Recombinant Mouse B2M + FCGRT protein (Tagged) (Biotin) is a Mouse Full Length protein, in the 1 to 119 aa range, expressed in HEK 293, with >=90% purity and suitable for SDS-PAGE.
Images
Key facts
- Purity
- >=90% SDS-PAGE
- Expression system
- HEK 293 cells
- Tags
- Avi tag C-Terminus His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Conjugation
- Biotin
Sequence
This product is comprised of multiple sequences see
Amino acid sequence 1
M A R S V T L V F L V L V S L T G L Y A I Q K T P Q I Q V Y S R H P P E N G K P N I L N C Y V T Q F H P P H I E I Q M L K N G K K I P K V E M S D M S F S K D W S F Y I L A H T E F T P T E T D T Y A C R V K H A S M A E P K T V Y W D R D M
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Cell surface receptor that transfers passive humoral immunity from the mother to the newborn. Binds to the Fc region of monomeric immunoglobulin gamma and mediates its selective uptake from milk (PubMed:7504013). IgG in the milk is bound at the apical surface of the intestinal epithelium. The resultant FcRn-IgG complexes are transcytosed across the intestinal epithelium and IgG is released from FcRn into blood or tissue fluids. Throughout life, contributes to effective humoral immunity by recycling IgG and extending its half-life in the circulation. Mechanistically, monomeric IgG binding to FcRn in acidic endosomes of endothelial and hematopoietic cells recycles IgG to the cell surface where it is released into the circulation. In addition of IgG, regulates homeostasis of the other most abundant circulating protein albumin/ALB.
Alternative names
Fcrn, Fcgrt, IgG receptor FcRn large subunit p51, FcRn, IgG Fc fragment receptor transporter alpha chain, Neonatal Fc receptor
Recommended products
Recombinant Mouse B2M + FCGRT protein (Tagged) (Biotin) is a Mouse Full Length protein, in the 1 to 119 aa range, expressed in HEK 293, with >=90% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >=90% SDS-PAGE
- Expression system
- HEK 293 cells
- Tags
- Avi tag C-Terminus His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Conjugation
- Biotin
- Accession
- Q61559-1
- Animal free
- No
- Species
- Mouse
- Concentration
- Storage buffer
pH: 7.4
Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.13% Sodium phosphate, 0.02% Potassium chloride
Sequence info
Amino acid sequence 1
- M A R S V T L V F L V L V S L T G L Y A I Q K T P Q I Q V Y S R H P P E N G K P N I L N C Y V T Q F H P P H I E I Q M L K N G K K I P K V E M S D M S F S K D W S F Y I L A H T E F T P T E T D T Y A C R V K H A S M A E P K T V Y W D R D M
- Accession
- P01887
- Protein length
- Full Length
- Amino acids
- 1 to 119
- Nature
- Recombinant
Amino acid sequence 2
- S E T R P P L M Y H L T A V S N P S T G L P S F W A T G W L G P Q Q Y L T Y N S L R Q E A D P C G A W M W E N Q V S W Y W E K E T T D L K S K E Q L F L E A L K T L E K I L N G T Y T L Q G L L G C E L A S D N S S V P T A V F A L N G E E F M K F N P R I G N W T G E W P E T E I V A N L W M K Q P D A A R K E S E F L L N S C P E R L L G H L E R G R R N L E W K E P P S M R L K A R P G N S G S S V L T C A A F S F Y P P E L K F R F L R N G L A S G S G N C S T G P N G D G S F H A W S L L E V K R G D E H H Y Q C Q V E H E G L A Q P L T V D L D S S A R S S
- Accession
- Q61559
- Protein length
- Fragment
- Amino acids
- 22 to 297
- Nature
- Recombinant
- Tags
- Avi tag C-Terminus, His tag C-Terminus
Specifications
- Form
- Liquid
General info
- Function
Cell surface receptor that transfers passive humoral immunity from the mother to the newborn. Binds to the Fc region of monomeric immunoglobulin gamma and mediates its selective uptake from milk (PubMed:7504013). IgG in the milk is bound at the apical surface of the intestinal epithelium. The resultant FcRn-IgG complexes are transcytosed across the intestinal epithelium and IgG is released from FcRn into blood or tissue fluids. Throughout life, contributes to effective humoral immunity by recycling IgG and extending its half-life in the circulation. Mechanistically, monomeric IgG binding to FcRn in acidic endosomes of endothelial and hematopoietic cells recycles IgG to the cell surface where it is released into the circulation. In addition of IgG, regulates homeostasis of the other most abundant circulating protein albumin/ALB.
- Sequence similarities
Belongs to the immunoglobulin superfamily.
- Subcellular localisation
- Endosome membrane
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Storage information
- Avoid freeze / thaw cycle, Store in the dark
Notes
Enzymatically biotin-labeled using Avi-tag™ technology
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
B2M (Beta-2-Microglobulin) and FCGRT (Neonatal Fc Receptor) form a cellular complex. B2M is a small protein with a mass of around 11.8 kDa while FCGRT is a receptor that binds to the Fc region of IgG immunoglobulins. This complex plays an important role in the recycling and transport of IgG antibodies. B2M & FCGRT are expressed in various tissues including placenta kidney and epithelial cells. Together they help in the protection and long half-life of IgG by preventing its degradation in lysosomes.
- Biological function summary
The B2M and FCGRT complex ensures proper IgG homeostasis in the body. This process allows the maintenance of high levels of circulating IgG without constant new synthesis. The B2M & FCGRT protein complex engages in transcytosis and recycling which are important for immune surveillance and pathogen neutralization. B2M associates with the major histocompatibility complex (MHC) class I molecules further aiding in antigen presentation alongside its role in the B2M protein complex.
- Pathways
B2M & FCGRT interaction is part of the IgG salvage pathway. This pathway efficiently recycles IgG antibodies a step necessary for sustained immunity. The FCGRT receptor relies on the association with proteins like albumin to maintain this recycling route. B2M is also known to affect pathways including antigen processing and cross-presentation connecting it with proteins involved in adaptive immune responses.
- Associated diseases and disorders
B2M & FCGRT have implications in autoimmune diseases and nephropathies. Alterations in the function or expression of this complex can affect IgG recycling and antibody dynamics influencing autoimmune conditions like systemic lupus erythematosus. Furthermore B2M levels can serve as a marker in renal disorders particularly in glomerulonephritis. Dysregulation often aligns with proteins and receptors involved in immune regulation highlighting their role in disease progression.
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1 product image
SDS-PAGE - Recombinant Mouse B2M + FCGRT protein (Tagged) (Biotin) (ab271510)
SDS-PAGE analysis of 5 μg ab271510.
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