Recombinant Mouse Aqp2 Protein (His Tag) is a Mouse Full Length protein, in the 1 to 271 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE.
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Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- 10x His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M W E L R S I A F S R A V L A E F L A T L L F V F F G L G S A L Q W A S S P P S V L Q I A V A F G L G I G T L V Q A L G H V S G A H I N P A V T V A C L V G C H V S F L R A A F Y V A A Q L L G A V A G A A I L H E I T P V E I R G D L A V N A L H N N A T A G Q A V T V E L F L T M Q L V L C I F A S T D E R R S D N L G S P A L S I G F S V T L G H L L G I Y F T G C S M N P A R S L A P A V V T G K F D D H W V F W I G P L V G A V I G S L L Y N Y L L F P S T K S L Q E R L A V L K G L E P D T D W E E R E V R R R Q S V E L H S P Q S L P R G S K A
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient (PubMed:10191086). Plays an essential role in renal water homeostasis (PubMed:16641094). Could also be permeable to glycerol (By similarity).
Alternative names
cph, Aqp2, Aquaporin-2, AQP-2, ADH water channel, Aquaporin-CD, Collecting duct water channel protein, WCH-CD, Water channel protein for renal collecting duct, AQP-CD
Recommended products
Recombinant Mouse Aqp2 Protein (His Tag) is a Mouse Full Length protein, in the 1 to 271 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- 10x His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P56402-1
- Animal free
- Yes
- Species
- Mouse
- Reconstitution
- We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%.
- Concentration
- Storage buffer
pH: 7.4 - 8
Constituents: 6% Trehalose, 0.87% Sodium chloride, 0.24% Tris, 0.05% 2-Octadecoxyethanol
Sequence info
Amino acid sequence
- M W E L R S I A F S R A V L A E F L A T L L F V F F G L G S A L Q W A S S P P S V L Q I A V A F G L G I G T L V Q A L G H V S G A H I N P A V T V A C L V G C H V S F L R A A F Y V A A Q L L G A V A G A A I L H E I T P V E I R G D L A V N A L H N N A T A G Q A V T V E L F L T M Q L V L C I F A S T D E R R S D N L G S P A L S I G F S V T L G H L L G I Y F T G C S M N P A R S L A P A V V T G K F D D H W V F W I G P L V G A V I G S L L Y N Y L L F P S T K S L Q E R L A V L K G L E P D T D W E E R E V R R R Q S V E L H S P Q S L P R G S K A
- Accession
- P56402
- Protein length
- Full Length
- Actual molecular weight
- 30.5 kDa
- Amino acids
- 1 to 271
- Nature
- Recombinant
- Tags
- 10x His tag N-Terminus
Specifications
- Form
- Lyophilized
General info
- Function
Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient (PubMed:10191086). Plays an essential role in renal water homeostasis (PubMed:16641094). Could also be permeable to glycerol (By similarity).
- Sequence similarities
Belongs to the MIP/aquaporin (TC 1.A.8) family.
- Post-translational modifications
Ser-256 phosphorylation is necessary and sufficient for expression at the apical membrane (PubMed:16641094). Endocytosis is not phosphorylation-dependent (By similarity).
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Add glycerol to a final volume of 50% for extra stability and aliquot
- Storage information
- The product can be stored for up to 12 months
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Aquaporin 2 often referred to as AQP2 or aquaporin-2 channels is a water channel protein facilitating water transport across cell membranes. This protein belongs to the larger family of aquaporins which includes variants such as aquaporine often referred to interchangeably. Aquaporin 2 is a glycosylated integral membrane protein with an estimated mass of about 29 kDa. It expresses mainly in the kidney specifically in the collecting duct cells where it plays a pivotal role in water reabsorption.
- Biological function summary
Aquaporin 2 serves a critical function in maintaining body water balance homeostasis. AQP2 responds to vasopressin a hormone that triggers its translocation to the apical membrane of collecting duct cells enabling water permeability. It is not part of a broader protein complex but operates independently within the cell membrane. This activity regulates urine concentration contributing to the maintenance of body fluids under variable hydration conditions.
- Pathways
Aquaporin 2 contributes prominently to the vasopressin-regulated water reabsorption pathway and relates to the renal water reabsorption pathway. These pathways are important for interpreting how water homeostasis is managed by the body. Proteins such as the vasopressin receptor (also known as AVPR2) interact directly with AQP2 enabling its regulation and function. The interaction with cAMP signaling pathways facilitates the relocation of aquaporin 2 to its active site on the cell membrane ensuring efficient water reabsorption.
- Associated diseases and disorders
Mutations or dysregulation in aquaporin 2 can cause conditions like nephrogenic diabetes insipidus characterized by an inability to concentrate urine leading to excessive urination and thirst. Another disorder associated with AQP2 is syndrome of inappropriate antidiuretic hormone secretion (SIADH). These conditions highlight the importance of aquaporin 2 in fluid homeostasis and they connect it to regulatory proteins such as vasopressin itself emphasizing the interconnected nature of cellular proteins in disease mechanisms.
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1 product image
SDS-PAGE - Recombinant Mouse Aqp2 Protein (His Tag) (ab314609)
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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