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AB224859

Recombinant Influenza A Influenza A Virus Nucleoprotein (His tag)

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(1 Publication)

Recombinant Influenza A Influenza A Virus Nucleoprotein (His tag) is a Influenza A virus (A/Beijing/353/1989(H3N2)) Full Length protein, in the 1 to 498 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

Nucleoprotein, Nucleocapsid protein, Protein N, NP, Common flu NP, Influenza A virus NP, NP, Nucleocapsid protein, Nucleoprotein, Protein N, Seasonal Influenza A (H1N1) Nucleocapsid Protein

3 Images
Mass Spectrometry - Recombinant Influenza A Influenza A Virus Nucleoprotein (His tag) (AB224859)
  • Mass Spec

Supplier Data

Mass Spectrometry - Recombinant Influenza A Influenza A Virus Nucleoprotein (His tag) (AB224859)

Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS analysis result of ab224859 could indicate that this peptide derived from E.coli-expressed Influenza A virus (strain A/Beijing/353/1989 H3N2) Nucleoprotein.

Mass Spectrometry - Recombinant Influenza A Influenza A Virus Nucleoprotein (His tag) (AB224859)
  • Mass Spec

Supplier Data

Mass Spectrometry - Recombinant Influenza A Influenza A Virus Nucleoprotein (His tag) (AB224859)

Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS analysis result of ab224859 could indicate that this peptide derived from E.coli-expressed Influenza A virus (strain A/Beijing/353/1989 H3N2) Nucleoprotein.

SDS-PAGE - Recombinant Influenza A Influenza A Virus Nucleoprotein (His tag) (AB224859)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Influenza A Influenza A Virus Nucleoprotein (His tag) (AB224859)

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) analysis of ab224859 with 5% enrichment gel and 15% separation gel.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

6x His tag N-Terminus SUMO tag N-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

P69291

Animal free

No

Carrier free

No

Species

Influenza A virus (A/Beijing/353/1989(H3N2))

Storage buffer

pH: 7.2 - 7.4 Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)

storage-buffer

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Secondary Antibodies

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MASQGTKRSYEQMETDGERQNATEIRASVGKMIDGIGRFYIQMCTELKLSDYEGRLIQNSLTVERMVLSAFDERRNRYLEEHPSAGKDPKKTGGPIYKRVGGRWMRELVLYDKEEIRRIWRQANNGDDATRGLTHMMIWHSNLNDTTYQRTRALVRTGMDPRMCSLMQGSTLPRRSGAAGAAVKGIGTMVMELIRMIKRGINDRNFWRGENGRKTRSAYERMCNILKGKFQTAAQRAMMDQVRESRNPGNAEIEDLIFSARSALILRGSVAHKSCLPACVYGPAVSSGYDFEKEGYSLVGIDPFKLLQNSQVYSLIRPNENPAHKSQLVWMACHSAAFEDLRLLSFIRGTKVSPRGKLSTRGVQIASNENMDNMESSTLELRSRYWAIRTRSGGNTNQQRASAGQISVQPTFSVQRNLPFEKSTVMAAFTGNTEGRTSDMRAEIIRMMEGAKPEEVSFRGRGVFELSDEKATNPIVPSFDMSNEGSYFFGDNAEEYDN","proteinLength":"Full Length","predictedMolecularWeight":"72.2 kDa","actualMolecularWeight":null,"aminoAcidEnd":498,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":null,"tags":[{"tag":"6x His","terminus":"N-Terminus"},{"tag":"SUMO","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C|-80°C
Appropriate long-term storage conditions
-20°C|-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Influenza A Virus Nucleoprotein also known as NP or nucleoprotein influenza A plays a significant mechanical role in the virus life cycle. It is an essential structural component that encapsidates viral RNA forming ribonucleoprotein complexes important for replication and transcription. Influenza A nucleoprotein has a molecular weight of approximately 56 kDa and is expressed in infected host cells. Within the influenza virion the nucleoprotein interacts with the viral RNA and polymerase complex ensuring the integrity and functionality of the viral genome.
Biological function summary

The influenza A nucleoprotein is involved in regulating viral RNA synthesis and acts as a platform for the assembly of viral components. It does not directly form stable complexes with host proteins but it is central to the ribonucleoprotein complexes that are vital for the virus life cycle. This nucleoprotein assists in the virus's ability to hijack the host cellular machinery favoring its replication and infection processes. While the nucleoprotein itself does not form direct complexes with host proteins its interactions are significant for facilitating various stages of the viral life cycle.

Pathways

The influenza A nucleoprotein operates within the viral replication and transcription pathways. It associates with proteins such as PB1 PB2 and PA of the polymerase complex which is important for viral RNA synthesis. The nucleoprotein ensures the proper packaging of the viral genome to be recognized by the polymerase complex playing a pivotal role in the viral replication process. Through these pathways it indirectly impacts the host immune response as the virus must evade host defenses to ensure replication success.

The nucleoprotein influenza A is primarily linked to influenza infections a major respiratory illness. Influenza can manifest in a mild to severe form occasionally leading to pandemics. The nucleoprotein is an attractive target for antiviral drug development given its essential role in the viral life cycle. Moreover studies have shown that variations and mutations in the nucleoprotein can affect viral virulence and the outcome of infection. By understanding its mechanisms and interactions with other viral proteins like HA and NA researchers can better develop strategies to combat influenza outbreaks.
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Specifications

Form

Liquid

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General info

Function

Encapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the host nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals that are responsible for the active RNP import into the nucleus through cellular importin alpha/beta pathway. Later in the infection, nclear export of RNPs are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins. It is possible that nucleoprotein binds directly host exportin-1/XPO1 and plays an active role in RNPs nuclear export. M1 interaction with RNP seems to hide nucleoprotein's nuclear localization signals. Soon after a virion infects a new cell, M1 dissociates from the RNP under acidification of the virion driven by M2 protein. Dissociation of M1 from RNP unmasks nucleoprotein's nuclear localization signals, targeting the RNP to the nucleus.

Sequence similarities

Belongs to the influenza viruses nucleoprotein family.

Post-translational modifications

Late in virus-infected cells, may be cleaved from a 56-kDa protein to a 53-kDa protein by a cellular caspase. This cleavage might be a marker for the onset of apoptosis in infected cells or have a specific function in virus host interaction.

Subcellular localisation

Host nucleus

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Product protocols

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Target data

Encapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the host nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals that are responsible for the active RNP import into the nucleus through cellular importin alpha/beta pathway. Later in the infection, nclear export of RNPs are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins. It is possible that nucleoprotein binds directly host exportin-1/XPO1 and plays an active role in RNPs nuclear export. M1 interaction with RNP seems to hide nucleoprotein's nuclear localization signals. Soon after a virion infects a new cell, M1 dissociates from the RNP under acidification of the virion driven by M2 protein. Dissociation of M1 from RNP unmasks nucleoprotein's nuclear localization signals, targeting the RNP to the nucleus.
See full target information NP

Additional targets

Influenza A Virus Nucleoprotein
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Chemical biology & drug design 99:233-246 PubMed34714580

2021

Specific delivering of RNAi using Spike's aptamer-functionalized lipid nanoparticles for targeting SARS-CoV-2: A strong anti-Covid drug in a clinical case study.

Applications

Unspecified application

Species

Unspecified reactive species

Haidar Saify Nabiabad,Massoume Amini,Serwet Demirdas
View all publications
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