Recombinant Influenza B Virus Nucleoprotein (His tag) is a Influenza B virus (B/Lee/1940) Full Length protein, in the 1 to 560 aa range, expressed in Yeast, with >90% purity and suitable for SDS-PAGE.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Yeast
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M S N M D I D S I N T G T I D K T P E E L T P G T S G A T R P I I K P A T L A P P S N K R T R N P S P E R T T T S S E T D I G R K I Q K K Q T P T E I K K S V Y K M V V K L G E F Y N Q M M V K A G L N D D M E R N L I Q N A Q A V E R I L L A A T D D K K T E Y Q K K R N A R D V K E G K E E I D H N K T G G T F Y K M V R D D K T I Y F S P I K I T F L K E E V K T M Y K T T M G S D G F S G L N H I M I G H S Q M N D V C F Q R S K G L K R V G L D P S L I S T F A G S T L P R R S G T T G V A I K G G G T L V D E A I R F I G R A M A D R G L L R D I K A K T A Y E K I L L N L K N K C S A P Q Q K A L V D Q V I G S R N P G I A D I E D L T L L A R S M V V V R P S V A S K V V L P I S I Y A K I P Q L G F N T E E Y S M V G Y E A M A L Y N M A T P V S I L R M G D D A K D K S Q L F F M S C F G A A Y E D L R V L S A L T G T E F K P R S A L K C K G F H V P A K E Q V E G M G A A L M S I K L Q F W A P M T R S G G N E V S G E G G S G Q I S C S P V F A V E R P I A L S K Q A V R R M L S M N V E G R D A D V K G N L L K M M N D S M A K K T S G N A F I G K K M F Q I S D K N K V N P I E I P I K Q T I P N F F F G R D T A E D Y D D L D Y
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Encapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the host nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals that are responsible for the active RNP import into the nucleus through cellular importin alpha/beta pathway. Later in the infection, nclear export of RNPs are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins. It is possible that nucleoprotein binds directly host exportin-1/XPO1 and plays an active role in RNPs nuclear export. M1 interaction with RNP seems to hide nucleoprotein's nuclear localization signals. Soon after a virion infects a new cell, M1 dissociates from the RNP under acidification of the virion driven by M2 protein. Dissociation of M1 from RNP unmasks nucleoprotein's nuclear localization signals, targeting the RNP to the nucleus.
Additional Targets
Influenza B Virus Nucleoprotein
Alternative names
Nucleoprotein, Nucleocapsid protein, Protein N, NP
Recommended products
Recombinant Influenza B Virus Nucleoprotein (His tag) is a Influenza B virus (B/Lee/1940) Full Length protein, in the 1 to 560 aa range, expressed in Yeast, with >90% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Yeast
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P04665-1
- Animal free
- No
- Species
- Influenza B virus (B/Lee/1940)
- Concentration
- Storage buffer
pH: 7.2 - 7.4
Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
- M S N M D I D S I N T G T I D K T P E E L T P G T S G A T R P I I K P A T L A P P S N K R T R N P S P E R T T T S S E T D I G R K I Q K K Q T P T E I K K S V Y K M V V K L G E F Y N Q M M V K A G L N D D M E R N L I Q N A Q A V E R I L L A A T D D K K T E Y Q K K R N A R D V K E G K E E I D H N K T G G T F Y K M V R D D K T I Y F S P I K I T F L K E E V K T M Y K T T M G S D G F S G L N H I M I G H S Q M N D V C F Q R S K G L K R V G L D P S L I S T F A G S T L P R R S G T T G V A I K G G G T L V D E A I R F I G R A M A D R G L L R D I K A K T A Y E K I L L N L K N K C S A P Q Q K A L V D Q V I G S R N P G I A D I E D L T L L A R S M V V V R P S V A S K V V L P I S I Y A K I P Q L G F N T E E Y S M V G Y E A M A L Y N M A T P V S I L R M G D D A K D K S Q L F F M S C F G A A Y E D L R V L S A L T G T E F K P R S A L K C K G F H V P A K E Q V E G M G A A L M S I K L Q F W A P M T R S G G N E V S G E G G S G Q I S C S P V F A V E R P I A L S K Q A V R R M L S M N V E G R D A D V K G N L L K M M N D S M A K K T S G N A F I G K K M F Q I S D K N K V N P I E I P I K Q T I P N F F F G R D T A E D Y D D L D Y
- Accession
- P04665
- Protein length
- Full Length
- Predicted molecular weight
- 63.8 kDa
- Amino acids
- 1 to 560
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Encapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the host nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals that are responsible for the active RNP import into the nucleus through cellular importin alpha/beta pathway. Later in the infection, nclear export of RNPs are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins. It is possible that nucleoprotein binds directly host exportin-1/XPO1 and plays an active role in RNPs nuclear export. M1 interaction with RNP seems to hide nucleoprotein's nuclear localization signals. Soon after a virion infects a new cell, M1 dissociates from the RNP under acidification of the virion driven by M2 protein. Dissociation of M1 from RNP unmasks nucleoprotein's nuclear localization signals, targeting the RNP to the nucleus.
- Sequence similarities
Belongs to the influenza viruses nucleoprotein family.
- Post-translational modifications
Late in virus-infected cells, may be cleaved from a 56-kDa protein to a 53-kDa protein by a cellular caspase. This cleavage might be a marker for the onset of apoptosis in infected cells or have a specific function in virus host interaction.
- Subcellular localisation
- Host nucleus
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The Influenza B Virus Nucleoprotein often known as NP or flu B NP is an essential structural protein with a molecular weight of approximately 56 kDa. It plays a role in the influenza B virus lifecycle by encapsidating the viral RNA forming the ribonucleoprotein complex important for viral replication and transcription. This protein expresses in the host cell's cytoplasm and nucleus facilitating the export of vRNPs from the nucleus.
- Biological function summary
The ribonucleoprotein complex formation involves nucleoproteins assembling with the viral RNA and polymerase subunits securing the genome for replication and transcription. NP interacts with both the polymerase basic 2 (PB2) and polymerase acidic (PA) proteins forming a vital part of the complex. This interaction allows the viral RNA to maintain its structure and function effectively during the virus infection cycle aiding both Influenza A and B viruses.
- Pathways
NP participates in the viral replication pathway supporting the influenza virus propagation in infected cells. It associates closely with viral RNA polymerases and is integral to the transcription and replication of the viral RNA genome. Other key proteins involved in these pathways include the polymerase basic 1 (PB1) protein essential for synthesizing viral mRNA and the matrix protein (M1) which aids in vRNA encapsidation and budding.
- Associated diseases and disorders
NP relates closely to influenza B infections contributing to the clinical symptoms of flu B such as fever and respiratory issues. This protein is also a potential target for vaccine and therapeutic development against influenza B. NP shares a connection with the M2 protein another target in antiviral strategies given its role in proton channel activity and viral uncoating. Understanding NP's role can guide the creation of flu B antibodies and influenza A and B antibodies to reduce disease severity or prevent flu infections.
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1 product image
SDS-PAGE - Recombinant Influenza B Virus Nucleoprotein (His tag) (ab238364)
ab238364 analyzed by (Tris-Glycine gel) discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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