Recombinant Influenza A Virus Hemagglutinin H5 protein is a Full Length protein, in the 1 to 567 aa range, expressed in Baculovirus infected Sf9, with >80% purity and suitable for Agg, WB, SDS-PAGE.
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- Microorganisms 10:2022Transformation of by for the Expression of the Hemagglutinin of Avian Influenza Virus H5.Applications:Unspecified applicationReactive species:Unspecified reactive speciesInkar Castellanos-Huerta,Gabriela Gómez-Verduzco,Guillermo Tellez-Isaias,Guadalupe Ayora-Talavera,Bernardo Bañuelos-Hernández,Víctor Manuel Petrone-García,Gilberto Velázquez-Juárez,Isidro Fernández-SiurobPubMed 35208815
- International journal of molecular sciences 22:2021The Functional Role of Loops and Flanking Sequences of G-Quadruplex Aptamer to the Hemagglutinin of Influenza a Virus.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnastasia A Bizyaeva,Dmitry A Bunin,Valeria L Moiseenko,Alexandra S Gambaryan,Sonja Balk,Vadim N Tashlitsky,Alexander M Arutyunyan,Alexey M Kopylov,Elena G ZavyalovaPubMed 33673708
- Methods in molecular biology (Clifton, N.J.) 2132:567-5832020Preparation and Detection of Glycan-Binding Activity of Influenza Virus.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShin-Ichi Nakakita,Nongluk Sriwilaijaroen,Yasuo Suzuki,Jun HirabayashiPubMed 32306357
- Biomolecules 10:2020Structural and Functional Aspects of G-Quadruplex Aptamers Which Bind a Broad Range of Influenza A Viruses.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnastasia A Novoseltseva et. al.PubMed 31936820
Key facts
- Purity
- >80% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- Tag free
- Applications
- Agg, WB, SDS-PAGE
- Biologically active
- No
Amino acid sequence
M E K I V L L F A I V S L V K S D Q I C I G Y H A N N S T E Q V D T I M E K N V T V T H A Q D I L E K K H N G K L C D L D G V K P L I L R D C S V A G W L L G N P M C D E F I N V P E W S Y I V E K A N P V N D L C Y P G D F N D Y E E L K H L L S R I N H F E K I Q I I P K S S W S S H E A S L G V S S A C P Y Q G K S S F F R N V V W L I K K N S T Y P T I K R S Y N N T N Q E D L L V L W G I H H P N D A A E Q T K L Y Q N P T T Y I S V G T S T L N Q R L V P R I A T R S K V N G Q S G R M E F F W T I L K P N D A I N F E S N G N F I A P E Y A Y K I V K K G D S T I M K S E L E Y G N C N T K C Q T P M G A I N S S M P F H N I H P L T I G E C P K Y V K S N R L V L A T G L R N S P Q R E R R R K K R G L F G A I A G F I E G G W Q G M V D G W Y G Y H H S N E Q G S G Y A A D K E S T Q K A I D G V T N K V N S I I D K M N T Q F E A V G R E F N N L E R R I E N L N K K M E D G F L D V W T Y N A E L L V L M E N E R T L D F H D S N V K N L Y D K V R L Q L R D N A K E L G N G C F E F Y H K C D N E C M E S V R N G T Y D Y P Q Y S E E A R L K R E E I S G V K L E S I G I Y Q I L S I Y S T V A S S L A L A I M V A G L S L W M C S N G S L Q C R I C I
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application Agg | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes Quality control testing demonstrates detection of GP null under reduced conditions when using anti-H5 influenza antiserum. |
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes Because of the highly glycosylated nature of this protein, migration in an SDS-PAGE gel is slowed resulting in broad, diffuse bands representing differing glycosylation forms. |
Alternative names
Hemagglutinin 5, H5HA
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Recombinant Influenza A Virus Hemagglutinin H5 protein is a Full Length protein, in the 1 to 567 aa range, expressed in Baculovirus infected Sf9, with >80% purity and suitable for Agg, WB, SDS-PAGE.
Key facts
- Purity
- >80% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- Tag free
- Applications
- Agg, WB, SDS-PAGE
- Biologically active
- No
- Animal free
- No
- Carrier free
- Yes
- Concentration
- Storage buffer
Constituents: 99% Tris buffered saline
Sequence info
Amino acid sequence
- M E K I V L L F A I V S L V K S D Q I C I G Y H A N N S T E Q V D T I M E K N V T V T H A Q D I L E K K H N G K L C D L D G V K P L I L R D C S V A G W L L G N P M C D E F I N V P E W S Y I V E K A N P V N D L C Y P G D F N D Y E E L K H L L S R I N H F E K I Q I I P K S S W S S H E A S L G V S S A C P Y Q G K S S F F R N V V W L I K K N S T Y P T I K R S Y N N T N Q E D L L V L W G I H H P N D A A E Q T K L Y Q N P T T Y I S V G T S T L N Q R L V P R I A T R S K V N G Q S G R M E F F W T I L K P N D A I N F E S N G N F I A P E Y A Y K I V K K G D S T I M K S E L E Y G N C N T K C Q T P M G A I N S S M P F H N I H P L T I G E C P K Y V K S N R L V L A T G L R N S P Q R E R R R K K R G L F G A I A G F I E G G W Q G M V D G W Y G Y H H S N E Q G S G Y A A D K E S T Q K A I D G V T N K V N S I I D K M N T Q F E A V G R E F N N L E R R I E N L N K K M E D G F L D V W T Y N A E L L V L M E N E R T L D F H D S N V K N L Y D K V R L Q L R D N A K E L G N G C F E F Y H K C D N E C M E S V R N G T Y D Y P Q Y S E E A R L K R E E I S G V K L E S I G I Y Q I L S I Y S T V A S S L A L A I M V A G L S L W M C S N G S L Q C R I C I
- Protein length
- Full Length
- Predicted molecular weight
- 64 kDa
- Amino acids
- 1 to 567
- Nature
- Recombinant
Specifications
- Form
- Liquid
- Additional notes
Affinity purified by FPLC. Residual baculovirus GP64 co-purifies with the affinity purified ab190125 and was determined to be less the 25% of the total protein.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -80°C
- Storage information
- Avoid freeze / thaw cycle
Product promise
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3 product images
Western blot - Recombinant Influenza A Virus Hemagglutinin H5 protein (ab190125)
All lanes: anti-H5 ferret antiserum at 1/20 dilution
All lanes: Western blot - Recombinant Influenza A Virus Hemagglutinin H5 protein (ab190125) at 0.2 µg
SecondaryAll lanes: an anti-ferret AP conjugate followed by substrate.
SDS-PAGE - Recombinant Influenza A Virus Hemagglutinin H5 protein (ab190125)
SDS-PAGE analysis of 1.5 μg of ab190125 protein under reducing conditions.
Agglutination - Recombinant Influenza A Virus Hemagglutinin H5 protein (ab190125)
Hemagglutination with turkey red blood cells: ab190125 titer 1:8192
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