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AB217662

Recombinant Influenza A Hemagglutinin protein (His tag) Strain California/07/2009/H1N1

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Recombinant Influenza A Hemagglutinin protein (His tag) Strain California/07/2009/H1N1 is a Influenza A virus (A/Hickox/1940(H1N1)) Fragment protein, in the 18 to 345 aa range, expressed in HEK 293 cells, with >95%, < 0.01 EU/µg endotoxin level, suitable for SDS-PAGE.

View Alternative Names

Hemagglutinin, HA

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SDS-PAGE - Recombinant Influenza A Hemagglutinin protein (His tag) Strain California/07/2009/H1N1 (AB217662)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Influenza A Hemagglutinin protein (His tag) Strain California/07/2009/H1N1 (AB217662)

12% SDS-PAGE analysis of ab217662.

Lane 1 : Reducing condition.

Lane 2 : Non-reducing condition.

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

< 0.01 EU/µg

Expression system

HEK 293 cells

Tags

His tag C-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q0HD60

Animal free

No

Carrier free

No

Species

Influenza A virus (A/Hickox/1940(H1N1))

Storage buffer

Constituents: PBS

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"DTLCIGYHANNSTDTVDTVLEKNVTVTHSVNLLEDKHNGKLCKLRGVAPLHLGKCNIAGWILGNPECESLSTASSWSYIVETPSSDNGTCYPGDFIDYEELREQLSSVSSFERFEIFPKTSSWPNHDSNKGVTAACPHAGAKSFYKNLIWLVKKGNSYPKLSKSYINDKGKEVLVLWGIHHPSTSADQQSLYQNADAYVFVGSSRYSKKFKPEIAIRPKVRDQEGRMNYYWTLVEPGDKITFEATGNLVVPRYAFAMERNAGSGIIISDTPVHDCNTTCQTPKGAINTSLPFQNIHPITIGKCPKYVKSTKLRLATGLRNIPSIQSRGHHHHHH","proteinLength":"Fragment","predictedMolecularWeight":"37.19 kDa","actualMolecularWeight":null,"aminoAcidEnd":345,"aminoAcidStart":18,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":null,"tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Influenza A Hemagglutinin often called hemagglutinin or the HA protein is a glycoprotein found on the surface of the Influenza A virus. It plays a mechanical role in the viral entry by binding to sialic acid residues on host cells. This binding allows the virus to attach and initiate infection. Hemagglutinin is known for its significant molecular mass of approximately 75 kDa. It expresses in various strains of the Influenza A virus including the Hong Kong strain and A/California/07/2009. It is an important component in determining the virus's ability to infect different host species.
Biological function summary

The HA protein functions in a critical step of the viral life cycle called fusion. During the infection process hemagglutinin undergoes a structural change that facilitates the fusion of the viral envelope with the host cell membrane. This fusion process is essential to release the viral genome into the host cell. Hemagglutinin generally operates as part of a trimeric complex which enhances its ability to interact with host cell receptors. This interaction is vital for the virus to efficiently invade the host cells and propagate the infection throughout the host.

Pathways

Hemagglutinin plays an important role in the viral fusion pathway and the innate immune response pathway. It interacts with proteins like neuraminidase another surface protein of the influenza virus which assists in the release of progeny viruses from infected cells. During the fusion pathway HA's structural changes allow the viral and cellular membranes to merge a step necessary for the virus to establish infection. Its involvement in the immune response pathway also prompts the host immune system to recognize and respond to viral threats.

Hemagglutinin is closely associated with influenza a contagious respiratory illness caused by influenza viruses. Mutations or antigenic shifts in HA contribute to seasonal flu epidemics and pandemics. The HA protein is a significant target for antibodies because its alteration can lead to vaccine inefficacy. Additionally it is associated with immune escape mechanisms where structural changes reduce antibody recognition. Researchers often study the interaction of hemagglutinin and antibodies like those targeting neuraminidase as these interactions are important for developing effective vaccines and antiviral therapies.
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Specifications

Form

Liquid

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General info

Function

Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization either through clathrin-dependent endocytosis or through clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore.

Sequence similarities

Belongs to the influenza viruses hemagglutinin family.

Post-translational modifications

Palmitoylated.. In natural infection, inactive HA is matured into HA1 and HA2 outside the cell by one or more trypsin-like, arginine-specific endoprotease secreted by the bronchial epithelial cells. One identified protease that may be involved in this process is secreted in lungs by club cells.

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Product protocols

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Target data

Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization either through clathrin-dependent endocytosis or through clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore.
See full target information HA

Additional targets

Influenza A Hemagglutinin
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