Recombinant Major allergen Mal d 1 protein (His tag) is a Malus domestica Full Length protein, in the 2 to 159 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
Images
Key facts
- Purity
>90% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE
- Biologically active
No
Amino acid sequence
G V Y T F E N E F T S E I P P S R L F K A F V L D A D N L I P K I A P Q A I K Q A E I L E G N G G P G T I K K I T F G E G S Q Y G Y V K H R I D S I D E A S Y S Y S Y T L I E G D A L T D T I E K I S Y E T K L V A C G S G S T I K S I S H Y H T K G N I E I K E E H V K V G K E K A H G L F K L I E S Y L K D H P D A Y N
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Alternative names
Major allergen Mal d 1, Allergen Mal d I, MALD1
Recommended products
Recombinant Major allergen Mal d 1 protein (His tag) is a Malus domestica Full Length protein, in the 2 to 159 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
Key facts
- Purity
>90% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE
- Biologically active
No
- Accession
- P43211-1
- Animal free
No
- Species
Malus domestica
- Concentration
- Storage buffer
pH: 7.2 - 7.4
Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
G V Y T F E N E F T S E I P P S R L F K A F V L D A D N L I P K I A P Q A I K Q A E I L E G N G G P G T I K K I T F G E G S Q Y G Y V K H R I D S I D E A S Y S Y S Y T L I E G D A L T D T I E K I S Y E T K L V A C G S G S T I K S I S H Y H T K G N I E I K E E H V K V G K E K A H G L F K L I E S Y L K D H P D A Y N
- Accession
- Protein length
Full Length
- Predicted molecular weight
33.5 kDa
- Amino acids
2 to 159
- Nature
Recombinant
- Tags
His tag N-Terminus
Specifications
- Form
Liquid
General info
- Sequence similarities
Belongs to the BetVI family.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Major allergen Mal d 1 also known as Mal protein or allergen d is part of the PR-10 protein family. It has a molecular weight of approximately 17 kDa. This protein is found primarily in apples specifically expressed in apple pulp and peel. As a major allergen Mal d 1 elicits allergic reactions in sensitive individuals playing an important role in the allergic response seen in apple-related oral allergy syndrome.
- Biological function summary
Major allergen Mal d 1 functions as a panallergen. It shares structural similarities with other PR-10 proteins including the Bet v 1 which is a well-known birch pollen allergen. Mal d 1 does not form a complex but has functional homology with Bet v 1 indicating a shared mechanism of action which involves acting as a carrier for hydrophobic ligands like plant hormones and fatty acids. This function may contribute to its role in plant defense although in humans it leads to immune responses such as IgE binding.
- Pathways
Major allergen Mal d 1 is involved in the signaling pathways of the plant defense system. It participates in pathways similar to those of Bet v 1 which help in regulating responses to environmental stressors and infections. Although not directly linked to any human pathways its structural similarity with Bet v 1 ties it to the IgE-mediated allergic pathway seen during an immune response to specific food allergens common in birch-allergic individuals exhibiting cross-reactivity with those allergic to apples.
- Associated diseases and disorders
Major allergen Mal d 1 is linked prominently to oral allergy syndrome. This disorder involves a reaction to certain fresh fruits and vegetables in individuals sensitive to pollen allergens like Bet v 1. In these cases immune system misidentifies Mal d 1 as Bet v 1 due to homology leading to allergic symptoms. This disorder is characterized by itching and swelling in the mouth and throat after apple consumption highlighting a clear connection between Mal d 1 and similar proteins triggering an immune response.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
SDS-PAGE - Recombinant Major allergen Mal d 1 protein (His tag) (ab226280)
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) analysis of ab226280 with 5% enrichment gel and 15% separation gel.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com