Recombinant Intimin protein (His tag) is a Hafnia alvei Fragment protein, in the 1 to 280 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
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Key facts
- Purity
>90% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE
- Biologically active
No
Amino acid sequence
I T E I K A D K T T A V A N G K D A V T Y T V K V M K D G K P L S G E E V T F T T T L G T L S K S T E K T N T N G Y R K V S L T S A N Q G K S L V S A S V T M P Q L M L K L L E V E F F T Q L T I D N G N V E I V G T G A K G K L P N V W L Q Y G Q V N L K A N G G N G K Y T W Y S A N P A I A S V D P S S G Q V T L K D K G E T T I T V V S G D K Q T A I Y T I A M P N S I V S V N S S G R V D Y N T A N N I C K N I K G S L P S S I K E L K D L Y D D W G A A N K Y Q H Y S Q E S I T A W T L Q T S E N K V Q G V A S T Y D L V R K N P L I D K V D I A G N Y A Y A V C V K
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Necessary for the production of attaching and effacing lesions on tissue culture cells.
Targets
Intimin
Alternative names
Intimin, Attaching and effacing protein, Outer membrane protein, eaeA, eae
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Recombinant Intimin protein (His tag) is a Hafnia alvei Fragment protein, in the 1 to 280 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
Key facts
- Purity
>90% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE
- Biologically active
No
- Accession
- P52869-1
- Animal free
No
- Species
Hafnia alvei
- Concentration
- Storage buffer
pH: 7.2 - 7.4
Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
I T E I K A D K T T A V A N G K D A V T Y T V K V M K D G K P L S G E E V T F T T T L G T L S K S T E K T N T N G Y R K V S L T S A N Q G K S L V S A S V T M P Q L M L K L L E V E F F T Q L T I D N G N V E I V G T G A K G K L P N V W L Q Y G Q V N L K A N G G N G K Y T W Y S A N P A I A S V D P S S G Q V T L K D K G E T T I T V V S G D K Q T A I Y T I A M P N S I V S V N S S G R V D Y N T A N N I C K N I K G S L P S S I K E L K D L Y D D W G A A N K Y Q H Y S Q E S I T A W T L Q T S E N K V Q G V A S T Y D L V R K N P L I D K V D I A G N Y A Y A V C V K
- Accession
- Protein length
Fragment
- Predicted molecular weight
46.1 kDa
- Amino acids
1 to 280
- Nature
Recombinant
- Tags
His tag N-Terminus
Specifications
- Form
Liquid
General info
- Function
Necessary for the production of attaching and effacing lesions on tissue culture cells.
- Sequence similarities
Belongs to the intimin/invasin family.
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Intimin often referred to as "intimin protein" is a significant bacterial adhesion molecule involved in pathogenic processes. The intimin protein weighs approximately 94 kilodaltons and is expressed in enteropathogenic Escherichia coli (EPEC) and enterohemorrhagic Escherichia coli (EHEC). Its primary role is facilitating the intimate attachment of bacteria to the host epithelial cells contributing to bacterial colonization and infection. Intimin is classified as an outer membrane protein where it structurally integrates with the bacterial cell surface.
- Biological function summary
Intimin mediates host-microbe interactions by binding to the translocated intimin receptor (Tir) part of a type III secretion system. This interaction leads to the formation of attaching and effacing lesions a characteristic feature in infected tissues. Intimin's function is dependent on its incorporation into a supramolecular complex on the bacterial surface allowing it to engage directly with host cell receptors. By fostering tight adherence to host cells intimin enhances bacterial pathogenesis and survival in the host environment.
- Pathways
Intimin takes part in actin cytoskeleton rearrangement in host cells. This process is vital for the formation of pedestal-like structures beneath adhering bacteria. Within the Tir-intimin signaling pathway actin polymerization occurs driven by the interaction of Tir with the host proteins Nck and WASP. The actin rearrangement pathway which is important for pathogenicity highlights the cooperative role of intimin with related proteins such as Tir in modulating host cellular dynamics.
- Associated diseases and disorders
Intimin has a direct link to diarrheal diseases caused by EPEC and EHEC infections. These bacterial strains are responsible for significant gastrointestinal disorders including infantile diarrhea and hemorrhagic colitis. Intimin's interaction with the Tir protein in these bacteria is essential for pathogenesis aiding in the colonization of the intestinal tract and exacerbation of disease symptoms. Understanding the role of intimin in these disorders provides insight into potential therapeutic targets to combat these bacterial infections.
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SDS-PAGE - Recombinant Intimin protein (His tag) (ab225566)
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) analysis of ab225566 with 5% enrichment gel and 15% separation gel.
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