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AB314536

Recombinant Macaca fascicularis ENPP3/B10 protein - Active

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Recombinant Macaca fascicularis ENPP3/B10 protein - Active is a Fragment protein, in the 46 to 874 aa range, expressed in Mammalian, with >95%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS.

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

Mammalian

Tags

10x His tag N-Terminus

Applications

FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

Measured by its binding ability in a functional ELISA. AB314536 at 2 μg/mL can bind an Anti-ENPP3 recombinant antibody, the EC50 is 3.313-4.724 ng/mL.

Animal free

Yes

Carrier free

No

Reconstitution

Reconstitute in water

Storage buffer

pH: 7.4 - 8 Constituents: 6% Trehalose, 2.92% Sodium chloride, 0.315% Tris

storage-buffer

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Complementary Products

Reagents, Controls & Accessories

AB325995

Prestained Protein Ladder - Broad molecular weight (11-250 kDa, 5-color, 11 bands)

SDS-PAGE - Prestained Protein Ladder - Broad molecular weight (11-250 kDa, 5-color, 11 bands) (AB325995)

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Proteins & Peptides

AB51219

Recombinant Human LAIR1 protein (Tag Free)

SDS-PAGE - Recombinant Human LAIR1 protein (Tag Free) (AB51219)

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Primary Antibodies

AB313376

Anti-ENPP3/B10 antibody [EPR27349-126]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENPP3/B10 antibody [EPR27349-126] (AB313376)

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Proteins & Peptides

AB322096

Recombinant Human SIRP alpha Protein Standard (His tag)

Sandwich ELISA - Recombinant Human SIRP alpha Protein Standard (His tag) (AB322096)

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Assay Kits

AB204722

Dipeptidyl peptidase IV (DPP4) Activity Assay Kit (Fluorometric)

Functional Studies - Dipeptidyl peptidase IV (DPP4) Activity Assay Kit (Fluorometric) (AB204722)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20degC/-80degC. Our default final concentration of glycerol is 50%.
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Sequence info

[{"sequence":"RKLEKQGSCRKKCFDASFRGLENCRCDVACEDRGDCCWDFEDTCVESTRIWTCNKFRCGETRLEASLCSCSDDCLQRKDCCADYKSVCQGETSWLEENCDTAQQSQCPEGFDLPPVILFSMDGFRAEYLYTWDTLMPNINKLKTCGIHSKYMRAMYPTKTFPNHYTIVTGLYPESHGIIDNNMYDVNLNKNFSLSSEEQNNPAWWHGQPMWLTAMYQGLKAATYFWPGSEVAINGSFPSIYMPYNRSVPYEERISTLLKWLDLPKAERPSFYTMYFEEPDSSGHASGPVSARVIKALQVVDHAFGMLMEGLKQRNLHNCVNIILLADHGMDQTYCNKMEYMTDYFPRINFYMYEGPAPRIRALNVPHDFFSFNSEEIVRNLSCRKPDQHFKPYLTPDLPKRLHYAKNVRIDKVHLFVDPQWLAVGSKSNTNCGGGNHGYNNEFRSMEAIFLAHGPSFKEKTEVEPFENIEVYNLMCDLLRIQPAPNNGTRGSLNHLLKVPFYKPSHAEEVSKFSVCGFANPLPTDNLSCLCPHLQNSIQLEQVNQMLNLTQEEITATVKVNLPFGRPRVLQKNVDNCLLYHREYVSGFGKAMRMPMWSSYTVPQLGDTSPLPPTVPDCLRADVRVPPSESQKCSFYLADENITHGFLYPPAINRTSDSQYDALIMSNLVPMYEEFRKMWDYFHSVLLIKHATERNGVNVVSGPIFDYNYDGHFDAPEEITKHIANTDIPIPTHYFVVLTSCKNKSHTPENCPGWLDVLPFIIPHRPTNVESCPEGKPEALWVEERLTAHIARVRDVELLTGLDFYQDKAQPVSEILQLKTYLPTFETTI","proteinLength":"Fragment","predictedMolecularWeight":"98.7 kDa","actualMolecularWeight":null,"aminoAcidEnd":874,"aminoAcidStart":46,"nature":"Recombinant","expressionSystem":"Mammalian","accessionNumber":null,"tags":[{"tag":"10x His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C|-80°C
Appropriate long-term storage conditions
-20°C|-80°C
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ENPP3/B10 protein also known as Ectonucleotide Pyrophosphatase/Phosphodiesterase 3 is an enzyme that plays a role in nucleotide metabolism by hydrolyzing phosphodiester and phosphosulfate bonds. This protein has a molecular mass of around 100 kDa. ENPP3 is mainly expressed on the surface of epithelial cells and you find it in tissues like the kidney liver and lung. In some contexts immune cells such as mast cells also show its expression.
Biological function summary

ENPP3 regulates the availability of extracellular nucleotides and phosphate metabolites. It is not typically considered part of a larger complex and rather contributes to various processes independently. By modulating concentrations of these extracellular molecules ENPP3 impacts cell signaling and nucleotide homeostasis which can affect cell proliferation and differentiation.

Pathways

This enzyme engages in the purinergic signaling pathway and can influence the MAPK/ERK pathway. Through its enzymatic action on nucleotides ENPP3 helps regulate signaling molecules which participate in processes such as cell growth and immune responses. Proteins related to these pathways include ATP-binding proteins like P2Y receptors and kinases involved in MAPK/ERK signaling.

ENPP3's function links it to conditions such as allergy and asthma as its expression on mast cells affects histamine release. Changes in its activity are also noted in certain cancers where its role in signaling pathways can contribute to uncontrolled cell proliferation. In these disease contexts the protein interacts with other signaling molecules like cytokine receptors and oncogenic proteins which can modulate or exacerbate disease progression.
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Specifications

Form

Lyophilized

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General info

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Product protocols

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Target data

See full target information ENPP3/B10
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Product promise

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