Recombinant Influenza A H1N1 Hemagglutinin Protein (His-tag) is a Influenza A virus (A/Puerto Rico/8/1934(H1N1)) Fragment protein, in the 18 to 528 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for HPLC, SDS-PAGE, MS.
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Key facts
- Purity
>=95% HPLC
- Endotoxin level
<= 0.005 EU/µg
- Expression system
HEK 293 cells
- Tags
His tag C-Terminus
- Applications
HPLC, SDS-PAGE, MS
- Biologically active
No
Amino acid sequence
D T I C I G Y H A N N S T D T V D T V L E K N V T V T H S V N L L E D S H N G K L C R L K G I A P L Q L G K C N I A G W L L G N P E C D P L L P V R S W S Y I V E T P N S E N G I C Y P G D F I D Y E E L R E Q L S S V S S F E R F E I F P K E S S W P N H N T N G V T A A C S H E G K S S F Y R N L L W L T E K E G S Y P K L K N S Y V N K K G K E V L V L W G I H H P P N S K E Q Q N L Y Q N E N A Y V S V V T S N Y N R R F T P E I A E R P K V R D Q A G R M N Y Y W T L L K P G D T I I F E A N G N L I A P M Y A F A L S R G F G S G I I T S N A S M H E C N T K C Q T P L G A I N S S L P Y Q N I H P V T I G E C P K Y V R S A K L R M V T G L R N I P S I Q S R G L F G A I A G F I E G G W T G M I D G W Y G Y H H Q N E Q G S G Y A A D Q K S T Q N A I N G I T N K V N T V I E K M N I Q F T A V G K E F N K L E K R M E N L N K K V D D G F L D I W T Y N A E L L V L L E N E R T L D F H D S N V K N L Y E K V K S Q L K N N A K E I G N G C F E F Y H K C D N E C M E S V R N G T Y D Y P K Y S E E S K L N R E K V D G V K L E S M G I Y Q
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization either through clathrin-dependent endocytosis or through clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore.
Alternative names
Hemagglutinin, HA
Recommended products
Recombinant Influenza A H1N1 Hemagglutinin Protein (His-tag) is a Influenza A virus (A/Puerto Rico/8/1934(H1N1)) Fragment protein, in the 18 to 528 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for HPLC, SDS-PAGE, MS.
Key facts
- Purity
>=95% HPLC
- Endotoxin level
<= 0.005 EU/µg
- Expression system
HEK 293 cells
- Tags
His tag C-Terminus
- Applications
HPLC, SDS-PAGE, MS
- Biologically active
No
- Accession
- P03452-1
- Animal free
Yes
- Species
Influenza A virus (A/Puerto Rico/8/1934(H1N1))
- Reconstitution
Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product. Store lyophilized form at room temperature. Reconstitute in phosphate buffered saline, aliquot and store at -80°C for 12 months or +4°C for 1 week. Avoid repeated freeze-thaw.
- Concentration
- Storage buffer
pH: 7.4
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic
Sequence info
Amino acid sequence
D T I C I G Y H A N N S T D T V D T V L E K N V T V T H S V N L L E D S H N G K L C R L K G I A P L Q L G K C N I A G W L L G N P E C D P L L P V R S W S Y I V E T P N S E N G I C Y P G D F I D Y E E L R E Q L S S V S S F E R F E I F P K E S S W P N H N T N G V T A A C S H E G K S S F Y R N L L W L T E K E G S Y P K L K N S Y V N K K G K E V L V L W G I H H P P N S K E Q Q N L Y Q N E N A Y V S V V T S N Y N R R F T P E I A E R P K V R D Q A G R M N Y Y W T L L K P G D T I I F E A N G N L I A P M Y A F A L S R G F G S G I I T S N A S M H E C N T K C Q T P L G A I N S S L P Y Q N I H P V T I G E C P K Y V R S A K L R M V T G L R N I P S I Q S R G L F G A I A G F I E G G W T G M I D G W Y G Y H H Q N E Q G S G Y A A D Q K S T Q N A I N G I T N K V N T V I E K M N I Q F T A V G K E F N K L E K R M E N L N K K V D D G F L D I W T Y N A E L L V L L E N E R T L D F H D S N V K N L Y E K V K S Q L K N N A K E I G N G C F E F Y H K C D N E C M E S V R N G T Y D Y P K Y S E E S K L N R E K V D G V K L E S M G I Y Q
- Accession
- Protein length
Fragment
- Predicted molecular weight
59.26 kDa
- Actual molecular weight
59.27 kDa
- Amino acids
18 to 528
- Nature
Recombinant
- Tags
His tag C-Terminus
Specifications
- Form
Lyophilized
- Additional notes
SDS-PAGE >= 95%
General info
- Function
Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization either through clathrin-dependent endocytosis or through clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore.
- Sequence similarities
Belongs to the influenza viruses hemagglutinin family.
- Post-translational modifications
Palmitoylated.
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
Ambient
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Influenza A Virus Hemagglutinin also known as hemagglutinin or influenza HA is a surface glycoprotein found on the influenza A virus. Hemagglutinin has a mass of approximately 75 kDa and serves as an important player in the virus's ability to infect host cells. It is expressed on the exterior membrane of the influenza virus and allows the virus to attach to and enter host cells initiating the infection process. This protein comes in various subtypes adding complexity to the virus's infection strategies and complicating vaccine development.
- Biological function summary
Hemagglutinin facilitates the binding of the virus to sialic acid receptors on the surface of host epithelial cells. This binding triggers endocytosis allowing the virus entry into the host cell. Hemagglutinin is part of a complex that works closely with neuraminidase another glycoprotein on the virus surface. The hemagglutinin-neuraminidase complex is essential for the viral life cycle balancing attachment to host cells with the release of new viral particles.
- Pathways
Hemagglutinin plays a significant role in the viral infection pathway of the influenza virus. It is critical in the initial steps of the pathway that involve viral entry and fusion with the host cell membrane. Hemagglutinin's interaction with the sialic acid receptors is closely linked with the activity of neuraminidase which aids in the release stage of the infection. This interplay between hemagglutinin and neuraminidase highlights their participation in the viral replication cycle demonstrating their complementary roles.
- Associated diseases and disorders
Hemagglutinin is deeply connected to seasonal influenza outbreaks and pandemics. Alterations in the hemagglutinin protein such as mutations and antigenic drifts or shifts can lead to new influenza strains with pandemic potential. The interaction of hemagglutinin with host immune responses and its capability to change allows the virus to evade immune detection. Neuraminidase inhibitors a class of antiviral drugs target enzymes closely related to hemagglutinin to prevent the spread of infection showcasing hemagglutinin's centrality in both infection and therapeutic intervention strategies.
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3 product images
Mass Spectrometry - Recombinant Influenza A H1N1 Hemagglutinin Protein (His-tag) (ab307788)
Mass determination by ESI-TOF. Predicted MW is 59263.11 Da (+/- 10 Da by ESI-TOF). Observed MW is 59269.29 Da.
SDS-PAGE - Recombinant Influenza A H1N1 Hemagglutinin Protein (His-tag) (ab307788)
SDS-PAGE analysis of ab307788
HPLC - Recombinant Influenza A H1N1 Hemagglutinin Protein (His-tag) (ab307788)
HPLC analysis of ab307788
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