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AB74559

Recombinant Measles virus Priorix, Schwarz strain, nucleocapsid protein

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(3 Publications)

Recombinant Measles virus Priorix, Schwarz strain, nucleocapsid protein is a Full Length protein, in the 1 to 525 aa range, expressed in Saccharomyces cerevisiae , with >95%, suitable for WB, ELISA, SDS-PAGE.
4 Images
Western blot - Recombinant Measles virus Priorix, Schwarz strain, nucleocapsid protein (AB74559)
  • WB

Supplier Data

Western blot - Recombinant Measles virus Priorix, Schwarz strain, nucleocapsid protein (AB74559)

SDS-PAGE and WB of yeast lysates and purified Measles Nucleoprotein (ab74559) on a 12% polyacrylamide gel. Immunoblot using measles positive human serum.

Lanes 1 - 4:

Western blot - Recombinant Measles virus Priorix, Schwarz strain, nucleocapsid protein (ab74559)

Lanes 1 - 2:

S. cerevisiae cell lysates without measles nucleoprotein (negative controls)

Lane 3:

S. cerevisiae cell lysate with recombinantly expressed measles nucleoprotein

Lane 4:

CsCl purified measles Nucleoprotein from S. cerevisiae

false

Western blot - Recombinant Measles virus Priorix, Schwarz strain, nucleocapsid protein (AB74559)
  • WB

Supplier Data

Western blot - Recombinant Measles virus Priorix, Schwarz strain, nucleocapsid protein (AB74559)

SDS-PAGE and WB of yeast lysates and purified Measles Nucleoprotein (ab74559) on a 12% polyacrylamide gel. Immunoblot using Mab against measles nucleoprotein

Lanes 1 - 4:

Western blot - Recombinant Measles virus Priorix, Schwarz strain, nucleocapsid protein (ab74559)

Lanes 1 - 2:

S. cerevisiae cell lysates without measles nucleoprotein (negative controls)

Lane 3:

S. cerevisiae cell lysate with recombinantly expressed measles nucleoprotein

Lane 4:

CsCl purified measles Nucleoprotein from S. cerevisiae

false

Western blot - Recombinant Measles virus Priorix, Schwarz strain, nucleocapsid protein (AB74559)
  • WB

Supplier Data

Western blot - Recombinant Measles virus Priorix, Schwarz strain, nucleocapsid protein (AB74559)

Lanes 1 - 4:

Western blot - Recombinant Measles virus Priorix, Schwarz strain, nucleocapsid protein (ab74559)

Lanes 1 - 2:

S. cerevisiae cell lysates without measles nucleoprotein (negative controls)

Lane 3:

S. cerevisiae cell lysate with recombinantly expressed measles nucleoprotein

Lane 4:

CsCl purified measles Nucleoprotein from S. cerevisiae

false

SDS-PAGE - Recombinant Measles virus Priorix, Schwarz strain, nucleocapsid protein (AB74559)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Measles virus Priorix, Schwarz strain, nucleocapsid protein (AB74559)

SDS-PAGE showing ab74559 at approximately 60kDa. (2 μg/lane).

Key facts

Purity

>95% SDS-PAGE

Expression system

Saccharomyces cerevisiae

Tags

Tag free

Applications

SDS-PAGE, WB, ELISA

applications

Biologically active

No

Animal free

No

Carrier free

No

Reconstitution

Reconstitute in water, 50% glycerol

Storage buffer

Constituents: PBS

storage-buffer

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1 µg/mL", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"5 µg/mL", "notes":"<p></p>" }, "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"2 µg/mL", "notes":"<p></p>" } } }

Sequence info

[{"sequence":"MATLLRSLALFKRNKDKPPITSGSGGAIRGIKHIIIVPIPGDSSITTRSRLLDRLVRLIGNPDVSGPKLTGALIGILSLFVESPGQLIQRITDDPDVSIRLLEVVQSDQSQSGLTFASRGTNMEDEADQYFSHDDPISSDQSRFGWFGNKEISDIEVQDPEGFNMILGTILAQIWVLLAKAVTAPDTAADSELRRWIKYTQQRRVVGEFRLERKWLDVVRNRIAEDLSLRRFMVALILDIKRTPGNKPRIAEMICDIDTYIVEAGLASFILTIKFGIETMYPALGLHEFAGELSTLESLMNLYQQMGETAPYMVILENSIQNKFSAGSYPLLWSYAMGVGVELENSMGGLNFGRSYFDPAYFRLGQEMVRRSAGKVSSTLASELGITAEDARLVSEIAMHTTEDKISRAVGPRQAQVSFLHGDQSENELPRLGGKEDRRVKQSRGEARESYRETGPSRASDARAAHLPTGTPLDIDTATESSQDPQDSRRSADALLRLQAMAGISEEQGSDTDTPIVYNDRNLLD","proteinLength":"Full Length","predictedMolecularWeight":"58 kDa","actualMolecularWeight":null,"aminoAcidEnd":525,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Saccharomyces cerevisiae ","accessionNumber":null,"tags":[]}]

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle|Reconstitute for long term storage
False

Specifications

Form

Lyophilized

Additional notes

ab74559 is purified by ultracentifugation.

General info

Product protocols

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Epidemiology and infection 148:e17 PubMed32014073

2020

Application of a fast and cost-effective 'three-in-one' MMR ELISA as a tool for surveying anti-MMR humoral immunity: the Hungarian experience.

Applications

Unspecified application

Species

Unspecified reactive species

K Böröcz,Z Csizmadia,Á Markovics,N Farkas,J Najbauer,T Berki,P Németh

PLoS pathogens 15:e1008064 PubMed31841557

2019

IgG3 enhances neutralization potency and Fc effector function of an HIV V2-specific broadly neutralizing antibody.

Applications

Unspecified application

Species

Unspecified reactive species

Simone I Richardson,Bronwen E Lambson,Andrew R Crowley,Arman Bashirova,Cathrine Scheepers,Nigel Garrett,Salim Abdool Karim,Nonhlanhla N Mkhize,Mary Carrington,Margaret E Ackerman,Penny L Moore,Lynn Morris

Biologicals : journal of the International Association of Biological Standardization 59:20-28 PubMed30992162

2019

Correlation between the results of two analytical methods for measuring measles virus neutralizing antibodies in source plasma and therapeutic immunoglobulin products.

Applications

Unspecified application

Species

Unspecified reactive species

Jeungwoon Hong,Daegeun Kim,Younhee Won,Jungsoon Yoon,Kuk Jin Park,Jaetaek Oh,Chan-Wha Kim
View all publications

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