V E F V V D S Q K K D K L L C S Q L Q V A D F L Q N I L A Q E D T A K G L D P L A S E D T S R Q K A I A A K E Q W K E L K A T Y R E H V E A I K I G L T K A L T Q M E E A Q R K R T Q L R E A F E Q L Q A K K Q M A M E K R R A V Q N Q W Q L Q Q E K H L Q H L A E V S A E G K L L F P E A E A E A E N L P D D K P Q Q P T R P Q E Q S T G D T M G R D P G V S F K A V G L Q P A G D V N L P
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Acts as a component of the outer kinetochore KNL1 complex that serves as a docking point for spindle assembly checkpoint components and mediates microtubule-kinetochore interactions (PubMed:15094189, PubMed:15485811, PubMed:15824131, PubMed:16732327, PubMed:24530301, PubMed:27881301, PubMed:38459127, PubMed:38459128). Kinetochores, consisting of a centromere-associated inner segment and a microtubule-contacting outer segment, play a crucial role in chromosome segregation by mediating the physical connection between centromeric DNA and spindle microtubules (PubMed:15094189, PubMed:15485811, PubMed:16732327). The outer kinetochore is made up of the ten-subunit KMN network, comprising the MIS12, NDC80 and KNL1 complexes, and auxiliary microtubule-associated components; together they connect the outer kinetochore with the inner kinetochore, bind microtubules, and mediate interactions with mitotic checkpoint proteins that delay anaphase until chromosomes are bioriented on the spindle (PubMed:15094189, PubMed:15485811, PubMed:15824131, PubMed:16732327, PubMed:24530301, PubMed:38459127, PubMed:38459128). Targets the RZZ complex to the kinetochore at prometaphase (PubMed:15485811). Recruits MAD2L1 to the kinetochore, but is not required for BUB1B localization (By similarity). In addition to orienting mitotic chromosomes, it is also essential for alignment of homologous chromosomes during meiotic metaphase I (By similarity). In meiosis I, required to activate the spindle assembly checkpoint at unattached kinetochores to correct erroneous kinetochore-microtubule attachments (PubMed:15485811).
Alternative names
Outer kinetochore KNL1 complex subunit ZWINT, ZW10 interactor, ZW10-interacting protein 1, Zwint-1, ZWINT
Recommended products
Recombinant Human ZWINT protein is a Human Full Length protein, in the 1 to 230 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- O95229-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 20% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S M E A A E T E A E A A A L E V L A E V A G I L E P V G L Q E E A E L P A K I L V E F V V D S Q K K D K L L C S Q L Q V A D F L Q N I L A Q E D T A K G L D P L A S E D T S R Q K A I A A K E Q W K E L K A T Y R E H V E A I K I G L T K A L T Q M E E A Q R K R T Q L R E A F E Q L Q A K K Q M A M E K R R A V Q N Q W Q L Q Q E K H L Q H L A E V S A E G K L L F P E A E A E A E N L P D D K P Q Q P T R P Q E Q S T G D T M G R D P G V S F K A V G L Q P A G D V N L P
- Accession
- O95229
- Protein length
- Full Length
- Predicted molecular weight
- 27.9 kDa
- Amino acids
- 1 to 230
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Acts as a component of the outer kinetochore KNL1 complex that serves as a docking point for spindle assembly checkpoint components and mediates microtubule-kinetochore interactions (PubMed:15094189, PubMed:15485811, PubMed:15824131, PubMed:16732327, PubMed:24530301, PubMed:27881301, PubMed:38459127, PubMed:38459128). Kinetochores, consisting of a centromere-associated inner segment and a microtubule-contacting outer segment, play a crucial role in chromosome segregation by mediating the physical connection between centromeric DNA and spindle microtubules (PubMed:15094189, PubMed:15485811, PubMed:16732327). The outer kinetochore is made up of the ten-subunit KMN network, comprising the MIS12, NDC80 and KNL1 complexes, and auxiliary microtubule-associated components; together they connect the outer kinetochore with the inner kinetochore, bind microtubules, and mediate interactions with mitotic checkpoint proteins that delay anaphase until chromosomes are bioriented on the spindle (PubMed:15094189, PubMed:15485811, PubMed:15824131, PubMed:16732327, PubMed:24530301, PubMed:38459127, PubMed:38459128). Targets the RZZ complex to the kinetochore at prometaphase (PubMed:15485811). Recruits MAD2L1 to the kinetochore, but is not required for BUB1B localization (By similarity). In addition to orienting mitotic chromosomes, it is also essential for alignment of homologous chromosomes during meiotic metaphase I (By similarity). In meiosis I, required to activate the spindle assembly checkpoint at unattached kinetochores to correct erroneous kinetochore-microtubule attachments (PubMed:15485811).
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ZWINT also known as ZW10 interactor is a protein that plays an important role in the attachment of kinetochores to the spindle microtubules during mitosis. It has a molecular weight of approximately 35 kDa. This protein is broadly expressed in various tissues with especially high presence in dividing cells reflecting its importance in cell division processes.
- Biological function summary
ZWINT functions as a part of the kinetochore complex which is essential for chromosome segregation during mitotic cell division. It interacts with other kinetochore proteins to facilitate the correct attachment of chromosomes to the spindle apparatus. This interaction ensures that chromosomes are accurately distributed to daughter cells maintaining genomic stability.
- Pathways
The ZWINT protein is integral in the mitotic spindle checkpoint pathway also known as the spindle assembly checkpoint. It works closely with proteins such as ZW10 and Rod to monitor and regulate the attachment of spindle microtubules to the kinetochores preventing aneuploidy during cell division. This pathway ensures that mitosis does not proceed until all chromosomes are properly aligned and attached contributing to the fidelity of cell division.
- Associated diseases and disorders
Mutations or dysregulation in ZWINT have been linked to certain cancers due to its influence on chromosome segregation. Disruptions in ZWINT function might also contribute to chromosomal instability which can lead to tumorigenesis. In cancerous contexts proteins such as Mad2 and BubR1 which are part of the spindle assembly checkpoint pathway also show altered interactions highlighting a complex network of regulatory proteins involved in maintaining cellular homeostasis.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
SDS-PAGE - Recombinant Human ZWINT protein (ab180284)
15% SDS-PAGE analysis of ab180284 (3μg).
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com