Recombinant mouse BAFF protein
- Bioactive
- What is this?
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(1 Publication)
Recombinant mouse BAFF protein is a Mouse Full Length protein, in the 127 to 309 aa range, expressed in HEK 293 cells, with >95%, < 0.1 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS.
View Alternative Names
CD257, Baff, Tnfsf13b, Tumor necrosis factor ligand superfamily member 13B, B-cell-activating factor, BAFF
- FuncS
Unknown
Functional Studies - Recombinant mouse BAFF protein (AB157285)
ab157285 mediates survival of freshly isolated splenocytes. Method : On day 0 splenocytes were isolated from a freshly collected C57Bl6 spleen. An aliquot of the splenocytes was analyzed on FACS and gated on the SSC-FSC panel. FACS settings were saved. The rest of the cells was put in culture with media alone or with increasing concentrations of ab157285 as indicated. After three days in culture, cells were harvested and analyzed on FACS with the saved setting. Splenocytes Survival Index (ratio % living/% dead cells) was calculated and plotted.
- SDS-PAGE
Unknown
SDS-PAGE - Recombinant mouse BAFF protein (AB157285)
SDS-PAGE analysis : Lane 1 : MWt Marker, Lane 2 : 1 μg ab157285, stained with Imperial stain
Reactivity data
Product details
Mediates splenocyte survival.
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
BAFF plays a significant role in the humoral immune response by enhancing B-cell proliferation and function. It belongs to the tumor necrosis factor (TNF) ligand family and interacts as a homotrimer. The BAFF cytokine can bind to receptors such as BAFF-R BCMA (B-cell maturation antigen) and TACI (transmembrane activator and CAML interactor). These interactions regulate B-cell homeostasis promoting development and differentiation processes important for normal immune responses.
Pathways
BAFF is central to the regulation of immune system signaling particularly in B-cell receptor (BCR) signaling and NF-kB pathway activation. The BAFF-R mediates B-cell survival signals through the NF-kB pathway while TACI and BCMA associations convey additional immune responses regulation. These multiple pathways ensure the maintenance of B-cell populations and their ability to produce antibodies.
Specifications
Form
Lyophilized
General info
Function
Cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B- and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response.. Isoform 2 seems to inhibit isoform 1 secretion and bioactivity.
Sequence similarities
Belongs to the tumor necrosis factor family.
Post-translational modifications
The soluble form derives from the membrane form by proteolytic processing.. Isoform 2 is not efficiently shed from the membrane unlike isoform 1.
Target data
Publications (1)
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JCI insight 5: PubMed32434989
2020
Applications
Unspecified application
Species
Unspecified reactive species
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