Recombinant Mouse C5 protein is a Mouse Fragment protein, in the 679 to 755 aa range, expressed in HEK 293 cells, with >95%, <0.005 EU/µg endotoxin level, suitable for SDS-PAGE, Mass Spec, HPLC.
View Alternative Names
Hc, C5, Complement C5, Hemolytic complement
- Mass Spec
Supplier Data
Mass Spectrometry - Recombinant Mouse C5 protein (AB283428)
Mass determination by ESI-TOF.
Predicted MW is 8958.44 Da (+/- 10 Da by ESI-TOF). Observed MW is 8802.38 Da. -156.2 Da adjustment for the Arginine truncation.
- HPLC
Supplier Data
HPLC - Recombinant Mouse C5 protein (AB283428)
HPLC analysis of ab283428.
- SDS-PAGE
Supplier Data
SDS-PAGE - Recombinant Mouse C5 protein (AB283428)
SDS-PAGE analysis of ab283428.
Reactivity data
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The role of C5 and its cleavage products are significant in the immune response. C5a a powerful chemoattractant promotes recruitment of neutrophils and other inflammatory cells at sites of infection. C5b initiates the formation of the membrane attack complex (MAC) which is important for lysing pathogens. C5a as part of the C5/C5a axis plays a vital role in regulating immune cell activity and modulating inflammatory processes. The protein is part of the terminal complement complex engaging in immune defense mechanisms.
Pathways
C5 is integral to the classical and alternative complement pathways. In both pathways the cleavage of C5 is a critical step that leads to the eventual formation of the MAC. C5 interacts with several proteins including C3 and C6 to execute its functions effectively. The close relationship between C3 activation and C5 conversion highlights the interconnectedness of complement proteins in these pathways ensuring a robust immune response against pathogens.
Specifications
Form
Lyophilized
Additional notes
=95% Purity determined by HPLC and SDS-PAGE
General info
Function
Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled.. C5a anaphylatoxin. Derived from proteolytic degradation of complement C5, C5a anaphylatoxin is a mediator of local inflammatory process. Binding to the receptor C5AR1 induces a variety of responses including intracellular calcium release, contraction of smooth muscle, increased vascular permeability, and histamine release from mast cells and basophilic leukocytes. C5a is also a potent chemokine which stimulates the locomotion of polymorphonuclear leukocytes and directs their migration toward sites of inflammation.
Target data
Complementary Products
Primary Antibodies
AB275931
Anti-C5 / C5b antibody [EPR23940-3]
BOND RX™ Validated|KO Validated|RabMAb|Recombinant
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C5 / C5b antibody [EPR23940-3] (AB275931)](https://content.abcam.com/products/images/c5-c5b-antibody-epr23940-3-ab275931--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img112895.jpg)
- 0
- 0
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com