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AB236802

Recombinant Limulus clotting factor c protein (Tagged)

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Recombinant Limulus clotting factor c protein (Tagged) is a Japanese Horseshoe Crab Fragment protein, in the 763 to 1019 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE.

View Alternative Names

Clotting factor C, FC, Limulus factor C

1 Images
SDS-PAGE - Recombinant Limulus clotting factor c protein (Tagged) (AB236802)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Limulus clotting factor c protein (Tagged) (AB236802)

Analysis of ab236802 by (Tris-Glycine gel) discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Key facts

Purity

>85% SDS-PAGE

Expression system

Escherichia coli

Tags

10x His tag N-Terminus Myc tag C-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

P28175

Animal free

No

Carrier free

No

Species

Japanese Horseshoe Crab

Storage buffer

pH: 7.2 - 7.4 Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"IWNGNSTEIGQWPWQAGISRWLADHNMWFLQCGGSLLNEKWIVTAAHCVTYSATAEIIDPSQFKIYLGKYYRDDSRDDDYVQVREALEIHVNPNYDPGNLNFDIALIQLKTPVTLTTRVQPICLPTDITTREHLKEGTLAVVTGWGLNENNTYSEMIQQAVLPVVAASTCEEGYKEADLPLTVTENMFCAGYKKGRYDACSGDSGGPLVFADDSRTERRWVLEGIVSWGSPSGCGKANQYGGFTKVNVFLSWIRQFI","proteinLength":"Fragment","predictedMolecularWeight":"33.8 kDa","actualMolecularWeight":null,"aminoAcidEnd":1019,"aminoAcidStart":763,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P28175","tags":[{"tag":"10x His","terminus":"N-Terminus"},{"tag":"Myc","terminus":"C-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
False
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Specifications

Form

Liquid

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General info

Function

This enzyme is closely associated with an endotoxin-sensitive hemolymph coagulation system which may play important roles in both hemostasis and host defense mechanisms (PubMed : 3512266). Its active form catalyzes the activation of clotting factor B (PubMed : 3512266).

Sequence similarities

Belongs to the peptidase S1 family.

Post-translational modifications

N-glycosylated.. Lipopolysaccharide (LPS) activates clotting factor C by inducing the proteolytic cleavage of the clotting factor C light chain into clotting factor C chains A and B (PubMed:3308457, PubMed:3512266). Clotting factor C chains heavy, A and B remain associated via interchain disulfide bonds (PubMed:3512266).

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Product protocols

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Target data

This enzyme is closely associated with an endotoxin-sensitive hemolymph coagulation system which may play important roles in both hemostasis and host defense mechanisms (PubMed : 3512266). Its active form catalyzes the activation of clotting factor B (PubMed : 3512266).
See full target information CFC_TACTR
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Product promise

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