Recombinant Influenza A Polymerase acidic protein (His tag) is a Influenza A virus (A/X-31(H3N2)) Fragment protein, in the 124 to 247 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
R R E V H I Y Y L E K A N K I K S E K T H I H I F S F T G E E M A T K A D Y T L D E E S R A R I K T R L F T I R Q E M A S R G L W D S F R Q S E R G E E T I E E R F E I T G T M R K L A D Q S L P P N F S S L E N F R A Y V D G F E P N G Y I E G K L S
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Plays an essential role in viral RNA transcription and replication by forming the heterotrimeric polymerase complex together with PB1 and PB2 subunits. The complex transcribes viral mRNAs by using a unique mechanism called cap-snatching. It consists in the hijacking and cleavage of host capped pre-mRNAs. These short capped RNAs are then used as primers for viral mRNAs. The PB2 subunit is responsible for the binding of the 5' cap of cellular pre-mRNAs which are subsequently cleaved after 10-13 nucleotides by the PA subunit that carries the endonuclease activity.
Alternative names
Polymerase acidic protein, RNA-directed RNA polymerase subunit P2, PA
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Recombinant Influenza A Polymerase acidic protein (His tag) is a Influenza A virus (A/X-31(H3N2)) Fragment protein, in the 124 to 247 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- Q9IQ47-1
- Animal free
- No
- Species
- Influenza A virus (A/X-31(H3N2))
- Concentration
- Storage buffer
pH: 7.2 - 7.4
Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
- R R E V H I Y Y L E K A N K I K S E K T H I H I F S F T G E E M A T K A D Y T L D E E S R A R I K T R L F T I R Q E M A S R G L W D S F R Q S E R G E E T I E E R F E I T G T M R K L A D Q S L P P N F S S L E N F R A Y V D G F E P N G Y I E G K L S
- Accession
- Q9IQ47
- Protein length
- Fragment
- Predicted molecular weight
- 18.6 kDa
- Amino acids
- 124 to 247
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Plays an essential role in viral RNA transcription and replication by forming the heterotrimeric polymerase complex together with PB1 and PB2 subunits. The complex transcribes viral mRNAs by using a unique mechanism called cap-snatching. It consists in the hijacking and cleavage of host capped pre-mRNAs. These short capped RNAs are then used as primers for viral mRNAs. The PB2 subunit is responsible for the binding of the 5' cap of cellular pre-mRNAs which are subsequently cleaved after 10-13 nucleotides by the PA subunit that carries the endonuclease activity.
- Sequence similarities
Belongs to the influenza viruses PA family.
- Post-translational modifications
Phosphorylated on serines and threonines by host kinases, including human casein kinase II.
- Subcellular localisation
- Host nucleus
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Polymerase acidic protein also known as PA plays an important role in the transcription and replication of the influenza virus RNA genome. PA partners with PB1 and PB2 to form the influenza virus RNA polymerase complex. This heterotrimeric complex allows the virus to hijack host cell mechanisms to replicate. The polymerase acidic protein has a mass of about 83 kDa and gets expressed within nuclear compartments of infected host cells.
- Biological function summary
The polymerase acidic protein drives mRNA synthesis by cleaving host pre-mRNA and uses this as a primer for viral transcription a process often referred to as "cap-snatching". PA collaborates closely with PB1 and PB2 forming the polymerase complex that stabilizes the influenza virus's ability to propagate. This collaboration allows the virus to efficiently use host resources for synthesizing its necessary proteins and genomic RNA.
- Pathways
Many research studies show that polymerase acidic protein plays a significant role in the virus's life cycle pathways. It significantly interacts with the host's transcriptional machinery during the viral replication cycle. The respiratory infection pathway where influenza virus operates depends heavily on interactions between PA PB1 and PB2 as well as between these viral proteins and host factors that facilitate viral proliferation.
- Associated diseases and disorders
Research associates polymerase acidic protein with the pathology of influenza a highly contagious respiratory illness. The impact of PA on disease progression is related to its role in viral replication. Connections between PA and other viral proteins such as PB1 and PB2 further contribute to the virus's ability to evade immune response and cause persistent infections. Understanding PA's function and interactions can help develop antiviral strategies and influenza therapeutics.
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3 product images
SDS-PAGE - Recombinant Influenza A Polymerase acidic protein (His tag) (ab239539)
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) analysis with 5% enrichment gel and 15% separation gel of ab239539.
Mass Spectrometry - Recombinant Influenza A Polymerase acidic protein (His tag) (ab239539)
Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS analysis result of ab239539 could indicate that this peptide derived from E.coli-expressed Influenza A virus (strain A/x-31 H3N2) Polymerase acidic protein.
Mass Spectrometry - Recombinant Influenza A Polymerase acidic protein (His tag) (ab239539)
Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS analysis result of ab239539 could indicate that this peptide derived from E.coli-expressed Influenza A virus (strain A/x-31 H3N2) Polymerase acidic protein.
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