Recombinant Influenza A Polymerase acidic protein (His tag)
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Recombinant Influenza A Polymerase acidic protein (His tag) is a Influenza A virus (A/X-31(H3N2)) Fragment protein, in the 124 to 247 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.
View Alternative Names
Polymerase acidic protein, RNA-directed RNA polymerase subunit P2, PA
- Mass Spec
Supplier Data
Mass Spectrometry - Recombinant Influenza A Polymerase acidic protein (His tag) (AB239539)
Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS analysis result of ab239539 could indicate that this peptide derived from E.coli-expressed Influenza A virus (strain A/x-31 H3N2) Polymerase acidic protein.
- Mass Spec
Supplier Data
Mass Spectrometry - Recombinant Influenza A Polymerase acidic protein (His tag) (AB239539)
Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS analysis result of ab239539 could indicate that this peptide derived from E.coli-expressed Influenza A virus (strain A/x-31 H3N2) Polymerase acidic protein.
- SDS-PAGE
Supplier Data
SDS-PAGE - Recombinant Influenza A Polymerase acidic protein (His tag) (AB239539)
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) analysis with 5% enrichment gel and 15% separation gel of ab239539.
Reactivity data
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The polymerase acidic protein drives mRNA synthesis by cleaving host pre-mRNA and uses this as a primer for viral transcription a process often referred to as "cap-snatching". PA collaborates closely with PB1 and PB2 forming the polymerase complex that stabilizes the influenza virus's ability to propagate. This collaboration allows the virus to efficiently use host resources for synthesizing its necessary proteins and genomic RNA.
Pathways
Many research studies show that polymerase acidic protein plays a significant role in the virus's life cycle pathways. It significantly interacts with the host's transcriptional machinery during the viral replication cycle. The respiratory infection pathway where influenza virus operates depends heavily on interactions between PA PB1 and PB2 as well as between these viral proteins and host factors that facilitate viral proliferation.
Specifications
Form
Liquid
General info
Function
Plays an essential role in viral RNA transcription and replication by forming the heterotrimeric polymerase complex together with PB1 and PB2 subunits. The complex transcribes viral mRNAs by using a unique mechanism called cap-snatching. It consists in the hijacking and cleavage of host capped pre-mRNAs. These short capped RNAs are then used as primers for viral mRNAs. The PB2 subunit is responsible for the binding of the 5' cap of cellular pre-mRNAs which are subsequently cleaved after 10-13 nucleotides by the PA subunit that carries the endonuclease activity.
Sequence similarities
Belongs to the influenza viruses PA family.
Post-translational modifications
Phosphorylated on serines and threonines by host kinases, including human casein kinase II.
Subcellular localisation
Host nucleus
Target data
Complementary Products
Product promise
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