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AB246002

Recombinant mouse Alpha-synuclein protein aggregate Type 1 (Active)

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(5 Publications)

Recombinant mouse Alpha-synuclein protein aggregate Type 1 (Active) is a Mouse Full Length protein, in the 1 to 140 aa range with >95% purity, < 5 EU/mL endotoxin level and suitable for SDS-PAGE, Functional studies, IHC, EM and ICC. The predicted molecular weight of ab246002 protein is 14.5 kDa.

- Suitable for Thioflavin T Fluorescence assay
- Save time and ensure accurate results - use our mouse Alpha-synuclein(SNCA) protein aggregate Type 1 protein as a control
- Optimal protein bioactivity and stability

View Alternative Names

Syn, Snca, Alpha-synuclein, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor, NACP

6 Images
Electron Microscopy - Recombinant mouse Alpha-synuclein protein aggregate Type 1 (Active) (AB246002)
  • EM

Supplier Data

Electron Microscopy - Recombinant mouse Alpha-synuclein protein aggregate Type 1 (Active) (AB246002)

TEM of ab246002. Fibrils were sonicated and image was taken at 100kx magnification.

Functional Studies - Recombinant mouse Alpha-synuclein protein aggregate Type 1 (Active) (AB246002)
  • FuncS

Supplier Data

Functional Studies - Recombinant mouse Alpha-synuclein protein aggregate Type 1 (Active) (AB246002)

ab246002 seeds the formation of new Alpha synuclein fibrils from the pool of active alpha synuclein monomers. Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures, such as those in alpha synuclein fibrils. Upon binding, the emission spectrum of the dye experiences a red-shift, and increased fluorescence intensity. Thioflavin T emission curves show increased fluorescence (correlated to alpha synuclein protein aggregation) over time when 10 nM of ab246002 is combined with 100 μM of active Alpha synuclein monomer, as compared to ab246002 and active alpha Synuclein monomer alone. Thioflavin T ex = 450 nm, em = 485 nm.

Immunocytochemistry - Recombinant mouse Alpha-synuclein protein aggregate Type 1 (Active) (AB246002)
  • ICC

Supplier Data

Immunocytochemistry - Recombinant mouse Alpha-synuclein protein aggregate Type 1 (Active) (AB246002)

Primary rat hippocampal neurons (DIV16) show lewy body inclusion formation and loss of cells when treated with ab246002 at 4 μg/ml (D-F) on DVI2, but not when treated with a control (A-C). Tissue : Primary hippocampal neurons. Species : Sprague-Dawley rat. Fixation : 3% formaldehyde from PFA for 20 min. Blocker : 1 : 1 PBS : proprietary block and 30 mL/mL of 0.1% triton-X 100 for 30 min. Primary Antibody : Mouse anti-pSer129 Antibody (1/1000) and Rabbit anti-pSer129 (1/800) for 24 hours at 4°C. Secondary Antibody : ATTO 546 Donkey Anti-Mouse (1/700) and ATTO 488 Donkey Anti-Rabbit (1/700) for 1 hour at room temperature (composite green). Counterstain : Hoechst (blue) nuclear stain at 1/3000 for 1 hour at room temperature. Localization : Lewy body incluscions. Magnification : 20x.

Electron Microscopy - Recombinant mouse Alpha-synuclein protein aggregate Type 1 (Active) (AB246002)
  • EM

Supplier Data

Electron Microscopy - Recombinant mouse Alpha-synuclein protein aggregate Type 1 (Active) (AB246002)

TEM of ab246002. Image was taken at 100kx magnification.

Immunohistochemistry (PFA fixed) - Recombinant mouse Alpha-synuclein protein aggregate Type 1 (Active) (AB246002)
  • IHC (PFA fixed)

Unknown

Immunohistochemistry (PFA fixed) - Recombinant mouse Alpha-synuclein protein aggregate Type 1 (Active) (AB246002)

Immunohistochemistry analysis of rat brain injected with ab246002. Species : Female Sprague-Dawley Rat. Rat was injected with 2μL ab246002 in each of 2 injection sites : AP+1.6, ML+2.4, DV-4.2 from skull; and AP-1.4, ML+0.2, DV-2.8 from skull. 30 days post-injection. Fixation : Saline perfusion followed by 4% PFA fixation for 48 hours. Primary antibody : rabbit monoclonal anti-pSer129 alpha synuclein. Secondary Antibody : Biotin-SP Donkey Anti-Rabbit IgG (H+L) at 1/500 for 2 hours in cold room with shaking. ABC signal amplification, DAB staining. Magnification : 20x. Alpha synuclein pathology is seen in the periform/insular cortex and the cingulate cortex on both the same (ipsi) and opposite (contra) sides as the injection sites.

