Recombinant Mouse ACSS2 protein (His tag) is a Mouse Full Length protein, in the 1 to 701 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
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Key facts
- Purity
>90% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE
- Biologically active
No
Amino acid sequence
M G L P E E R R K S G S G S R A R E E T G A E G R V R G W S P P P E V R R S A H V P S L Q R Y R E L H R R S V E E P R E F W G N I A K E F Y W K T A C P G P F L Q Y N F D V T K G K I F T E W M K G A T T N I C Y N V L D R N V H E K K L G D K V A F Y W E G N E P G E T T K I T Y R E L L V Q V C Q F S N V L R K Q G I Q K G D R V A I Y M P M I L E L V V A M L A C A R L G A L H S I V F A G F S A E S L C E R I L D S S C C L L I T T D A F Y R G E K L V N L K E L A D E S L E K C R E K G F P V R C C I V V K H L G R A E L G M N D S P S Q S P P V K R P C P D V Q I C W N E G V D L W W H E L M Q Q A G D E C E P E W C D A E D P L F I L Y T S G S T G K P K G V V H T I G G Y M L Y V A T T F K Y V F D F H P E D V F W C T A D I G W I T G H S Y V T Y G P L A N G A T S V L F E G I P T Y P D E G R L W S I V D K Y K V T K F Y T A P T A I R M L M K F G D D P V T K H S R A S L Q V L G T V G E P I N P E A W L W Y H R V V G S Q R C P I V D T F W Q T E T G G H M L T P L P G A T P M K P G S A S F P F F G V A P A I L N E S G E E L E G E A E G Y L V F K Q P W P G I M R T V Y G N H T R F E T T Y F K K F P G Y Y V T G D G C R R D Q D G Y Y W I T G R I D D M L N V S G H L L S T A E V E S A L V E H E A V A E A A V V G H P H P V K G E C L Y C F V T L C D G H T F S P T L T E E L K K Q I R E K I G P I A T P D Y I Q N A P G L P K T R S G K I M R R V L R K I A Q N D H D L G D T S T V A D P S V I N H L F S H R C L T T Q
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Catalyzes the synthesis of acetyl-CoA from short-chain fatty acids (PubMed:11150295, PubMed:16790548). Acetate is the preferred substrate but can also utilize propionate with a much lower affinity (PubMed:11150295).
Alternative names
Acetate--CoA ligase, Acetyl-CoA synthetase, Acetyl-CoA synthetase 1, Acyl-CoA synthetase short-chain family member 2, Acyl-activating enzyme, Propionate--CoA ligase, ACS, AceCS, AceCS1, Acss2, Acas2, Acecs1
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Recombinant Mouse ACSS2 protein (His tag) is a Mouse Full Length protein, in the 1 to 701 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
Key facts
- Purity
>90% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE
- Biologically active
No
- Accession
- Q9QXG4-1
- Animal free
No
- Species
Mouse
- Concentration
- Storage buffer
pH: 7.2 - 7.4
Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
M G L P E E R R K S G S G S R A R E E T G A E G R V R G W S P P P E V R R S A H V P S L Q R Y R E L H R R S V E E P R E F W G N I A K E F Y W K T A C P G P F L Q Y N F D V T K G K I F T E W M K G A T T N I C Y N V L D R N V H E K K L G D K V A F Y W E G N E P G E T T K I T Y R E L L V Q V C Q F S N V L R K Q G I Q K G D R V A I Y M P M I L E L V V A M L A C A R L G A L H S I V F A G F S A E S L C E R I L D S S C C L L I T T D A F Y R G E K L V N L K E L A D E S L E K C R E K G F P V R C C I V V K H L G R A E L G M N D S P S Q S P P V K R P C P D V Q I C W N E G V D L W W H E L M Q Q A G D E C E P E W C D A E D P L F I L Y T S G S T G K P K G V V H T I G G Y M L Y V A T T F K Y V F D F H P E D V F W C T A D I G W I T G H S Y V T Y G P L A N G A T S V L F E G I P T Y P D E G R L W S I V D K Y K V T K F Y T A P T A I R M L M K F G D D P V T K H S R A S L Q V L G T V G E P I N P E A W L W Y H R V V G S Q R C P I V D T F W Q T E T G G H M L T P L P G A T P M K P G S A S F P F F G V A P A I L N E S G E E L E G E A E G Y L V F K Q P W P G I M R T V Y G N H T R F E T T Y F K K F P G Y Y V T G D G C R R D Q D G Y Y W I T G R I D D M L N V S G H L L S T A E V E S A L V E H E A V A E A A V V G H P H P V K G E C L Y C F V T L C D G H T F S P T L T E E L K K Q I R E K I G P I A T P D Y I Q N A P G L P K T R S G K I M R R V L R K I A Q N D H D L G D T S T V A D P S V I N H L F S H R C L T T Q
- Accession
- Protein length
Full Length
- Predicted molecular weight
82.9 kDa
- Amino acids
1 to 701
- Nature
Recombinant
- Tags
His tag N-Terminus
Specifications
- Form
Liquid
General info
- Function
Catalyzes the synthesis of acetyl-CoA from short-chain fatty acids (PubMed:11150295, PubMed:16790548). Acetate is the preferred substrate but can also utilize propionate with a much lower affinity (PubMed:11150295).
- Sequence similarities
Belongs to the ATP-dependent AMP-binding enzyme family.
- Post-translational modifications
Reversibly acetylated at Lys-661 (PubMed:16790548). The acetyl-CoA synthase activity is inhibited by acetylation and activated by deacetylation mediated by the deacetylases SIRT1 and SIRT3.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ACSS2 also known as acyl-CoA synthetase short-chain family member 2 plays an important role in acetate metabolism and energy generation. This enzyme is responsible for the conversion of acetate into acetyl-CoA an essential metabolite in cellular processes. ACSS2 has a molecular mass of approximately 73 kDa and its expression occurs mainly in liver brain and testis tissues highlighting its widespread physiological relevance.
- Biological function summary
ACSS2 regulates cellular energy homeostasis by producing acetyl-CoA for energy production and biosynthetic reactions. This enzyme provides the necessary substrate for the synthesis of fatty acids cholesterol and acetylation of proteins impacting cellular metabolism and function. ACSS2 operates independently and does not form part of any known enzyme complex emphasizing its unique role in acetate utilization.
- Pathways
ACSS2 functions as a central component of lipid metabolism and the acetyl-CoA positioned pathways. It integrates into the glycolysis-associated pathways when it contributes to the energy-producing acetyl-CoA pool. ACSS2 interacts with other metabolic enzymes like ATP citrate lyase in controlling the balance of acetyl-CoA production which is important for both lipid synthesis and energy metabolism.
- Associated diseases and disorders
Abnormalities in ACSS2 activity have links to conditions like cancer and metabolic syndrome. Alterations in the expression or function of ACSS2 can affect energy metabolism contributing to the growth and survival of cancer cells. Additionally the protein's dysregulation might play a role in metabolic syndrome associated with improper lipid and glucose homeostasis. ACSS2 interacts with oncogenes and metabolic regulators such as AMPK emphasizing its relevance in disease modulation.
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1 product image
SDS-PAGE - Recombinant Mouse ACSS2 protein (His tag) (ab226234)
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) analysis of ab226234 with 5% enrichment gel and 15% separation gel.
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