Recombinant Mouse BAFF protein is a Mouse Fragment protein, in the 127 to 309 aa range, expressed in HEK 293, with >=95% purity and suitable for SDS-PAGE.
Images
Key facts
- Purity
- >=95% SDS-PAGE
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
A F Q G P E E T E Q D V D L S A P P A P C L P G C R H S Q H D D N G M N L R N I I Q D C L Q L I A D S D T P T I R K G T Y T F V P W L L S F K R G N A L E E K E N K I V V R Q T G Y F F I Y S Q V L Y T D P I F A M G H V I Q R K K V H V F G D E L S L V T L F R C I Q N M P K T L P N N S C Y S A G I A R L E E G D E I Q L A I P R E N A Q I S R N G D D T F F G A L K L L
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B- and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response. Isoform 2 seems to inhibit isoform 1 secretion and bioactivity.
Alternative names
CD257, Baff, Tnfsf13b, Tumor necrosis factor ligand superfamily member 13B, B-cell-activating factor, BAFF
Recommended products
Recombinant Mouse BAFF protein is a Mouse Fragment protein, in the 127 to 309 aa range, expressed in HEK 293, with >=95% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >=95% SDS-PAGE
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- Q9WU72-1
- Animal free
- Yes
- Carrier free
- Yes
- Species
- Mouse
- Reconstitution
- Reconstitute in PBS
- Concentration
- Storage buffer
pH: 7.4
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic
Sequence info
Amino acid sequence
- A F Q G P E E T E Q D V D L S A P P A P C L P G C R H S Q H D D N G M N L R N I I Q D C L Q L I A D S D T P T I R K G T Y T F V P W L L S F K R G N A L E E K E N K I V V R Q T G Y F F I Y S Q V L Y T D P I F A M G H V I Q R K K V H V F G D E L S L V T L F R C I Q N M P K T L P N N S C Y S A G I A R L E E G D E I Q L A I P R E N A Q I S R N G D D T F F G A L K L L
- Accession
- Q9WU72
- Protein length
- Fragment
- Predicted molecular weight
- 20.68 kDa
- Amino acids
- 127 to 309
- Nature
- Recombinant
Specifications
- Form
- Lyophilized
General info
- Function
Cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B- and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response.
- Sequence similarities
Belongs to the tumor necrosis factor family.
- Post-translational modifications
The soluble form derives from the membrane form by proteolytic processing.
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- Ambient
- Appropriate long-term storage conditions
- Ambient
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
BAFF also known as B-cell activating factor or TNFSF13B is a protein important for B-cell survival and maturation. It's a type II transmembrane protein expressed in cells like macrophages and dendritic cells and has a mass of approximately 31 kDa. The BAFF protein is also present on activated T-cells and in the serum as a soluble form. It functions as a cytokine and influences various stages of B-cell development.
- Biological function summary
BAFF plays a significant role in the humoral immune response by enhancing B-cell proliferation and function. It belongs to the tumor necrosis factor (TNF) ligand family and interacts as a homotrimer. The BAFF cytokine can bind to receptors such as BAFF-R BCMA (B-cell maturation antigen) and TACI (transmembrane activator and CAML interactor). These interactions regulate B-cell homeostasis promoting development and differentiation processes important for normal immune responses.
- Pathways
BAFF is central to the regulation of immune system signaling particularly in B-cell receptor (BCR) signaling and NF-kB pathway activation. The BAFF-R mediates B-cell survival signals through the NF-kB pathway while TACI and BCMA associations convey additional immune responses regulation. These multiple pathways ensure the maintenance of B-cell populations and their ability to produce antibodies.
- Associated diseases and disorders
BAFF has been linked to conditions such as systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA). Elevated levels of BAFF can exacerbate autoimmune responses due to increased B-cell survival. Connections with these disorders involve the BAFF-R and its influence on excessive B-cell proliferation contributing to disease pathology. Therapeutically anti-BAFF therapies target these interactions to modify disease progression and manage autoimmunity.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
SDS-PAGE - Recombinant Mouse BAFF protein (ab283484)
SDS-PAGE analysis of ab283484.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com