Recombinant Minor nucleoprotein VP30 (His tag) is a Ebola virus - Zaire (1995) Full Length protein, in the 1 to 288 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
>90% SDS-PAGE
Escherichia coli
His tag N-Terminus
SDS-PAGE
No
M E A S Y E R G R P R A A R Q H S R D G H D H H V R A R S S S R E N Y R G E Y R Q S R S A S Q V R V P T V F H K K R V E P L T V P P A P K D I C P T L K K G F L C D S S F C K K D H Q L E S L T D R E L L L L I A R K T C G S V E Q Q L N I T A P K D S R L A N P T A D D F Q Q E E G P K I T L L T L I K T A E H W A R Q D I R T I E D S K L R A L L T L C A V M T R K F S K S Q L S L L C E T H L R R E G L G Q D Q A E P V L E V Y Q R L H S D K G G S F E A A L W Q Q W D R Q S L I M F I T A F L N I A L Q L P C E S S A V V V S G L R T L V P Q S D N E E A S T N P G T C S W S D E G T P
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Multifunctional protein that acts as a viral transcriptional activator. Promotes read-through of an RNA hairpin in the NP open reading frame to enhance viral transcription. Mechanistically, nonphosphorylated VP30 hexamers form a ternary complex with the viral leader RNA. Clamps the RNA template and the complex VP35-polymerase L together, thereby increasing the polymerase affinity for the RNA template to increase transcription initiation despite the presence of RNA secondary structures. Assists also stop-start transcription at gene junctions to promote transcription of downstream genes. Interaction with NP plays a critical role in transcription initiation by recognizing the RNA stem loop (By similarity). Interaction with host RBBP6 interferes with NP-VP30 interaction and inhibits viral RNA synthesis (PubMed:30550789). Also acts as a suppressor of RNA silencing by interacting with host DICER1 and TARBP2/TRBP (PubMed:21228243).
Transcriptional activator VP30, EbolaVP30, Minor nucleoprotein VP30, eVP30, VP30
Recombinant Minor nucleoprotein VP30 (His tag) is a Ebola virus - Zaire (1995) Full Length protein, in the 1 to 288 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
>90% SDS-PAGE
Escherichia coli
His tag N-Terminus
SDS-PAGE
No
No
Ebola virus - Zaire (1995)
pH: 7.2 - 7.4
Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)
M E A S Y E R G R P R A A R Q H S R D G H D H H V R A R S S S R E N Y R G E Y R Q S R S A S Q V R V P T V F H K K R V E P L T V P P A P K D I C P T L K K G F L C D S S F C K K D H Q L E S L T D R E L L L L I A R K T C G S V E Q Q L N I T A P K D S R L A N P T A D D F Q Q E E G P K I T L L T L I K T A E H W A R Q D I R T I E D S K L R A L L T L C A V M T R K F S K S Q L S L L C E T H L R R E G L G Q D Q A E P V L E V Y Q R L H S D K G G S F E A A L W Q Q W D R Q S L I M F I T A F L N I A L Q L P C E S S A V V V S G L R T L V P Q S D N E E A S T N P G T C S W S D E G T P
Full Length
38 kDa
1 to 288
Recombinant
His tag N-Terminus
Liquid
Multifunctional protein that acts as a viral transcriptional activator. Promotes read-through of an RNA hairpin in the NP open reading frame to enhance viral transcription. Mechanistically, nonphosphorylated VP30 hexamers form a ternary complex with the viral leader RNA. Clamps the RNA template and the complex VP35-polymerase L together, thereby increasing the polymerase affinity for the RNA template to increase transcription initiation despite the presence of RNA secondary structures. Assists also stop-start transcription at gene junctions to promote transcription of downstream genes. Interaction with NP plays a critical role in transcription initiation by recognizing the RNA stem loop (By similarity). Interaction with host RBBP6 interferes with NP-VP30 interaction and inhibits viral RNA synthesis (PubMed:30550789). Also acts as a suppressor of RNA silencing by interacting with host DICER1 and TARBP2/TRBP (PubMed:21228243).
Belongs to the filoviridae transcriptional activator VP30 family.
Phosphorylated by host. Phosphorylation negatively regulates the transcription activation. Phosphorylation and dephosphorylation take place in viral inclusion bodies and are largely influenced by the presence of NP. Dephosphorylated by host PPP2R5C; this dephosphorylation enhances viral transcription and is mediated by NP.
Blue Ice
-20°C
-20°C
Avoid freeze / thaw cycle
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel of ab238387.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com