Recombinant Mouse ADAM17 protein (His tag) is a Mouse Fragment protein, in the 18 to 563 aa range, expressed in HEK 293, with >90% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
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Key facts
- Purity
>90% SDS-PAGE
- Endotoxin level
< 1 EU/µg
- Expression system
HEK 293 cells
- Tags
His tag C-Terminus
- Applications
SDS-PAGE
- Biologically active
No
Amino acid sequence
P R P P E E A G S G S H P R L E K L D S L L S D Y D I L S L A N I Q Q H S I R K R D L Q S A T H L E T L L T F S A L K R H F K L Y L T S S T E R F S Q N L R V V V V D G K E E S E Y S V K W Q N F F S G H V V G E P D S R V L A H I G D D D V T V R I N T D G A E Y N V E P L W R F V N D T K D K R M L V Y K S E D I K D F S R L Q S P K V C G Y L N A D S E E L L P K G L I D R E P S E E F V R R V K R R A E P N P L K N T C K L L V V A D H R F Y K Y M G R G E E S T T T N Y L I E L I D R V D D I Y R N T S W D N A G F K G Y G V Q I E Q I R I L K S P Q E V K P G E R H F N M A K S F P N E E K D A W D V K M L L E Q F S F D I A E E A S K V C L A H L F T Y Q D F D M G T L G L A Y V G S P R A N S H G G V C P K A Y Y N P T V K K N I Y L N S G L T S T K N Y G K T I L T K E A D L V T T H E L G H N F G A E H D P D G L A E C A P N E D Q G G K Y V M Y P I A V S G D H E N N K M F S N C S K Q S I Y K T I E S K A Q E C F Q E R S N K V C G N S R V D E G E E C D P G I M Y L N N D T C C N S D C T L K P G V Q C S D R N S P C C K N C Q F E T A Q K K C Q E A I N A T C K G V S Y C T G N S S E C P P P G D A E D
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
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Target data
Function
Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Plays a role in the proteolytic processing of ACE2 (By similarity). Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor, MUC1 and the amyloid precursor protein (PubMed:10799547, PubMed:11108241). Acts as an activator of Notch pathway by mediating cleavage of Notch, generating the membrane-associated intermediate fragment called notch extracellular truncation (NEXT) (PubMed:10882063). Plays a role in hemostasis through shedding of GP1BA, the platelet glycoprotein Ib alpha chain (PubMed:12907434). Mediates the proteolytic cleavage of LAG3, leading to release the secreted form of LAG3 (PubMed:17245433). Mediates the proteolytic cleavage of IL6R, leading to the release of secreted form of IL6R (By similarity).
Alternative names
Disintegrin and metalloproteinase domain-containing protein 17, ADAM 17, TNF-alpha convertase, TNF-alpha-converting enzyme, Tace, Adam17
Recommended products
Recombinant Mouse ADAM17 protein (His tag) is a Mouse Fragment protein, in the 18 to 563 aa range, expressed in HEK 293, with >90% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Key facts
- Purity
>90% SDS-PAGE
- Endotoxin level
< 1 EU/µg
- Expression system
HEK 293 cells
- Tags
His tag C-Terminus
- Applications
SDS-PAGE
- Biologically active
No
- Accession
- Q9Z0F8-1
- Animal free
No
- Species
Mouse
- Reconstitution
Reconstitute at 200 µg/mL in water
- Concentration
- Storage buffer
pH: 7.4
Constituents: 5% Trehalose, 0.98% MES, 0.87% Sodium chloride
Sequence info
Amino acid sequence
P R P P E E A G S G S H P R L E K L D S L L S D Y D I L S L A N I Q Q H S I R K R D L Q S A T H L E T L L T F S A L K R H F K L Y L T S S T E R F S Q N L R V V V V D G K E E S E Y S V K W Q N F F S G H V V G E P D S R V L A H I G D D D V T V R I N T D G A E Y N V E P L W R F V N D T K D K R M L V Y K S E D I K D F S R L Q S P K V C G Y L N A D S E E L L P K G L I D R E P S E E F V R R V K R R A E P N P L K N T C K L L V V A D H R F Y K Y M G R G E E S T T T N Y L I E L I D R V D D I Y R N T S W D N A G F K G Y G V Q I E Q I R I L K S P Q E V K P G E R H F N M A K S F P N E E K D A W D V K M L L E Q F S F D I A E E A S K V C L A H L F T Y Q D F D M G T L G L A Y V G S P R A N S H G G V C P K A Y Y N P T V K K N I Y L N S G L T S T K N Y G K T I L T K E A D L V T T H E L G H N F G A E H D P D G L A E C A P N E D Q G G K Y V M Y P I A V S G D H E N N K M F S N C S K Q S I Y K T I E S K A Q E C F Q E R S N K V C G N S R V D E G E E C D P G I M Y L N N D T C C N S D C T L K P G V Q C S D R N S P C C K N C Q F E T A Q K K C Q E A I N A T C K G V S Y C T G N S S E C P P P G D A E D
