Recombinant Major pollen allergen Lol p 5a protein (Tagged) is a Lolium perenne Full Length protein, in the 26 to 307 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
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Key facts
- Purity
>90% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE
- Biologically active
No
Amino acid sequence
A D A G Y T P A A A A T P A T P A A T P A A A G G K A T T D E Q K L L E D V N A G F K A A V A A A A N A P P A D K F K I F E A A F S E S S K G L L A T S A A K A P G L I P K L D T A Y D V A Y K A A E A T P E A K Y D A F V T A L T E A L R V I A G A L E V H A V K P A T E E V L A A K I P T G E L Q I V D K I D A A F K I A A T A A N A A P T N D K F T V F E S A F N K A L N E C T G G A Y E T Y K F I P S L E A A V K Q A Y A A T V A A A P E V K Y A V F E A A L T K A I T A M T Q A Q K A G K P A A A A A T A A A T V A T A A A T A A A V L P P P L L V V Q S L I S L L I Y Y
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Alternative names
Major pollen allergen Lol p 5a, Allergen Lol p Ib, Allergen Lol p Va, LOLPIB
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Recombinant Major pollen allergen Lol p 5a protein (Tagged) is a Lolium perenne Full Length protein, in the 26 to 307 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
Key facts
- Purity
>90% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE
- Biologically active
No
- Accession
- Q40240-1
- Animal free
No
- Species
Lolium perenne
- Concentration
- Storage buffer
pH: 7.2 - 7.4
Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
A D A G Y T P A A A A T P A T P A A T P A A A G G K A T T D E Q K L L E D V N A G F K A A V A A A A N A P P A D K F K I F E A A F S E S S K G L L A T S A A K A P G L I P K L D T A Y D V A Y K A A E A T P E A K Y D A F V T A L T E A L R V I A G A L E V H A V K P A T E E V L A A K I P T G E L Q I V D K I D A A F K I A A T A A N A A P T N D K F T V F E S A F N K A L N E C T G G A Y E T Y K F I P S L E A A V K Q A Y A A T V A A A P E V K Y A V F E A A L T K A I T A M T Q A Q K A G K P A A A A A T A A A T V A T A A A T A A A V L P P P L L V V Q S L I S L L I Y Y
- Accession
- Protein length
Full Length
- Predicted molecular weight
48.4 kDa
- Amino acids
26 to 307
- Nature
Recombinant
- Tags
His tag N-Terminus
Specifications
- Form
Liquid
General info
- Sequence similarities
Belongs to the Poa p IX/Phl p VI allergen family.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The major pollen allergen Lol p 5a also known as Lolium perenne group 5 allergen is a significant protein found in ryegrass pollen. This protein weighs around 28 kDa and is widely expressed in the pollen of the perennial ryegrass a common allergenic plant. It plays a mechanical role in pollen grain wall integrity and pollen-tube growth contributing to the reproductive success of the plant. Its strong allergenic properties make it key for immune responses in individuals sensitive to grass pollen.
- Biological function summary
This allergen elicits strong immune responses in humans often leading to allergic reactions. It forms a part of a complex network of pollen proteins that trigger the body's defense mechanisms. This interaction is key in conditioning the immune system's recognition and response to non-self proteins from the pollen often causing hay fever or allergic rhinitis symptoms in many individuals. As one of the major protein components in pollen its presence is felt prominently during grass pollination seasons.
- Pathways
The immune response pathway primarily involves IgE-mediated reactions. Lol p 5a interacts with immune cells such as mast cells and basophils. It stands out in the adaptive immune pathway where these immune cells release histamines and other mediators upon activation. Other pollen proteins might compete for IgE but Lol p 5a often remains the dominant allergen prompting more targeted immune reactions.
- Associated diseases and disorders
Lol p 5a plays a notable role in allergies like hay fever and asthma conditions significantly affecting respiratory health. The allergen's interaction with immune proteins such as IgE leads to hypersensitivity reactions characterized by inflammation and nasal congestion. Its link with other pollen-derived allergenic proteins further compounds these allergic responses contributing to persistent symptoms in sensitive individuals.
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1 product image
SDS-PAGE - Recombinant Major pollen allergen Lol p 5a protein (Tagged) (ab225960)
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) analysis of ab225960 with 5% enrichment gel and 15% separation gel.
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