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AB227417

Recombinant mouse AK2 protein (Active) (His tag N-Terminus)

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Recombinant mouse AK2 protein (Active) (His tag N-Terminus) is a Mouse Full Length protein, in the 1 to 239 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, FuncS, Mass Spec.

View Alternative Names

AK 2, ATP-AMP transphosphorylase 2, ATP:AMP phosphotransferase, Adenylate monophosphate kinase, Ak2

2 Images
SDS-PAGE - Recombinant mouse AK2 protein (Active) (His tag N-Terminus) (AB227417)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant mouse AK2 protein (Active) (His tag N-Terminus) (AB227417)

15% SDS-PAGE analysis of 3µg ab227417 .

SDS-PAGE - Recombinant mouse AK2 protein (Active) (His tag N-Terminus) (AB227417)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant mouse AK2 protein (Active) (His tag N-Terminus) (AB227417)

15% SDS-PAGE analysis of 3µg ab227417

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

FuncS, SDS-PAGE, Mass Spec

applications

Biologically active

Yes

Biological activity

Specific activity is > 40 units/mg. One unit will convert 2.0 μmoles of ADP to ATP + AMP per minute at pH 7.5 at 37°C.

Accession

Q9WTP6

Animal free

No

Carrier free

No

Species

Mouse

Storage buffer

pH: 8.5 Constituents: 10% Glycerol (glycerin, glycerine), 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Complementary Products

Proteins & Peptides

AB227415

Recombinant mouse AK 1 protein (Active) (His tag N-Terminus)

Bioactive

SDS-PAGE - Recombinant mouse AK 1 protein (Active) (His tag N-Terminus) (AB227415)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB249383

Anti-AK2 antibody [EPR11388(B)] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-AK2 antibody [EPR11388(B)] - BSA and Azide free (AB249383)

  • 0
  • 0
Secondary Antibodies

AB97180

Goat Anti-Rat IgM mu chain (HRP)

  • 5
  • 1
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSHMAPNVLASEPEIPKGIRAVLLGPPGAGKGTQAPKLAENFCVCHLATGDMLRAMVASGSELGKKLKATMDAGKLVSDEMVVELIEKNLETPSCKNGFLLDGFPRTVRQAEMLDDLMEKRKEKLDSVIEFSIQDSLLIRRITGRLIHPKSGRSYHEEFNPPKEPMKDDITGEPLIRRSDDNEKALKTRLEAYHTQTTPLVEYYRKRGIHCAIDASQTPDIVFASILAAFSKATCKDLVMFI","proteinLength":"Full Length","predictedMolecularWeight":"29.1 kDa","actualMolecularWeight":null,"aminoAcidEnd":239,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9WTP6","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The adenylate kinase 2 also known as AK2 is an enzyme that catalyzes the conversion of adenine nucleotides within the cell. It facilitates the reversible conversion of ADP into ATP and AMP playing a critical role in cellular energy homeostasis. AK2 weighing approximately 26 kDa is primarily found in the mitochondrial intermembrane space of various tissues including liver muscle and brain. Its expression allows for efficient energy transfer essential for normal mitochondrial function and cellular metabolism.
Biological function summary

AK2 functions in energy transfer and regulation in the cell serving as an important component in maintaining cellular ATP levels. AK2 is an integral part of the mitochondrial adenylate kinase system which manages energy flux in cells. By balancing the concentrations of ATP ADP and AMP AK2 directly influences cellular energy state and metabolic activity. The enzyme also supports nucleotide homeostasis therefore facilitating processes such as signal transduction and nucleic acid synthesis.

Pathways

AK2 is involved in the adenylate kinase pathway important for energy metabolism. It coordinates with other enzymes like AK1 and AK3 ensuring efficient energy distribution in the cell. AK2 also interfaces with the apoptosis signaling pathways where it plays a part in regulating programmed cell death. The interaction of AK2 within these pathways emphasizes its essential role in maintaining cellular energy balance and undergoing apoptosis when necessary.

AK2 mutations link to rare conditions like reticular dysgenesis a severe form of combined immunodeficiency. The disorder arises when AK2 fails to initiate proper energy transfer necessary for white blood cell development. AK2 is also related to mitochondrial disorders where energy production gets critically impaired manifesting in muscle weakness and neurodegenerative symptoms. The enzyme's connection to proteins involved in energy pathways suggests its potential contribution to the progression of such diseases.
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Specifications

Form

Liquid

Additional notes

ab227417 was purified using conventional chromatography techniques.

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General info

Function

Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism. Adenylate kinase activity is critical for regulation of the phosphate utilization and the AMP de novo biosynthesis pathways. Plays a key role in hematopoiesis.

Sequence similarities

Belongs to the adenylate kinase family. AK2 subfamily.

Subcellular localisation

Mitochondrion intermembrane space

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Product protocols

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Target data

Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism. Adenylate kinase activity is critical for regulation of the phosphate utilization and the AMP de novo biosynthesis pathways. Plays a key role in hematopoiesis.
See full target information Ak2
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