Recombinant Mouse ARSA/ASA protein (His tag) is a Mouse Full Length protein, in the 1 to 506 aa range, expressed in HEK 293, with >97% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
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Key facts
- Purity
>97% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
HEK 293 cells
- Tags
His tag C-Terminus
- Applications
SDS-PAGE
- Biologically active
No
Amino acid sequence
M A L G T L F L A L A A G L S T A S P P N I L L I F A D D L G Y G D L G S Y G H P S S T T P N L D Q L A E G G L R F T D F Y V P V S L C T P S R A A L L T G R L P V R S G M Y P G V L G P S S Q G G L P L E E V T L A E V L A A R G Y L T G M A G K W H L G V G P E G A F L P P H Q G F H R F L G I P Y S H D Q G P C Q N L T C F P P D I P C K G G C D Q G L V P I P L L A N L T V E A Q P P W L P G L E A R Y V S F S R D L M A D A Q R Q G R P F F L Y Y A S H H T H Y P Q F S G Q S F T K R S G R G P F G D S L M E L D G A V G A L M T T V G D L G L L E E T L V I F T A D N G P E L M R M S N G G C S G L L R C G K G T T F E G G V R E P A L V Y W P G H I T P G V T H E L A S S L D L L P T L A A L T G A P L P N V T L D G V D I S P L L L G T G K S P R K S V F F Y P P Y P D E I H G V F A V R N G K Y K A H F F T Q G S A H S D T T S D P A C H A A N R L T A H E P P L L Y D L S Q D P G E N Y N V L E S I E G V S P E A L Q A L K H I Q L L K A Q Y D A A M T F G P S Q I A K G E D P A L Q I C C Q P S C T P H P V C C H C P G S Q S
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Alternative names
As2, Arsa, Arylsulfatase A, ASA, Cerebroside-sulfatase
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Recombinant Mouse ARSA/ASA protein (His tag) is a Mouse Full Length protein, in the 1 to 506 aa range, expressed in HEK 293, with >97% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Key facts
- Purity
>97% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
HEK 293 cells
- Tags
His tag C-Terminus
- Applications
SDS-PAGE
- Biologically active
No
- Accession
- P50428-1
- Animal free
No
- Species
Mouse
- Concentration
- Storage buffer
pH: 7.4
Constituents: 0.3% Tris, 0.00087% Sodium chloride
Sequence info
Amino acid sequence
M A L G T L F L A L A A G L S T A S P P N I L L I F A D D L G Y G D L G S Y G H P S S T T P N L D Q L A E G G L R F T D F Y V P V S L C T P S R A A L L T G R L P V R S G M Y P G V L G P S S Q G G L P L E E V T L A E V L A A R G Y L T G M A G K W H L G V G P E G A F L P P H Q G F H R F L G I P Y S H D Q G P C Q N L T C F P P D I P C K G G C D Q G L V P I P L L A N L T V E A Q P P W L P G L E A R Y V S F S R D L M A D A Q R Q G R P F F L Y Y A S H H T H Y P Q F S G Q S F T K R S G R G P F G D S L M E L D G A V G A L M T T V G D L G L L E E T L V I F T A D N G P E L M R M S N G G C S G L L R C G K G T T F E G G V R E P A L V Y W P G H I T P G V T H E L A S S L D L L P T L A A L T G A P L P N V T L D G V D I S P L L L G T G K S P R K S V F F Y P P Y P D E I H G V F A V R N G K Y K A H F F T Q G S A H S D T T S D P A C H A A N R L T A H E P P L L Y D L S Q D P G E N Y N V L E S I E G V S P E A L Q A L K H I Q L L K A Q Y D A A M T F G P S Q I A K G E D P A L Q I C C Q P S C T P H P V C C H C P G S Q S
- Accession
- P50428
- Protein length
Full Length
- Predicted molecular weight
53.5 kDa
- Amino acids
1 to 506
- Nature
Recombinant
- Tags
His tag C-Terminus
Specifications
- Form
Lyophilized
General info
- Function
Hydrolyzes cerebroside sulfate.
- Sequence similarities
Belongs to the sulfatase family.
- Post-translational modifications
The conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity. This post-translational modification is severely defective in multiple sulfatase deficiency (MSD).
- Subcellular localisation
Lysosome
Storage
- Shipped at conditions
Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The ARSA protein also known as arylsulfatase A or ASA protein functions mechanically as a lysosomal enzyme responsible for the cleavage of cerebroside sulfate into cerebroside and sulfate. It belongs to a family of enzymes important for the hydrolysis of sulfate esters and has a molecular mass of about 62 kDa. This enzyme is expressed mainly in the liver and kidney but also in the brain where it plays important roles in normal functioning.
- Biological function summary
ARSA is essential in the breakdown of sulfatides which are components of myelin sheaths in the central nervous system. It functions as part of a lysosomal complex involving cooperators like saposin B which facilitates its interaction with sulfatide substrates. By breaking down sulfatides ARSA helps in cellular membrane maintenance and recycling contributing to neurological function and stability.
- Pathways
ARSA is mainly involved in the sphingolipid metabolism pathway. This pathway manages the degradation of sphingolipids important for maintaining neuron and cell membrane integrity. ARSA also interacts with proteins such as galactosylceramidase as both play roles in lipid recycling and are essential for maintaining cell health within the nervous system.
- Associated diseases and disorders
ARSA's deficiency is a well-known factor in metachromatic leukodystrophy (MLD) a genetic disorder leading to progressive leukodystrophy. This condition results from sulfatide accumulation in the nervous system caused by insufficient ARSA activity. The disease involves other proteins such as saposin B which when dysfunctional exacerbate ARSA-related pathologies and symptoms. Another disorder related to defective ARSA function includes chronic kidney disease where compromised sulfatide metabolism further effects cellular processes.
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SDS-PAGE - Recombinant Mouse ARSA/ASA protein (His tag) (ab276746)
SDS-PAGE analysis of ab276746
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