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AB226287

Recombinant IpaD protein (Tagged)

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Recombinant IpaD protein (Tagged) is a Shigella flexneri Full Length protein, in the 1 to 332 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE.

View Alternative Names

CP0126, ipaD, Invasin IpaD, 36 kDa membrane antigen

1 Images
SDS-PAGE - Recombinant IpaD protein (Tagged) (AB226287)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant IpaD protein (Tagged) (AB226287)

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) analysis of ab226287 with 5% enrichment gel and 15% separation gel.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

P18013

Animal free

No

Carrier free

No

Species

Shigella flexneri

Storage buffer

pH: 7.2 - 7.4 Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MNITTLTNSISTSSFSPNNTNGSSTETVNSDIKTTTSSHPVSSLTMLNDTLHNIRTTNQALKKELSQKTLTKTSLEEIALHSSQISMDVNKSAQLLDILSRNEYPINKDARELLHSAPKEAELDGDQMISHRELWAKIANSINDINEQYLKVYEHAVSSYTQMYQDFSAVLSSLAGWISPGGNDGNSVKLQVNSLKKALEELKEKYKDKPLYPANNTVSQEQANKWLTELGGTIGKVSQKNGGYVVSINMTPIDNMLKSLDNLGGNGEVVLDNAKYQAWNAGFSAEDETMKNNLQTLVQKYSNANSIFDNLVKVLSSTISSCTDTDKLFLHF","proteinLength":"Full Length","predictedMolecularWeight":"56.6 kDa","actualMolecularWeight":null,"aminoAcidEnd":332,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P18013","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IpaD also known as Invasion Plasmid Antigen D is an important protein in the type III secretion system of Shigella bacteria. It has a molecular mass of approximately 37 kDa and is important for forming the needle tip complex of this secretion system. IpaD is expressed at the bacterial surface and facilitates the translocation of effector proteins into host cells. It plays a mechanical role in assembling the secretion machinery and guiding the secretion process to target host cell membranes.
Biological function summary

The protein IpaD facilitates the effective invasion process of Shigella into epithelial cells. IpaD is a component of a complex that includes IpaB and IpaC proteins which altogether help create a translocon pore in host cell membranes. This process initiates signals that mediate bacterial entry subsequent endocytosis and evasion of host immune responses. Through this action IpaD helps maintain virulence by promoting efficient bacterial dissemination in the infected host.

Pathways

IpaD functions within the invasion signaling pathway essential for initiating infection in Shigella. This pathway involves coordination with other proteins like IpaB and IpaC which work as part of the secretion system to manipulate host cell signaling processes. IpaD is involved in pathways altering cytoskeletal dynamics through interaction with host cell proteins a necessary step in facilitating bacterial entry and spread.

IpaD has significant relevance to shigellosis an infection caused by Shigella species. The disease is characterized by inflammatory destruction of the intestinal epithelium leading to symptoms like diarrhea and abdominal pain. IpaD through its interaction with IpaB and IpaC plays a pivotal role in the pathogenicity of Shigella making it a target for developing therapeutic interventions. Understanding the interplay of these proteins contributes to potential vaccine and drug development aiming to mitigate the impact of such bacterial infections.
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Specifications

Form

Liquid

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General info

Function

Required for bacterial invasion of host cells. Controls IpaB and IpaC secretion, and the efficiency with which they are physically inserted into target cell membranes. These proteins are exported via T3SS to form a pore in the host membrane that allows the translocation of the other effectors into the host cytoplasm. Along with IpaB, is essential for both blocking secretion through the Mxi/Spa translocon in the absence of a secretion-inducing signal, and for controlling the level of secretion in the presence of this signal.

Sequence similarities

Belongs to the invasin protein D family.

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Product protocols

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Target data

Required for bacterial invasion of host cells. Controls IpaB and IpaC secretion, and the efficiency with which they are physically inserted into target cell membranes. These proteins are exported via T3SS to form a pore in the host membrane that allows the translocation of the other effectors into the host cytoplasm. Along with IpaB, is essential for both blocking secretion through the Mxi/Spa translocon in the absence of a secretion-inducing signal, and for controlling the level of secretion in the presence of this signal.
See full target information ipaD
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