Recombinant mouse Alpha-synuclein protein aggregate Type 1 (Active) is a Mouse Full Length protein, in the 1 to 140 aa range with >95% purity, < 5 EU/mL endotoxin level and suitable for SDS-PAGE, Functional studies, IHC, EM and ICC. The predicted molecular weight of ab246002 protein is 14.5 kDa.
- Suitable for Thioflavin T Fluorescence assay
- Save time and ensure accurate results - use our mouse Alpha-synuclein(SNCA) protein aggregate Type 1 protein as a control
- Optimal protein bioactivity and stability
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- Cells 11:2022UBA52 Is Crucial in HSP90 Ubiquitylation and Neurodegenerative Signaling during Early Phase of Parkinson's Disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShubhangini Tiwari,Abhishek Singh,Parul Gupta,Sarika SinghPubMed 36497031
- NPJ Parkinson's disease 8:462022Cdk5 phosphorylation-induced SIRT2 nuclear translocation promotes the death of dopaminergic neurons in Parkinson's disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJianguo Yan,Pei Zhang,Jie Tan,Mao Li,Xingfeng Xu,Xiaoyun Shao,Fang Fang,Zhenyou Zou,Yali Zhou,Bo TianPubMed 35443760
- Biomolecules 12:2022Spreading of Aggregated α-Synuclein in Sagittal Organotypic Mouse Brain Slices.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBuket Uçar,Nadia Stefanova,Christian HumpelPubMed 35204664
- Toxicology letters 331:188-1992020Transfer of pathological α-synuclein from neurons to astrocytes via exosomes causes inflammatory responses after METH exposure.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYunle Meng et. al.PubMed 32569805
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 5 EU/mL
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS, IHC-P, EM, ICC
- Biologically active
- Yes
Amino acid sequence
M D V F M K G L S K A K E G V V A A A E K T K Q G V A E A A G K T K E G V L Y V G S K T K E G V V H G V T T V A E K T K E Q V T N V G G A V V T G V T A V A Q K T V E G A G N I A A A T G F V K K D Q M G K G E E G Y P Q E G I L E D M P V D P G S E A Y E M P S E E G Y Q D Y E P E A
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-P | Reactivity Reacts | Dilution info - | Notes - |
Application EM | Reactivity Reacts | Dilution info - | Notes - |
Application ICC | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
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Target data
Function
Neuronal protein that plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release (By similarity). Participates as a monomer in synaptic vesicle exocytosis by enhancing vesicle priming, fusion and dilation of exocytotic fusion pores (By similarity). Mechanistically, acts by increasing local Ca(2+) release from microdomains which is essential for the enhancement of ATP-induced exocytosis (By similarity). Acts also as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components called SNAREs (Soluble NSF Attachment Protein REceptors) at presynaptic plasma membrane in conjunction with cysteine string protein-alpha/DNAJC5 (PubMed:20798282, PubMed:25246573). This chaperone activity is important to sustain normal SNARE-complex assembly during aging (By similarity). Also plays a role in the regulation of the dopamine neurotransmission by associating with the dopamine transporter (DAT1) and thereby modulating its activity (By similarity).
Alternative names
Syn, Snca, Alpha-synuclein, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor, NACP
Recommended products
Recombinant mouse Alpha-synuclein protein aggregate Type 1 (Active) is a Mouse Full Length protein, in the 1 to 140 aa range with >95% purity, < 5 EU/mL endotoxin level and suitable for SDS-PAGE, Functional studies, IHC, EM and ICC. The predicted molecular weight of ab246002 protein is 14.5 kDa.
- Suitable for Thioflavin T Fluorescence assay
- Save time and ensure accurate results - use our mouse Alpha-synuclein(SNCA) protein aggregate Type 1 protein as a control
- Optimal protein bioactivity and stability
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 5 EU/mL
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS, IHC-P, EM, ICC
- Biologically active
- Yes
- Biological activity
- Endogenous alpha-synuclein phosphorylation. 100 μM alpha synuclein protein monomer seeded with 10 nM alpha synuclein protein PFF (ab246002) in 25 μM Thioflavin T (PBS pH 7.4, 100 μl reaction volume) generated an increased fluorescence intensity after incubation at 37°C with shaking at 600 rpm for 24 hours. Fluorescence was measured by excitation at 450 nm and emission at 485 nm on a microplate reader.
- Accession
- O55042-1
- Animal free
- No
- Carrier free
- Yes
- Species
- Mouse
- Concentration
- Storage buffer
Constituents: 95% PBS
Sequence info
Amino acid sequence
- M D V F M K G L S K A K E G V V A A A E K T K Q G V A E A A G K T K E G V L Y V G S K T K E G V V H G V T T V A E K T K E Q V T N V G G A V V T G V T A V A Q K T V E G A G N I A A A T G F V K K D Q M G K G E E G Y P Q E G I L E D M P V D P G S E A Y E M P S E E G Y Q D Y E P E A
- Accession
- O55042
- Protein length
- Full Length
- Predicted molecular weight
- 14.5 kDa
- Amino acids
- 1 to 140
- Nature
- Recombinant
Specifications
- Form
- Liquid
- Additional notes
Ion-exchange purified.
General info
- Function
Neuronal protein that plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release (By similarity). Participates as a monomer in synaptic vesicle exocytosis by enhancing vesicle priming, fusion and dilation of exocytotic fusion pores (By similarity). Mechanistically, acts by increasing local Ca(2+) release from microdomains which is essential for the enhancement of ATP-induced exocytosis (By similarity). Acts also as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components called SNAREs (Soluble NSF Attachment Protein REceptors) at presynaptic plasma membrane in conjunction with cysteine string protein-alpha/DNAJC5 (PubMed:20798282, PubMed:25246573). This chaperone activity is important to sustain normal SNARE-complex assembly during aging (By similarity). Also plays a role in the regulation of the dopamine neurotransmission by associating with the dopamine transporter (DAT1) and thereby modulating its activity (By similarity).