SDS-PAGE - Recombinant mouse Alpha-synuclein protein aggregate Type 1 (Active) (AB246002)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant mouse Alpha-synuclein protein aggregate Type 1 (Active) (AB246002)

SDS-PAGE analysis of ab246002 (2 μg).

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

< 5 EU/mL

Expression system

Escherichia coli

Tags

Tag free

Applications

IHC-P, SDS-PAGE, ICC, WB, EM, FuncS

applications

Biologically active

Yes

Biological activity

Endogenous alpha-synuclein phosphorylation. 100 μM alpha synuclein protein monomer seeded with 10 nM alpha synuclein protein PFF (ab246002) in 25 μM Thioflavin T (PBS pH 7.4, 100 μl reaction volume) generated an increased fluorescence intensity after incubation at 37°C with shaking at 600 rpm for 24 hours. Fluorescence was measured by excitation at 450 nm and emission at 485 nm on a microplate reader.

Accession

O55042

Animal free

No

Carrier free

Yes

Species

Mouse

Storage buffer

pH: 7.4 Constituents: PBS

storage-buffer

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Complementary Products

Primary Antibodies

AB209538

Anti-Alpha-synuclein aggregate antibody [MJFR-14-6-4-2] - Conformation-Specific

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Alpha-synuclein aggregate antibody [MJFR-14-6-4-2] - Conformation-Specific (AB209538)

  • 4
  • 6
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

  • 5
  • 230
Assay Kits

AB282865

Mouse Alpha-synuclein ELISA Kit

Recombinant|SimpleStep

ELISA - Mouse Alpha-synuclein ELISA Kit (AB282865)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "EM": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ICC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Ensure the validity of your result using our bioactive recombinant mouse Alpha-synuclein protein aggregate Type 1 ab246002 as a control in SDS-PAGE and western blot assays.

Check out our protein gel staining guide for SDS-PAGE here

Check out our western blot protocol for more information here


Protocol FAQ

Q: Do you have a recommended protocol for the Thioflavin T Fluorescence assay?

A: Please check our recommended Thioflavin T Fluorescence assay here


For best results, sonicate ab246002 immediately prior to use.
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Sequence info

[{"sequence":"MDVFMKGLSKAKEGVVAAAEKTKQGVAEAAGKTKEGVLYVGSKTKEGVVHGVTTVAEKTKEQVTNVGGAVVTGVTAVAQKTVEGAGNIAAATGFVKKDQMGKGEEGYPQEGILEDMPVDPGSEAYEMPSEEGYQDYEPEA","proteinLength":"Full Length","predictedMolecularWeight":"14.5 kDa","actualMolecularWeight":null,"aminoAcidEnd":140,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"O55042","tags":[]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha-synuclein often referred to by alternate names such as SNCA is a protein of around 14 kDa mass. It mainly expresses in the brain particularly in presynaptic nerve terminals. This protein functions mechanically by stabilizing synaptic vesicles and maintaining synaptic function. It exists both in soluble monomer forms and as aggregates in protein filaments. Antibodies like 4D6 and EP1536Y target monomer forms of protein for more detailed studies.
Biological function summary

The alpha-synuclein protein plays critical roles in neuronal activity. It contributes to neurotransmitter release regulation by acting in the formation and plasticity of the presynaptic neuronal network. Alpha-synuclein doesn't usually form parts of large protein complexes but it may associate transiently with membranes and vesicular structures. The protein's monomer form has also been observed in alpha lines and related neuronal processes operating alongside various cellular functions.

Pathways

Synaptic vesicle trafficking and dopamine neurotransmitter release are significant areas involving the alpha-synuclein protein. In these pathways alpha-synuclein interacts with other proteins like synaptophysin and protein monomer monomerizations are intrinsic to these processes. Altered function or aggregation of alpha-synuclein disrupts these pathways influencing broader neurological functions.

