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AB276746

Recombinant Mouse ARSA/ASA protein (His tag)

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Recombinant Mouse ARSA/ASA protein (His tag) is a Mouse Full Length protein, in the 1 to 506 aa range, expressed in HEK 293 cells, with >97%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE.

View Alternative Names

As2, Arsa, Arylsulfatase A, ASA, Cerebroside-sulfatase

1 Images
SDS-PAGE - Recombinant Mouse ARSA/ASA protein (His tag) (AB276746)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Mouse ARSA/ASA protein (His tag) (AB276746)

SDS-PAGE analysis of ab276746

Key facts

Purity

>97% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

HEK 293 cells

Tags

His tag C-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

P50428

Animal free

No

Carrier free

No

Species

Mouse

Storage buffer

pH: 7.4 Constituents: 0.3% Tris, 0.00087% Sodium chloride

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MALGTLFLALAAGLSTASPPNILLIFADDLGYGDLGSYGHPSSTTPNLDQLAEGGLRFTDFYVPVSLCTPSRAALLTGRLPVRSGMYPGVLGPSSQGGLPLEEVTLAEVLAARGYLTGMAGKWHLGVGPEGAFLPPHQGFHRFLGIPYSHDQGPCQNLTCFPPDIPCKGGCDQGLVPIPLLANLTVEAQPPWLPGLEARYVSFSRDLMADAQRQGRPFFLYYASHHTHYPQFSGQSFTKRSGRGPFGDSLMELDGAVGALMTTVGDLGLLEETLVIFTADNGPELMRMSNGGCSGLLRCGKGTTFEGGVREPALVYWPGHITPGVTHELASSLDLLPTLAALTGAPLPNVTLDGVDISPLLLGTGKSPRKSVFFYPPYPDEIHGVFAVRNGKYKAHFFTQGSAHSDTTSDPACHAANRLTAHEPPLLYDLSQDPGENYNVLESIEGVSPEALQALKHIQLLKAQYDAAMTFGPSQIAKGEDPALQICCQPSCTPHPVCCHCPGSQS","proteinLength":"Full Length","predictedMolecularWeight":"53.5 kDa","actualMolecularWeight":null,"aminoAcidEnd":506,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P50428","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ARSA protein also known as arylsulfatase A or ASA protein functions mechanically as a lysosomal enzyme responsible for the cleavage of cerebroside sulfate into cerebroside and sulfate. It belongs to a family of enzymes important for the hydrolysis of sulfate esters and has a molecular mass of about 62 kDa. This enzyme is expressed mainly in the liver and kidney but also in the brain where it plays important roles in normal functioning.
Biological function summary

ARSA is essential in the breakdown of sulfatides which are components of myelin sheaths in the central nervous system. It functions as part of a lysosomal complex involving cooperators like saposin B which facilitates its interaction with sulfatide substrates. By breaking down sulfatides ARSA helps in cellular membrane maintenance and recycling contributing to neurological function and stability.

Pathways

ARSA is mainly involved in the sphingolipid metabolism pathway. This pathway manages the degradation of sphingolipids important for maintaining neuron and cell membrane integrity. ARSA also interacts with proteins such as galactosylceramidase as both play roles in lipid recycling and are essential for maintaining cell health within the nervous system.

ARSA's deficiency is a well-known factor in metachromatic leukodystrophy (MLD) a genetic disorder leading to progressive leukodystrophy. This condition results from sulfatide accumulation in the nervous system caused by insufficient ARSA activity. The disease involves other proteins such as saposin B which when dysfunctional exacerbate ARSA-related pathologies and symptoms. Another disorder related to defective ARSA function includes chronic kidney disease where compromised sulfatide metabolism further effects cellular processes.
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Specifications

Form

Lyophilized

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General info

Function

Hydrolyzes cerebroside sulfate.

Sequence similarities

Belongs to the sulfatase family.

Post-translational modifications

The conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity. This post-translational modification is severely defective in multiple sulfatase deficiency (MSD).

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Product protocols

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Target data

Hydrolyzes cerebroside sulfate.
See full target information Arsa
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