Recombinant Invasin protein (His tag) is a Yersinia enterocolitica Fragment protein, in the 651 to 835 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
V N G E Q F A T D K G F P K T T F N K A T F Q L V M N D D V A N N T Q Y D W T S S Y A A S A P V D N Q G K V N I A Y K T Y G S T V T V T A K S K K F P S Y T A T Y Q F K P N L W V F S G T M S L Q S S V E A S R N C Q R T D F T A L I E S A R A S N G S R S P D G T L W G E W G S L A T Y D S A E W P S G N Y W T K K T S T D F V T M D M T T G D I P T S A A T A Y P L C A E P Q
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Invasin is a protein that allows enteric bacteria to penetrate cultured mammalian cells. The entry of invasin in the cell is mediated by binding several beta-1 chain integrins.
Additional Targets
Invasin
Alternative names
Invasin
Recommended products
Recombinant Invasin protein (His tag) is a Yersinia enterocolitica Fragment protein, in the 651 to 835 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P19196-1
- Animal free
- No
- Species
- Yersinia enterocolitica
- Concentration
- Storage buffer
pH: 7.2 - 7.4
Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
- V N G E Q F A T D K G F P K T T F N K A T F Q L V M N D D V A N N T Q Y D W T S S Y A A S A P V D N Q G K V N I A Y K T Y G S T V T V T A K S K K F P S Y T A T Y Q F K P N L W V F S G T M S L Q S S V E A S R N C Q R T D F T A L I E S A R A S N G S R S P D G T L W G E W G S L A T Y D S A E W P S G N Y W T K K T S T D F V T M D M T T G D I P T S A A T A Y P L C A E P Q
- Accession
- P19196
- Protein length
- Fragment
- Predicted molecular weight
- 36.3 kDa
- Amino acids
- 651 to 835
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Invasin is a protein that allows enteric bacteria to penetrate cultured mammalian cells. The entry of invasin in the cell is mediated by binding several beta-1 chain integrins.
- Sequence similarities
Belongs to the intimin/invasin family.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Invasin is a bacterial protein that facilitates the penetration of pathogens into host cells. It weighs approximately 103 kDa and is found in the outer membrane of Yersinia species including Yersinia pseudotuberculosis and Yersinia enterocolitica. Invasin binds to host integrin receptors triggering processes that allow bacterial entry. Other names for invasin include inv and ipe reflecting its role in invasion processes. Expression of invasin occurs mainly in pathogenic bacteria when they establish infections in mammalian hosts.
- Biological function summary
Invasin mediates interactions between bacteria and host cells. It acts as a sort of molecular grappling hook by recognizing and binding to integrins which belong to the host cell's surface proteins. Through this binding invasin stimulates cytoskeletal changes which facilitate bacterial uptake. Invasin is part of a larger adhesive machinery complex and works alongside other bacterial factors to establish successful infections. Its function highlights the importance of host-pathogen interactions in bacterial pathogenesis.
- Pathways
Invasin plays a significant role in the process known as bacterial internalization into host cells. The integrin-mediated signaling pathway involves invasin binding leading to activation of kinases and cytoskeletal rearrangements. The action of invasin can influence the Rho family of small GTPases which also partake in cytoskeletal dynamics. Related proteins such as fibronectin and their integrin pairs coordinate with invasin’s activity through shared pathways to allow bacterial entry.
- Associated diseases and disorders
Invasin has been linked to gastrointestinal infections caused by Yersinia species. These infections can cause conditions such as enteritis and mesenteric lymphadenitis. Invasin's interaction with integrin receptors is critical in these disease processes. Furthermore invasin may be indirectly connected to the processes leading to autoimmunity with proteins such as HLA-DR sometimes implicated in post-infectious complications. Understanding invasin's function provides insight into bacterial invasion and potential therapeutic targets for related infections.
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1 product image
SDS-PAGE - Recombinant Invasin protein (His tag) (ab224847)
Discontinuous SDS-PAGE (Tris-Glycine gel) (reduced) analysis of ab224847 with 5% enrichment gel and 15% separation gel.
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