Recombinant Influenza A Virus Hemagglutinin H7 protein is a Full Length protein, expressed in Baculovirus infected Sf9, with >80% purity and suitable for SDS-PAGE, WB, FuncS.
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- Biomolecules 10:2020Structural and Functional Aspects of G-Quadruplex Aptamers Which Bind a Broad Range of Influenza A Viruses.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnastasia A Novoseltseva et. al.PubMed 31936820
Key facts
- Purity
- >80% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- Tag free
- Applications
- SDS-PAGE, WB, FuncS
- Biologically active
- No
Amino acid sequence
M N T Q I L V F A L I A I I P T N A D K I C L G H H A V S N G T K V N T L T E R G V E V V N A T E T V E R T N I P R I C S K G K R T V D L G Q C G L L G T I T G P P Q C D Q F L E F S A D L I I E R R E G S D V C Y P G K F V N E E A L R Q I L R E S G G I D K E A M G F T Y S G I R T N G A T S A C R R S G S S F Y A E M K W L L S N T D N A A F P Q M T K S Y K N T R K S P A L I V W G I H H S V S T A E Q T K L Y G S G N K L V T V G S S N Y Q Q S F V P S P G A R P Q V N G L S G R I D F H W L M L N P N D T V T F S F N G A F I A P D R A S F L R G K S M G I Q S G V Q V D A N C E G D C Y H S G G T I I S N L P F Q N I D S R A V G K C P R Y V K Q R S L L L A T G M K N V P E I P K G R G L F G A I A G F I E N G W E G L I D G W Y G F R H Q N A Q G E G T A A D Y K S T Q S A I D Q I T G K L N R L I E K T N Q Q F E L I D N E F N E V E K Q I G N V I N W T R D S I T E V W S Y N A E L L V A M E N Q H T I D L A D S E M D K L Y E R V K R Q L R E N A E E D G T G C F E I F H K C D D D C M A S I R N N T Y D H S K Y R E E A M Q N R I Q I D P V K L S S G Y K D V I L W F S F G A S C F I L L A I V M G L V F I C V K N G N M R C T I C I
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Alternative names
Hemagglutinin 7, HA7
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Recombinant Influenza A Virus Hemagglutinin H7 protein is a Full Length protein, expressed in Baculovirus infected Sf9, with >80% purity and suitable for SDS-PAGE, WB, FuncS.
Key facts
- Purity
- >80% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- Tag free
- Applications
- SDS-PAGE, WB, FuncS
- Biologically active
- No
- Animal free
- No
- Carrier free
- Yes
- Concentration
- Storage buffer
pH: 7.4
Constituents: 99% Tris buffered saline
Sequence info
Amino acid sequence
- M N T Q I L V F A L I A I I P T N A D K I C L G H H A V S N G T K V N T L T E R G V E V V N A T E T V E R T N I P R I C S K G K R T V D L G Q C G L L G T I T G P P Q C D Q F L E F S A D L I I E R R E G S D V C Y P G K F V N E E A L R Q I L R E S G G I D K E A M G F T Y S G I R T N G A T S A C R R S G S S F Y A E M K W L L S N T D N A A F P Q M T K S Y K N T R K S P A L I V W G I H H S V S T A E Q T K L Y G S G N K L V T V G S S N Y Q Q S F V P S P G A R P Q V N G L S G R I D F H W L M L N P N D T V T F S F N G A F I A P D R A S F L R G K S M G I Q S G V Q V D A N C E G D C Y H S G G T I I S N L P F Q N I D S R A V G K C P R Y V K Q R S L L L A T G M K N V P E I P K G R G L F G A I A G F I E N G W E G L I D G W Y G F R H Q N A Q G E G T A A D Y K S T Q S A I D Q I T G K L N R L I E K T N Q Q F E L I D N E F N E V E K Q I G N V I N W T R D S I T E V W S Y N A E L L V A M E N Q H T I D L A D S E M D K L Y E R V K R Q L R E N A E E D G T G C F E I F H K C D D D C M A S I R N N T Y D H S K Y R E E A M Q N R I Q I D P V K L S S G Y K D V I L W F S F G A S C F I L L A I V M G L V F I C V K N G N M R C T I C I
- Protein length
- Full Length
- Predicted molecular weight
- 62 kDa
- Nature
- Recombinant
Specifications
- Form
- Liquid
- Additional notes
ab190421 was purified by FPLC. Residual baculovirus GP64 co-purifies with the affinity purified Hemagglutinin GP and was determined to be less the 30% of the total protein.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
Notes
Recombinant hemagglutinin glycoprotein provides a control protein for immunoassays and a tool to enhance Orthomyxovirus research.
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3 product images
Functional Studies - Recombinant Influenza A Virus Hemagglutinin H7 protein (ab190421)
Hemagglutination with turkey red blood cells: Hemagglutinin Titer 1:2048
SDS-PAGE - Recombinant Influenza A Virus Hemagglutinin H7 protein (ab190421)
SDS-PAGE analysis of 0.4 μg ab190421 under reducing conditions.
The theoretical molecular weight of the protein is ~62 kDa, without glycosylation. Because of the highly glycosylated nature of this protein, migration in an SDS-PAGE gel is slowed resulting in broad, diffuse bands representing differing glycosylation forms.
Western blot - Recombinant Influenza A Virus Hemagglutinin H7 protein (ab190421)
Quality control testing demonstrates strong detection of GP null under reduced conditions when using anti-H7 influenza antiserum.
All lanes: Anti-H7 NY mouse antiserum at 1/100 dilution
All lanes: Western blot - Recombinant Influenza A Virus Hemagglutinin H7 protein (ab190421) at 0.38 µg
SecondaryAll lanes: Anti-mouse AP conjugate
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