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Recombinant Minor nucleoprotein VP30 (His tag) is a Ebola virus - Zaire (1995) Full Length protein, in the 1 to 288 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.

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Images

SDS-PAGE - Recombinant Minor nucleoprotein VP30 (His tag) (AB238387), expandable thumbnail

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

Biologically active

No

Amino acid sequence

M E A S Y E R G R P R A A R Q H S R D G H D H H V R A R S S S R E N Y R G E Y R Q S R S A S Q V R V P T V F H K K R V E P L T V P P A P K D I C P T L K K G F L C D S S F C K K D H Q L E S L T D R E L L L L I A R K T C G S V E Q Q L N I T A P K D S R L A N P T A D D F Q Q E E G P K I T L L T L I K T A E H W A R Q D I R T I E D S K L R A L L T L C A V M T R K F S K S Q L S L L C E T H L R R E G L G Q D Q A E P V L E V Y Q R L H S D K G G S F E A A L W Q Q W D R Q S L I M F I T A F L N I A L Q L P C E S S A V V V S G L R T L V P Q S D N E E A S T N P G T C S W S D E G T P

Reactivity data

Application

SDS-PAGE

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Multifunctional protein that acts as a viral transcriptional activator. Promotes read-through of an RNA hairpin in the NP open reading frame to enhance viral transcription. Mechanistically, nonphosphorylated VP30 hexamers form a ternary complex with the viral leader RNA. Clamps the RNA template and the complex VP35-polymerase L together, thereby increasing the polymerase affinity for the RNA template to increase transcription initiation despite the presence of RNA secondary structures. Assists also stop-start transcription at gene junctions to promote transcription of downstream genes. Interaction with NP plays a critical role in transcription initiation by recognizing the RNA stem loop (By similarity). Interaction with host RBBP6 interferes with NP-VP30 interaction and inhibits viral RNA synthesis (PubMed:30550789). Also acts as a suppressor of RNA silencing by interacting with host DICER1 and TARBP2/TRBP (PubMed:21228243).

Alternative names

Recommended products

Recombinant Minor nucleoprotein VP30 (His tag) is a Ebola virus - Zaire (1995) Full Length protein, in the 1 to 288 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Applications

SDS-PAGE

Accession
Q77DJ5-1
Animal free

No

Species

Ebola virus - Zaire (1995)

Concentration
Loading...
Storage buffer

pH: 7.2 - 7.4
Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)

Sequence info

Amino acid sequence

M E A S Y E R G R P R A A R Q H S R D G H D H H V R A R S S S R E N Y R G E Y R Q S R S A S Q V R V P T V F H K K R V E P L T V P P A P K D I C P T L K K G F L C D S S F C K K D H Q L E S L T D R E L L L L I A R K T C G S V E Q Q L N I T A P K D S R L A N P T A D D F Q Q E E G P K I T L L T L I K T A E H W A R Q D I R T I E D S K L R A L L T L C A V M T R K F S K S Q L S L L C E T H L R R E G L G Q D Q A E P V L E V Y Q R L H S D K G G S F E A A L W Q Q W D R Q S L I M F I T A F L N I A L Q L P C E S S A V V V S G L R T L V P Q S D N E E A S T N P G T C S W S D E G T P

Accession

Q77DJ5

Protein length

Full Length

Predicted molecular weight

38 kDa

Amino acids

1 to 288

Nature

Recombinant

Tags

His tag N-Terminus

Specifications

Form

Liquid

General info

Function

Multifunctional protein that acts as a viral transcriptional activator. Promotes read-through of an RNA hairpin in the NP open reading frame to enhance viral transcription. Mechanistically, nonphosphorylated VP30 hexamers form a ternary complex with the viral leader RNA. Clamps the RNA template and the complex VP35-polymerase L together, thereby increasing the polymerase affinity for the RNA template to increase transcription initiation despite the presence of RNA secondary structures. Assists also stop-start transcription at gene junctions to promote transcription of downstream genes. Interaction with NP plays a critical role in transcription initiation by recognizing the RNA stem loop (By similarity). Interaction with host RBBP6 interferes with NP-VP30 interaction and inhibits viral RNA synthesis (PubMed:30550789). Also acts as a suppressor of RNA silencing by interacting with host DICER1 and TARBP2/TRBP (PubMed:21228243).

Sequence similarities

Belongs to the filoviridae transcriptional activator VP30 family.

Post-translational modifications

Phosphorylated by host. Phosphorylation negatively regulates the transcription activation. Phosphorylation and dephosphorylation take place in viral inclusion bodies and are largely influenced by the presence of NP. Dephosphorylated by host PPP2R5C; this dephosphorylation enhances viral transcription and is mediated by NP.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

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1 product image

  • SDS-PAGE - Recombinant Minor nucleoprotein VP30 (His tag) (ab238387), expandable thumbnail

    SDS-PAGE - Recombinant Minor nucleoprotein VP30 (His tag) (ab238387)

    (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel of ab238387.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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