- Sequence similarities
Belongs to the synuclein family.
- Post-translational modifications
Phosphorylated, predominantly on serine residues. Phosphorylated on Tyr-125 upon osmotic stress.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
Ensure the validity of your result using our bioactive recombinant mouse Alpha-synuclein protein aggregate Type 1 ab246002 as a control in SDS-PAGE and western blot assays.
Check out our protein gel staining guide for SDS-PAGE here
Check out our western blot protocol for more information here
Protocol FAQ
Q: Do you have a recommended protocol for the Thioflavin T Fluorescence assay?
A: Please check our recommended Thioflavin T Fluorescence assay here
For best results, sonicate ab246002 immediately prior to use.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Alpha-synuclein often referred to by alternate names such as SNCA is a protein of around 14 kDa mass. It mainly expresses in the brain particularly in presynaptic nerve terminals. This protein functions mechanically by stabilizing synaptic vesicles and maintaining synaptic function. It exists both in soluble monomer forms and as aggregates in protein filaments. Antibodies like 4D6 and EP1536Y target monomer forms of protein for more detailed studies.
- Biological function summary
The alpha-synuclein protein plays critical roles in neuronal activity. It contributes to neurotransmitter release regulation by acting in the formation and plasticity of the presynaptic neuronal network. Alpha-synuclein doesn't usually form parts of large protein complexes but it may associate transiently with membranes and vesicular structures. The protein's monomer form has also been observed in alpha lines and related neuronal processes operating alongside various cellular functions.
- Pathways
Synaptic vesicle trafficking and dopamine neurotransmitter release are significant areas involving the alpha-synuclein protein. In these pathways alpha-synuclein interacts with other proteins like synaptophysin and protein monomer monomerizations are intrinsic to these processes. Altered function or aggregation of alpha-synuclein disrupts these pathways influencing broader neurological functions.
- Associated diseases and disorders
Alterations or accumulations of alpha-synuclein are strongly linked to Parkinson's disease and Lewy body dementia. In these conditions alpha-synuclein forms abnormal protein filaments known as Lewy bodies within neurons. These formations disrupt cellular processes and neuron health. Synucleinopathies such as these show connections with proteins like parkin and DJ-1 which also have key roles in these neurodegenerative diseases.
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6 product images
Immunocytochemistry - Recombinant mouse Alpha-synuclein protein aggregate Type 1 (Active) (ab246002)
Primary rat hippocampal neurons (DIV16) show lewy body inclusion formation and loss of cells when treated with ab246002 at 4 μg/ml (D-F) on DVI2, but not when treated with a control (A-C). Tissue: Primary hippocampal neurons. Species: Sprague-Dawley rat. Fixation: 3% formaldehyde from PFA for 20 min. Blocker: 1:1 PBS:proprietary block and 30 mL/mL of 0.1% triton-X 100 for 30 min. Primary Antibody: Mouse anti-pSer129 Antibody (1/1000) and Rabbit anti-pSer129 (1/800) for 24 hours at 4°C. Secondary Antibody: ATTO 546 Donkey Anti-Mouse (1/700) and ATTO 488 Donkey Anti-Rabbit (1/700) for 1 hour at room temperature (composite green). Counterstain: Hoechst (blue) nuclear stain at 1/3000 for 1 hour at room temperature. Localization: Lewy body incluscions. Magnification: 20x.
Immunohistochemistry (PFA fixed) - Recombinant mouse Alpha-synuclein protein aggregate Type 1 (Active) (ab246002)
Immunohistochemistry analysis of rat brain injected with ab246002. Species: Female Sprague-Dawley Rat. Rat was injected with 2μL ab246002 in each of 2 injection sites: AP+1.6, ML+2.4, DV-4.2 from skull; and AP-1.4, ML+0.2, DV-2.8 from skull. 30 days post-injection. Fixation: Saline perfusion followed by 4% PFA fixation for 48 hours. Primary antibody: rabbit monoclonal anti-pSer129 alpha synuclein. Secondary Antibody: Biotin-SP Donkey Anti-Rabbit IgG (H+L) at 1/500 for 2 hours in cold room with shaking. ABC signal amplification, DAB staining. Magnification: 20x. Alpha synuclein pathology is seen in the periform/insular cortex and the cingulate cortex on both the same (ipsi) and opposite (contra) sides as the injection sites.
Electron Microscopy - Recombinant mouse Alpha-synuclein protein aggregate Type 1 (Active) (ab246002)
TEM of ab246002. Fibrils were sonicated and image was taken at 100kx magnification.
Electron Microscopy - Recombinant mouse Alpha-synuclein protein aggregate Type 1 (Active) (ab246002)
TEM of ab246002. Image was taken at 100kx magnification.
Functional Studies - Recombinant mouse Alpha-synuclein protein aggregate Type 1 (Active) (ab246002)
ab246002 seeds the formation of new Alpha synuclein fibrils from the pool of active alpha synuclein monomers. Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures, such as those in alpha synuclein fibrils. Upon binding, the emission spectrum of the dye experiences a red-shift, and increased fluorescence intensity. Thioflavin T emission curves show increased fluorescence (correlated to alpha synuclein protein aggregation) over time when 10 nM of ab246002 is combined with 100 μM of active Alpha synuclein monomer, as compared to ab246002 and active alpha Synuclein monomer alone. Thioflavin T ex = 450 nm, em = 485 nm.
SDS-PAGE - Recombinant mouse Alpha-synuclein protein aggregate Type 1 (Active) (ab246002)
SDS-PAGE analysis of ab246002 (2 μg).
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