Alterations or accumulations of alpha-synuclein are strongly linked to Parkinson's disease and Lewy body dementia. In these conditions alpha-synuclein forms abnormal protein filaments known as Lewy bodies within neurons. These formations disrupt cellular processes and neuron health. Synucleinopathies such as these show connections with proteins like parkin and DJ-1 which also have key roles in these neurodegenerative diseases.
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Specifications

Form

Liquid

Additional notes

Ion-exchange purified.

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General info

Function

Neuronal protein that plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release (By similarity). Participates as a monomer in synaptic vesicle exocytosis by enhancing vesicle priming, fusion and dilation of exocytotic fusion pores (By similarity). Mechanistically, acts by increasing local Ca(2+) release from microdomains which is essential for the enhancement of ATP-induced exocytosis (By similarity). Also acts as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components called SNAREs (Soluble NSF Attachment Protein REceptors) at presynaptic plasma membrane in conjunction with cysteine string protein-alpha/DNAJC5 (PubMed : 20798282, PubMed : 25246573). This chaperone activity is important to sustain normal SNARE-complex assembly during aging (By similarity). Also plays a role in the regulation of the dopamine neurotransmission by associating with the dopamine transporter (DAT1) and thereby modulating its activity (By similarity).

Sequence similarities

Belongs to the synuclein family.

Post-translational modifications

Phosphorylated, predominantly on serine residues. Phosphorylated on Tyr-125 upon osmotic stress.. Ubiquitinated. The predominant conjugate is the diubiquitinated form.. Acetylation at Met-1 seems to be important for proper folding and native oligomeric structure.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Neuronal protein that plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release (By similarity). Participates as a monomer in synaptic vesicle exocytosis by enhancing vesicle priming, fusion and dilation of exocytotic fusion pores (By similarity). Mechanistically, acts by increasing local Ca(2+) release from microdomains which is essential for the enhancement of ATP-induced exocytosis (By similarity). Also acts as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components called SNAREs (Soluble NSF Attachment Protein REceptors) at presynaptic plasma membrane in conjunction with cysteine string protein-alpha/DNAJC5 (PubMed : 20798282, PubMed : 25246573). This chaperone activity is important to sustain normal SNARE-complex assembly during aging (By similarity). Also plays a role in the regulation of the dopamine neurotransmission by associating with the dopamine transporter (DAT1) and thereby modulating its activity (By similarity).
See full target information Snca
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Cell biology and toxicology 41:34 PubMed39847159

2025

Manganese exposure induces parkinsonism-like symptoms by Serpina3n-TFEB-v/p-ATPase signaling mediated lysosomal dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Huihui Hong,Sicheng Liu,Ting Yang,Jinxian Lin,Kun Luo,Yudong Xu,Ting Li,Yu Xi,Lingling Yang,Yuan-Qiang Lu,Wei Yuan,Zhou Zhou

Cells 11: PubMed36497031

2022

UBA52 Is Crucial in HSP90 Ubiquitylation and Neurodegenerative Signaling during Early Phase of Parkinson's Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Shubhangini Tiwari,Abhishek Singh,Parul Gupta,Sarika Singh

NPJ Parkinson's disease 8:46 PubMed35443760

2022

Cdk5 phosphorylation-induced SIRT2 nuclear translocation promotes the death of dopaminergic neurons in Parkinson's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Jianguo Yan,Pei Zhang,Jie Tan,Mao Li,Xingfeng Xu,Xiaoyun Shao,Fang Fang,Zhenyou Zou,Yali Zhou,Bo Tian

Biomolecules 12: PubMed35204664

2022

Spreading of Aggregated α-Synuclein in Sagittal Organotypic Mouse Brain Slices.

Applications

Unspecified application

Species

Unspecified reactive species

Buket Uçar,Nadia Stefanova,Christian Humpel

Toxicology letters 331:188-199 PubMed32569805

2020

Transfer of pathological α-synuclein from neurons to astrocytes via exosomes causes inflammatory responses after METH exposure.

Applications

Unspecified application

Species

Unspecified reactive species

Yunle Meng,Jiuyang Ding,Chen Li,Haoliang Fan,Yitong He,Pingming Qiu
View all publications
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