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AB316578

Recombinant Mouse ALT Protein Standard

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Recombinant Mouse ALT Protein Standard is a Mouse Fragment protein, expressed in Escherichia coli, with >80%, suitable for SDS-PAGE, sELISA.

View Alternative Names

Gpt1, Gpt, Alanine aminotransferase 1, ALT1, Glutamate pyruvate transaminase 1, Glutamic--alanine transaminase 1, Glutamic--pyruvic transaminase 1, GPT 1

2 Images
Sandwich ELISA - Recombinant Mouse ALT Protein Standard (AB316578)
  • sELISA

Supplier Data

Sandwich ELISA - Recombinant Mouse ALT Protein Standard (AB316578)

Sandwich ELISA with the capture antibody dilution at 2 μg/mL and detector antibody dilution at 0.5 μg/mL.

SDS-PAGE - Recombinant Mouse ALT Protein Standard (AB316578)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Mouse ALT Protein Standard (AB316578)

SDS-PAGE analysis of ab316578 under reducing conditions for 2ug protein

Key facts

Purity

>80% SDS-PAGE

Expression system

Escherichia coli

Tags

Tag free

Applications

sELISA, SDS-PAGE

applications

Biologically active

No

Accession

Q8QZR5

Animal free

Yes

Carrier free

No

Species

Mouse

Storage buffer

pH: 7.3 - 7.5 Constituents: 2.922% Sodium chloride, 0.64107% disodium;hydrogen phosphate;dodecahydrate, 0.02858% Potassium phosphate monobasic

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Complementary Products

Reagents, Controls & Accessories

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Proteins & Peptides

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Recombinant Human GPT2 protein (His tag N-Terminus)

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

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Assay Kits

AB234579

Rat ALT ELISA Kit

Recombinant|SimpleStep

ELISA - Rat ALT ELISA Kit (AB234579)

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Assay Kits

AB241035

ALT Assay Kit (384 well, Colorimetric/Fluorometric)

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  • 5
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

While the standard is the same as the one provided in the corresponding SimpleStep ELISA Kit, it cannot be treated as the consumable provided with our SimpleStep ELISA Kit due to differences in its concentration calibration.

Abcam guarantee that this protein standard is suitable for use in a sandwich ELISA. Individual results may vary due to differences in technique, laboratory equipment, buffers, and other experimental factors. The detection range provided for this protein standard is based on initial sandwich ELISA validation data.

The protein concentration is the concentration after validation on our sandwich ELISA platform. This Standard protein is guaranteed to work with our Capture and Detector antibodies in sELISA. Please contact our Scientific Support team to know which antibody pair is suitable for this protein.

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Sequence info

[{"sequence":null,"proteinLength":"Fragment","predictedMolecularWeight":"55.5 kDa","actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q8QZR5","tags":[]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Specifications

Form

Liquid

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General info

Function

Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles (By similarity).

Sequence similarities

Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Alanine aminotransferase subfamily.

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Product protocols

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Target data

Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles (By similarity).
See full target information Gpt
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com