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Recombinant mouse Alpha-synuclein protein aggregate Type 1 (Active) is a Mouse Full Length protein, in the 1 to 140 aa range, expressed in Escherichia coli, with >95% purity, < 5 EU/mL endotoxin level and suitable for SDS-PAGE, FuncS, IHC-P, EM, ICC.

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Purity

>95% SDS-PAGE

Endotoxin level

< 5 EU/mL

Expression system

Escherichia coli

Tags

Tag free

Applications

SDS-PAGE, FuncS, IHC-P, EM, ICC

Biologically active

Yes

Sequence

M D V F M K G L S K A K E G V V A A A E K T K Q G V A E A A G K T K E G V L Y V G S K T K E G V V H G V T T V A E K T K E Q V T N V G G A V V T G V T A V A Q K T V E G A G N I A A A T G F V K K D Q M G K G E E G Y P Q E G I L E D M P V D P G S E A Y E M P S E E G Y Q D Y E P E A

Reactivity data

Application
SDS-PAGE
Reactivity
Reacts
Dilution info
-
Notes

-

Application
FuncS
Reactivity
Reacts
Dilution info
-
Notes

-

Application
IHC-P
Reactivity
Reacts
Dilution info
-
Notes

-

Application
EM
Reactivity
Reacts
Dilution info
-
Notes

-

Application
ICC
Reactivity
Reacts
Dilution info
-
Notes

-

Images

Target data

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Recombinant mouse Alpha-synuclein protein aggregate Type 1 (Active) is a Mouse Full Length protein, in the 1 to 140 aa range, expressed in Escherichia coli, with >95% purity, < 5 EU/mL endotoxin level and suitable for SDS-PAGE, FuncS, IHC-P, EM, ICC.

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

< 5 EU/mL

Expression system

Escherichia coli

Applications

SDS-PAGE, FuncS, IHC-P, EM, ICC

Biological activity

Endogenous alpha-synuclein phosphorylation. 100 μM alpha synuclein protein monomer seeded with 10 nM alpha synuclein protein PFF (ab246002) in 25 μM Thioflavin T (PBS pH 7.4, 100 μl reaction volume) generated an increased fluorescence intensity after incubation at 37°C with shaking at 600 rpm for 24 hours. Fluorescence was measured by excitation at 450 nm and emission at 485 nm on a microplate reader.

Predicted molecular weight

14.5 kDa

Amino acids

1 to 140

Sequence

M D V F M K G L S K A K E G V V A A A E K T K Q G V A E A A G K T K E G V L Y V G S K T K E G V V H G V T T V A E K T K E Q V T N V G G A V V T G V T A V A Q K T V E G A G N I A A A T G F V K K D Q M G K G E E G Y P Q E G I L E D M P V D P G S E A Y E M P S E E G Y Q D Y E P E A

Accession
O55042-1
Protein length

Full Length

Animal free

No

Carrier free

Yes

Nature

Recombinant

Species

Mouse

Concentration
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Storage buffer

Constituents: 95% PBS

Specifications

Form

Liquid

Additional notes

Ion-exchange purified.

General info

Function

Neuronal protein that plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release. Participates as a monomer in synaptic vesicle exocytosis by enhancing vesicle priming, fusion and dilation of exocytotic fusion pores. Mechanistically, acts by increasing local Ca(2+) release from microdomains which is essential for the enhancement of ATP-induced exocytosis. Acts also as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components called SNAREs (Soluble NSF Attachment Protein REceptors) at presynaptic plasma membrane in conjunction with cysteine string protein-alpha/DNAJC5 (PubMed:20798282, PubMed:25246573). This chaperone activity is important to sustain normal SNARE-complex assembly during aging. Plays also a role in the regulation of the dopamine neurotransmission by associating with the dopamine transporter (DAT1) and thereby modulating its activity (By similarity).

Sequence similarities

Belongs to the synuclein family.

Post-translational modifications

Phosphorylated, predominantly on serine residues. Phosphorylated on Tyr-125 upon osmotic stress (By similarity).

Subcellular localisation

Nucleus

Storage

Shipped at conditions

Dry Ice

Appropriate long-term storage conditions

-80°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

This product is an active protein and may elicit a biological response in vivo, handle with caution.

Notes

Active Mouse recombinant Alpha Synuclein Pre-Formed Fibrils (Type 1).

For best results, sonicate immediately prior to use.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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