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Recombinant Mouse ACE2 protein (Fc Chimera His Tag) is a Mouse Fragment protein, in the 1 to 740 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.

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Purity

>95% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

HEK 293 cells

Tags

Fc tag C-Terminus, His tag C-Terminus

Applications

SDS-PAGE

Biologically active

No

Sequence

M S S S S W L L L S L V A V T T A Q S L T E E N A K T F L N N F N Q E A E D L S Y Q S S L A S W N Y N T N I T E E N A Q K M S E A A A K W S A F Y E E Q S K T A Q S F S L Q E I Q T P I I K R Q L Q A L Q Q S G S S A L S A D K N K Q L N T I L N T M S T I Y S T G K V C N P K N P Q E C L L L E P G L D E I M A T S T D Y N S R L W A W E G W R A E V G K Q L R P L Y E E Y V V L K N E M A R A N N Y N D Y G D Y W R G D Y E A E G A D G Y N Y N R N Q L I E D V E R T F A E I K P L Y E H L H A Y V R R K L M D T Y P S Y I S P T G C L P A H L L G D M W G R F W T N L Y P L T V P F A Q K P N I D V T D A M M N Q G W D A E R I F Q E A E K F F V S V G L P H M T Q G F W A N S M L T E P A D G R K V V C H P T A W D L G H G D F R I K M C T K V T M D N F L T A H H E M G H I Q Y D M A Y A R Q P F L L R N G A N E G F H E A V G E I M S L S A A T P K H L K S I G L L P S D F Q E D S E T E I N F L L K Q A L T I V G T L P F T Y M L E K W R W M V F R G E I P K E Q W M K K W W E M K R E I V G V V E P L P H D E T Y C D P A S L F H V S N D Y S F I R Y Y T R T I Y Q F Q F Q E A L C Q A A K Y N G S L H K C D I S N S T E A G Q K L L K M L S L G N S E P W T K A L E N V V G A R N M D V K P L L N Y F Q P L F D W L K E Q N R N S F V G W N T E W S P Y A D Q S I K V R I S L K S A L G A N A Y E W T N N E M F L F R S S V A Y A M R K Y F S I I K N Q T V P F L E E D V R V S D L K P R V S F Y F F V T S P Q N V S D V I P R S E V E D A I R M S R G R I N D V F G L N D N S L E F L G I H P T L E P P Y Q P P V T

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Shipping To:
United States

Reactivity data

Application
SDS-PAGE
Reactivity
Reacts
Dilution info
-
Notes

-

Images

Target data

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Recombinant Mouse ACE2 protein (Fc Chimera His Tag) is a Mouse Fragment protein, in the 1 to 740 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

HEK 293 cells

Applications

SDS-PAGE

Predicted molecular weight

112 kDa

Amino acids

1 to 740

Sequence

M S S S S W L L L S L V A V T T A Q S L T E E N A K T F L N N F N Q E A E D L S Y Q S S L A S W N Y N T N I T E E N A Q K M S E A A A K W S A F Y E E Q S K T A Q S F S L Q E I Q T P I I K R Q L Q A L Q Q S G S S A L S A D K N K Q L N T I L N T M S T I Y S T G K V C N P K N P Q E C L L L E P G L D E I M A T S T D Y N S R L W A W E G W R A E V G K Q L R P L Y E E Y V V L K N E M A R A N N Y N D Y G D Y W R G D Y E A E G A D G Y N Y N R N Q L I E D V E R T F A E I K P L Y E H L H A Y V R R K L M D T Y P S Y I S P T G C L P A H L L G D M W G R F W T N L Y P L T V P F A Q K P N I D V T D A M M N Q G W D A E R I F Q E A E K F F V S V G L P H M T Q G F W A N S M L T E P A D G R K V V C H P T A W D L G H G D F R I K M C T K V T M D N F L T A H H E M G H I Q Y D M A Y A R Q P F L L R N G A N E G F H E A V G E I M S L S A A T P K H L K S I G L L P S D F Q E D S E T E I N F L L K Q A L T I V G T L P F T Y M L E K W R W M V F R G E I P K E Q W M K K W W E M K R E I V G V V E P L P H D E T Y C D P A S L F H V S N D Y S F I R Y Y T R T I Y Q F Q F Q E A L C Q A A K Y N G S L H K C D I S N S T E A G Q K L L K M L S L G N S E P W T K A L E N V V G A R N M D V K P L L N Y F Q P L F D W L K E Q N R N S F V G W N T E W S P Y A D Q S I K V R I S L K S A L G A N A Y E W T N N E M F L F R S S V A Y A M R K Y F S I I K N Q T V P F L E E D V R V S D L K P R V S F Y F F V T S P Q N V S D V I P R S E V E D A I R M S R G R I N D V F G L N D N S L E F L G I H P T L E P P Y Q P P V T

Accession
Q8R0I0-1
Protein length

Fragment

Animal free

No

Nature

Recombinant

Species

Mouse

Concentration
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Storage buffer

pH: 7.5
Constituents: 0.87% Sodium chloride, 0.24% Tris, 0.01% Zinc chloride

Specifications

Form

Lyophilized

General info

Function

Essential counter-regulatory carboxypeptidase of the renin-angiotensin hormone system that is a critical regulator of blood volume, systemic vascular resistance, and thus cardiovascular homeostasis. Converts angiotensin I to angiotensin 1-9, a nine-amino acid peptide with anti-hypertrophic effects in cardiomyocytes, and angiotensin II to angiotensin 1-7, which then acts as a beneficial vasodilator and anti-proliferation agent, counterbalancing the actions of the vasoconstrictor angiotensin II. Also removes the C-terminal residue from three other vasoactive peptides, neurotensin, kinetensin, and des-Arg bradykinin, but is not active on bradykinin. Also cleaves other biological peptides, such as apelins, casomorphins and dynorphin A (By similarity). By cleavage of angiotensin II, may be an important regulator of heart function (PubMed:12075344, PubMed:12967627). By cleavage of angiotensin II, may also have a protective role in acute lung injury (PubMed:16001071). Plays an important role in amino acid transport by acting as binding partner of amino acid transporter SLC6A19, regulating its trafficking on the cell surface and its activity (PubMed:19185582, PubMed:18424768, PubMed:22677001).

Sequence similarities

Belongs to the peptidase M2 family.

Post-translational modifications

Proteolytic cleavage by ADAM17 generates a secreted form. Also cleaved by serine proteases: TMPRSS2, TMPRSS11D and HPN/TMPRSS1 (By similarity).

Storage

Shipped at conditions

Ambient - Can Ship with Ice

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

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