Recombinant Mouse Angiopoietin-like 4/ANGPTL4 protein (His tag) is a Mouse Fragment protein, in the 167 to 410 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
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Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
K R L P K M T Q L I G L T P N A T H L H R P P R D C Q E L F Q E G E R H S G L F Q I Q P L G S P P F L V N C E M T S D G G W T V I Q R R L N G S V D F N Q S W E A Y K D G F G D P Q G E F W L G L E K M H S I T G N R G S Q L A V Q L Q D W D G N A K L L Q F P I H L G G E D T A Y S L Q L T E P T A N E L G A T N V S P N G L S L P F S T W D Q D H D L R G D L N C A K S L S G G W W F G T C S H S N L N G Q Y F H S I P R Q R Q E R K K G I F W K T W K G R Y Y P L Q A T T L L I Q P M E A T A A S
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Mediates inactivation of the lipoprotein lipase LPL, and thereby plays a role in the regulation of triglyceride clearance from the blood serum and in lipid metabolism (PubMed:15837923, PubMed:17609370, PubMed:29899519). May also play a role in regulating glucose homeostasis and insulin sensitivity (PubMed:15837923, PubMed:29899519). Inhibits proliferation, migration, and tubule formation of endothelial cells and reduces vascular leakage (PubMed:14583458, PubMed:17130448, PubMed:21832056). Upon heterologous expression, inhibits the adhesion of endothelial cell to the extracellular matrix (ECM), and inhibits the reorganization of the actin cytoskeleton, formation of actin stress fibers and focal adhesions in endothelial cells that have adhered to ANGPTL4-containing ECM (in vitro) (By similarity). Depending on context, may modulate tumor-related angiogenesis (Probable). ANGPTL4 N-terminal chain. Mediates inactivation of the lipoprotein lipase LPL, and thereby plays an important role in the regulation of triglyceride clearance from the blood serum and in lipid metabolism. Has higher activity in LPL inactivation than the uncleaved protein.
Alternative names
Farp, Fiaf, Ng27, Angptl4, Angiopoietin-related protein 4, 425O18-1, Angiopoietin-like protein 4, Fasting-induced adipose factor, Hepatic fibrinogen/angiopoietin-related protein, Secreted protein Bk89, HFARP
Recommended products
Recombinant Mouse Angiopoietin-like 4/ANGPTL4 protein (His tag) is a Mouse Fragment protein, in the 167 to 410 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- Q9Z1P8-1
- Animal free
- No
- Species
- Mouse
- Concentration
- Storage buffer
pH: 7.4
Constituents: 100% PBS
Sequence info
Amino acid sequence
- K R L P K M T Q L I G L T P N A T H L H R P P R D C Q E L F Q E G E R H S G L F Q I Q P L G S P P F L V N C E M T S D G G W T V I Q R R L N G S V D F N Q S W E A Y K D G F G D P Q G E F W L G L E K M H S I T G N R G S Q L A V Q L Q D W D G N A K L L Q F P I H L G G E D T A Y S L Q L T E P T A N E L G A T N V S P N G L S L P F S T W D Q D H D L R G D L N C A K S L S G G W W F G T C S H S N L N G Q Y F H S I P R Q R Q E R K K G I F W K T W K G R Y Y P L Q A T T L L I Q P M E A T A A S
- Accession
- Q9Z1P8
- Protein length
- Fragment
- Predicted molecular weight
- 29.9 kDa
- Amino acids
- 167 to 410
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Lyophilized
General info
- Function
Mediates inactivation of the lipoprotein lipase LPL, and thereby plays a role in the regulation of triglyceride clearance from the blood serum and in lipid metabolism (PubMed:15837923, PubMed:17609370, PubMed:29899519). May also play a role in regulating glucose homeostasis and insulin sensitivity (PubMed:15837923, PubMed:29899519). Inhibits proliferation, migration, and tubule formation of endothelial cells and reduces vascular leakage (PubMed:14583458, PubMed:17130448, PubMed:21832056). Upon heterologous expression, inhibits the adhesion of endothelial cell to the extracellular matrix (ECM), and inhibits the reorganization of the actin cytoskeleton, formation of actin stress fibers and focal adhesions in endothelial cells that have adhered to ANGPTL4-containing ECM (in vitro) (By similarity). Depending on context, may modulate tumor-related angiogenesis (Probable).
- Post-translational modifications
N-glycosylated.
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Angiopoietin-like 4 also known as ANGPTL4 like4 or ANGPTL4 protein is a glycoprotein with an approximate mass of 50 kDa. It plays a role in lipid metabolism by inhibiting lipoprotein lipase which reduces the breakdown of triglycerides. ANGPTL4 protein is secreted by liver adipose tissue and placenta showing expression in response to fasting and during low insulin states. This expression pattern connects its function to energy homeostasis and metabolic regulation.
- Biological function summary
ANGPTL4 protein influences processes such as lipid metabolism angiogenesis and wound healing. It does not integrate into a large protein complex but functions independently to exert its effects. Inhibition of lipoprotein lipase by ANGPTL4 decreases adipose tissue fat storage and modulates plasma lipid levels. This modulation plays a critical role in maintaining energy balance and glucose homeostasis particularly under metabolic stress conditions.
- Pathways
The functionality of ANGPTL4 protein intersects with the PPAR (Peroxisome Proliferator-Activated Receptor) signaling pathway and the glucose homeostasis pathway. It interacts with other proteins such as PPAR-alpha and PPAR-gamma which regulate genes involved in fatty acid oxidation and lipid transport. This interaction influences the body's response to fasting and caloric restriction by altering lipid metabolism and ensuring efficient energy use.
- Associated diseases and disorders
ANGPTL4 protein correlates with cardiovascular diseases and cancer. Elevated levels of ANGPTL4 associate with an increased risk for atherosclerosis due to its role in lipid metabolism disturbance. In cancer particularly gastric cancer ANGPTL4 influences tumor metastasis by promoting angiogenesis. Its effects on these diseases link to proteins like VEGF in angiogenesis and other metabolic regulators in cardiovascular conditions.
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SDS-PAGE - Recombinant Mouse Angiopoietin-like 4/ANGPTL4 protein (His tag) (ab276823)
SDS-PAGE analysis of ab276823
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