Recombinant Influenza A Hemagglutinin protein (His tag) is a Influenza A virus (A/Hickox/1940(H1N1)) Full Length protein, in the 18 to 343 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
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Key facts
- Purity
>90% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE
- Biologically active
No
Amino acid sequence
D T I C I G Y H A N N S T D T V D T V L E K N V T V T H S V N L L E D S H N G K L C R L K G I A P L Q L G K C N I A G W I L G N P E C E S L L S K R S W S Y I A E T P N S E N G T C Y P G D F A D Y E E L R E Q L S S V S S F E R F E I F P K E R S W P N H N I N I G V T A A C S H A G K S S F Y K N L L W L T E K D G S Y P N L N K S Y V N K K E K E V L V L W G V H H P S N I E N Q K T L Y R K E N A Y V S V V S S N Y N R R F T P E I A E R P K V R G Q A G R M N Y Y W T L L E P G D T I I F E A N G N L I A P W Y A F A L S R G L G S G I I T S N A S M D E C D T K C Q T P Q G A I N S S L P F Q N I H P F T I G E C P K Y V R S T K L R M V T G L R N I P S I Q S
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization either through clathrin-dependent endocytosis or through clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore.
Alternative names
Hemagglutinin, HA
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Recombinant Influenza A Hemagglutinin protein (His tag) is a Influenza A virus (A/Hickox/1940(H1N1)) Full Length protein, in the 18 to 343 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
Alternative names
Hemagglutinin, HA
Key facts
- Purity
>90% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE
- Biologically active
No
- Accession
- Q0HD60-1
- Animal free
No
- Species
Influenza A virus (A/Hickox/1940(H1N1))
- Concentration
- Storage buffer
pH: 7.2 - 7.4
Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
D T I C I G Y H A N N S T D T V D T V L E K N V T V T H S V N L L E D S H N G K L C R L K G I A P L Q L G K C N I A G W I L G N P E C E S L L S K R S W S Y I A E T P N S E N G T C Y P G D F A D Y E E L R E Q L S S V S S F E R F E I F P K E R S W P N H N I N I G V T A A C S H A G K S S F Y K N L L W L T E K D G S Y P N L N K S Y V N K K E K E V L V L W G V H H P S N I E N Q K T L Y R K E N A Y V S V V S S N Y N R R F T P E I A E R P K V R G Q A G R M N Y Y W T L L E P G D T I I F E A N G N L I A P W Y A F A L S R G L G S G I I T S N A S M D E C D T K C Q T P Q G A I N S S L P F Q N I H P F T I G E C P K Y V R S T K L R M V T G L R N I P S I Q S
- Protein length
Full Length
- Predicted molecular weight
52.6 kDa
- Amino acids
18 to 343
- Nature
Recombinant
- Tags
His tag N-Terminus
Specifications
- Form
Liquid
General info
- Function
Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization either through clathrin-dependent endocytosis or through clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore.
- Sequence similarities
Belongs to the influenza viruses hemagglutinin family.
- Post-translational modifications
Palmitoylated.
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Supplementary info
- Activity summary
Influenza A Hemagglutinin often called hemagglutinin or the HA protein is a glycoprotein found on the surface of the Influenza A virus. It plays a mechanical role in the viral entry by binding to sialic acid residues on host cells. This binding allows the virus to attach and initiate infection. Hemagglutinin is known for its significant molecular mass of approximately 75 kDa. It expresses in various strains of the Influenza A virus including the Hong Kong strain and A/California/07/2009. It is an important component in determining the virus's ability to infect different host species.
- Biological function summary
The HA protein functions in a critical step of the viral life cycle called fusion. During the infection process hemagglutinin undergoes a structural change that facilitates the fusion of the viral envelope with the host cell membrane. This fusion process is essential to release the viral genome into the host cell. Hemagglutinin generally operates as part of a trimeric complex which enhances its ability to interact with host cell receptors. This interaction is vital for the virus to efficiently invade the host cells and propagate the infection throughout the host.
- Pathways
Hemagglutinin plays an important role in the viral fusion pathway and the innate immune response pathway. It interacts with proteins like neuraminidase another surface protein of the influenza virus which assists in the release of progeny viruses from infected cells. During the fusion pathway HA's structural changes allow the viral and cellular membranes to merge a step necessary for the virus to establish infection. Its involvement in the immune response pathway also prompts the host immune system to recognize and respond to viral threats.
- Associated diseases and disorders
Hemagglutinin is closely associated with influenza a contagious respiratory illness caused by influenza viruses. Mutations or antigenic shifts in HA contribute to seasonal flu epidemics and pandemics. The HA protein is a significant target for antibodies because its alteration can lead to vaccine inefficacy. Additionally it is associated with immune escape mechanisms where structural changes reduce antibody recognition. Researchers often study the interaction of hemagglutinin and antibodies like those targeting neuraminidase as these interactions are important for developing effective vaccines and antiviral therapies.
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SDS-PAGE - Recombinant Influenza A Hemagglutinin protein (His tag) (ab225645)
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) analysis of ab225645 with 5% enrichment gel and 15% separation gel.
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