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Recombinant Mouse Betacellulin Protein Standard (His tag) is a Mouse Fragment protein, expressed in HEK 293, with >=80% purity and suitable for SDS-PAGE, sELISA.

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Images

Sandwich ELISA - Recombinant Mouse Betacellulin Protein Standard (His tag) (AB316731), expandable thumbnail
  • SDS-PAGE - Recombinant Mouse Betacellulin Protein Standard (His tag) (AB316731), expandable thumbnail

Key facts

Purity

>=80% SDS-PAGE

Expression system

HEK 293 cells

Tags

His tag C-Terminus

Applications

SDS-PAGE, sELISA

Biologically active

No

Reactivity data

Application

SDS-PAGE

Reactivity

Reacts

Dilution info

-

Notes

-

Application

sELISA

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Growth factor that binds to EGFR, ERBB4 and other EGF receptor family members. Potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells.

Alternative names

Recommended products

Recombinant Mouse Betacellulin Protein Standard (His tag) is a Mouse Fragment protein, expressed in HEK 293, with >=80% purity and suitable for SDS-PAGE, sELISA.

Key facts

Purity

>=80% SDS-PAGE

Expression system

HEK 293 cells

Applications

SDS-PAGE, sELISA

Accession
Q05928-1
Animal free

Yes

Species

Mouse

Concentration
Loading...
Storage buffer

pH: 7.3 - 7.5
Constituents: 2.922% Sodium chloride, 0.64107% disodium;hydrogen phosphate;dodecahydrate, 0.02858% Potassium phosphate monobasic

Sequence info

Amino acid sequence

Accession

Q05928

Protein length

Fragment

Predicted molecular weight

29.9 kDa

Nature

Recombinant

Tags

His tag C-Terminus

Specifications

Form

Liquid

General info

Function

Growth factor that binds to EGFR, ERBB4 and other EGF receptor family members. Potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells.

Storage

Shipped at conditions

Dry Ice

Appropriate short-term storage conditions

-80°C

Appropriate long-term storage conditions

-80°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

While the standard is the same as the one provided in the corresponding SimpleStep ELISA Kit, it cannot be treated as the consumable provided with our SimpleStep ELISA Kit due to differences in its concentration calibration.

Abcam guarantee that this protein standard is suitable for use in a sandwich ELISA. Individual results may vary due to differences in technique, laboratory equipment, buffers, and other experimental factors. The detection range provided for this protein standard is based on initial sandwich ELISA validation data.

The protein concentration is the concentration after validation on our sandwich ELISA platform. This Standard protein is guaranteed to work with our Capture and Detector antibodies in sELISA. Please contact our Scientific Support team to know which antibody pair is suitable for this protein.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The BTC protein also known as betacellulin is a member of the epidermal growth factor (EGF) family that plays an important role in cell signaling. It is a growth factor with a molecular weight of approximately 32 kDa. BTC is expressed in a range of tissues including the pancreas kidney and small intestine. This protein binds to and activates the EGF receptor (EGFR) stimulating various cellular processes. Researchers often use the BTC mouse and BTC chimeras to study its function and physiological roles due to their relevance to human biology.

Biological function summary

BTC plays an important role in regulating cell proliferation especially in the pancreas where it influences beta-cell growth and regeneration. It operates as part of a signaling complex that interacts with other members of the EGF family impacting various cellular responses. Researchers employ experimental tools like BTC ELISA kits to quantify BTC levels in biological samples aiding in the exploration of its biological functions and mechanisms. BTC’s ability to modulate growth pathways makes it a significant molecule under investigation.

Pathways

BTC participates in the EGF/EGFR signaling pathway which is essential for controlling cellular growth and differentiation. This pathway involves the interaction of BTC with other ligands such as EGF and transforming growth factor-alpha (TGF-α) altering downstream signaling events. BTC further contributes to the activation of the MAPK/ERK pathway a critical process for transmitting signals from the cell surface to the DNA in the nucleus. Through these pathways BTC impacts cellular activities and balances multiple biological functions.

Associated diseases and disorders

BTC is linked to conditions such as diabetes and various cancers. In diabetes BTC is associated with pancreatic function particularly influencing insulin production through its role in beta-cell regulation. In cancer abnormal BTC expression or its interactions with EGFR signaling components can lead to uncontrolled cell proliferation. Researchers study BTC’s interactions with proteins like EGF and TGF-α to understand its involvement in these disorders as therapeutic targeting of this axis holds potential for treatment strategies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Sandwich ELISA - Recombinant Mouse Betacellulin Protein Standard (His tag) (ab316731), expandable thumbnail

    Sandwich ELISA - Recombinant Mouse Betacellulin Protein Standard (His tag) (ab316731)

    Sandwich ELISA with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.

  • SDS-PAGE - Recombinant Mouse Betacellulin Protein Standard (His tag) (ab316731), expandable thumbnail

    SDS-PAGE - Recombinant Mouse Betacellulin Protein Standard (His tag) (ab316731)

    SDS-PAGE analysis of ab316731 under reducing conditions for 2ug protein.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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