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AB199750

Recombinant mCherry protein (His tag)

4

(1 Review)

|

(2 Publications)

Recombinant mCherry protein (His tag) is a Full Length protein, in the 1 to 236 aa range with >97% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE and HPLC. The predicted molecular weight of ab199750 protein is 28.8 kDa.

- Save time and ensure accurate results - use our mCherry protein (His tag) protein as a control

Key facts

Purity

>97% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, HPLC

applications

Biologically active

No

Animal free

No

Carrier free

No

Reconstitution

Reconstitute at 1 mg/mL in water

Storage buffer

pH: 7.4 Constituents: 10.269% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

storage-buffer

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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mCherry ELISA Kit

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We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

Product details

Ensure the validity of your result using our recombinant mCherry protein (His tag) protein ab199750 as a control in SDS-PAGE.

Function

mCherry is the second generation monomeric red fluorescent protein that has improved brightness and photostability. The protein is suitable as a positive control for mCherry protein expression studies or as a labeling reagent. The recombinant mCherry protein ab199750 is ideal for fusion tag applications and is perfect for triple labeling with EGFP, CFP, YFP, or any other dyes.

The protein is engineered with 6xHis-tag on the N-terminus, which can be used for detection with anti-His-Tag antibody or protein purification/removal by using Ni++ beads.


Endotoxin level: <0.1 ng/µg


Check out our protein gel staining guide for SDS-PAGE here


This product is manufactured by BioVision, an Abcam company and was previously called 4993 mCherry Fluorescent Protein. 4993-100 is the same size as the 100 µg size of ab199750.

Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAEGRHSTGGMDELYK","proteinLength":"Full Length","predictedMolecularWeight":"28.8 kDa","actualMolecularWeight":null,"aminoAcidEnd":236,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":null,"tags":[{"tag":"His","terminus":"N-Terminus"}]}]

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
False

Specifications

Form

Lyophilized

Additional notes

Greater than or equal to 97% by HPLC.

General info

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Vaccines 12: PubMed39591176

2024

Vaccination Against Androgen Receptor Splice Variants to Immunologically Target Prostate Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Robert D Marek,Selena Halabi,Mu-En Wang,Jason McBane,Junping Wei,Tao Wang,Xiao Yang,Congxiao Liu,Gangjun Lei,Herbert Kim Lyerly,Ming Chen,Timothy N Trotter,Zachary C Hartman

Biochemical and biophysical research communication 516:914-920 PubMed31272717

2019

Inhibition of allogeneic islet graft rejection by VISTA-conjugated liposome.

Applications

Unspecified application

Species

Unspecified reactive species

Meng Guo,Shu Han,Yanfang Liu,Wenyuan Guo,Yuanyu Zhao,Fang Liu,Xiaomin Shi,Guoshan Ding,Quanxing Wang
View all publications

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com