- Accession
- Protein length
Fragment
- Predicted molecular weight
63.4 kDa
- Amino acids
18 to 563
- Nature
Recombinant
- Tags
His tag C-Terminus
Specifications
- Form
Lyophilized
General info
- Function
Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Plays a role in the proteolytic processing of ACE2 (By similarity). Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor, MUC1 and the amyloid precursor protein (PubMed:10799547, PubMed:11108241). Acts as an activator of Notch pathway by mediating cleavage of Notch, generating the membrane-associated intermediate fragment called notch extracellular truncation (NEXT) (PubMed:10882063). Plays a role in hemostasis through shedding of GP1BA, the platelet glycoprotein Ib alpha chain (PubMed:12907434). Mediates the proteolytic cleavage of LAG3, leading to release the secreted form of LAG3 (PubMed:17245433). Mediates the proteolytic cleavage of IL6R, leading to the release of secreted form of IL6R (By similarity).
- Post-translational modifications
The precursor is cleaved by a furin endopeptidase.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle, For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA)
Notes
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ADAM17 also known as TACE (tumor necrosis factor-alpha converting enzyme) is a metalloprotease belonging to the ADAM (a disintegrin and metalloprotease) family. This protein functions by cleaving and shedding extracellular portions of cell surface proteins. It weighs approximately 93 kDa. ADAM17 is expressed in a range of tissues including the heart liver kidney and brain. The protein plays a role in the regulation of various biological processes through the activation and inactivation of membrane-bound precursor proteins.
- Biological function summary
The ADAM17 protein regulates a multitude of cellular responses such as inflammation and growth factor signaling. It is not part of a complex but interacts with various substrates in the cell membrane. ADAM17 cleaves precursors to release soluble cytokines and growth factors like tumor necrosis factor-alpha (TNF-alpha) and epidermal growth factor receptor (EGFR) ligands which modulate cell proliferation migration and apoptosis.
- Pathways
ADAM17 plays an integral role in both the TNF and EGFR signaling pathways. These pathways are important in maintaining normal cellular homeostasis. ADAM17 interacts with proteins like TNF receptors and ligands of the EGFR family integrating signaling that affects immune response and cell growth. The regulatory function of ADAM17 in these pathways makes it an important target for modulating cellular responses triggered by external stimuli.
- Associated diseases and disorders
ADAM17 has significant implications in conditions such as rheumatoid arthritis and cancer. In rheumatoid arthritis the protein's shedding activity influences inflammatory cytokines contributing to the disease’s pathogenesis alongside proteins like TNF-alpha. In cancer ADAM17's ability to release EGFR ligands affects tumor cell proliferation and metastasis with interactions involving EGFR proteins. It is a target of therapeutic interest with research focused on developing inhibitors to manage these diseases effectively.
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1 product image
SDS-PAGE - Recombinant Mouse ADAM17 protein (His tag) (ab207138)
DTT-reduced SDS-PAGE analysis of ab207138 stained overnight with Coomassie Blue.
DTT-reduced protein migrates as 19 kDa and 20 kDa (propeptide), 55-66 kDa (mature-ADAM17) and 75-94 kDa (pro-ADAM17) in SDS-PAGE due to glycosylation.
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