Recombinant Mouse Cardiac Troponin I protein (His tag) is a Mouse Full Length protein, in the 2 to 211 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
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Key facts
- Purity
>90% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE
- Biologically active
No
Amino acid sequence
A D E S S D A A G E P Q P A P A P V R R R S S A N Y R A Y A T E P H A K K K S K I S A S R K L Q L K T L M L Q I A K Q E M E R E A E E R R G E K G R V L R T R C Q P L E L D G L G F E E L Q D L C R Q L H A R V D K V D E E R Y D V E A K V T K N I T E I A D L T Q K I Y D L R G K F K R P T L R R V R I S A D A M M Q A L L G T R A K E S L D L R A H L K Q V K K E D I E K E N R E V G D W R K N I D A L S G M E G R K K K F E G
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
Alternative names
Cardiac troponin I, Tnni3
Recommended products
Recombinant Mouse Cardiac Troponin I protein (His tag) is a Mouse Full Length protein, in the 2 to 211 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
Key facts
- Purity
>90% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE
- Biologically active
No
- Accession
- P48787-1
- Animal free
No
- Species
Mouse
- Concentration
- Storage buffer
pH: 7.2 - 7.4
Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
A D E S S D A A G E P Q P A P A P V R R R S S A N Y R A Y A T E P H A K K K S K I S A S R K L Q L K T L M L Q I A K Q E M E R E A E E R R G E K G R V L R T R C Q P L E L D G L G F E E L Q D L C R Q L H A R V D K V D E E R Y D V E A K V T K N I T E I A D L T Q K I Y D L R G K F K R P T L R R V R I S A D A M M Q A L L G T R A K E S L D L R A H L K Q V K K E D I E K E N R E V G D W R K N I D A L S G M E G R K K K F E G
- Accession
- Protein length
Full Length
- Predicted molecular weight
40.1 kDa
- Amino acids
2 to 211
- Nature
Recombinant
- Tags
His tag N-Terminus
Specifications
- Form
Liquid
General info
- Function
Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
- Sequence similarities
Belongs to the troponin I family.
- Post-translational modifications
Phosphorylated at Ser-23 and Ser-24 by PRKD1; phosphorylation reduces myofilament calcium sensitivity. Phosphorylated preferentially at Thr-32. Phosphorylation by STK4/MST1 alters its binding affinity to TNNC1 (cardiac Tn-C) and TNNT2 (cardiac Tn-T) (By similarity). Phosphorylated at Ser-43 and Ser-45 by PRKCE; phosphorylation increases myocardium contractile dysfunction.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Product promise
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1 product image
SDS-PAGE - Recombinant Mouse Cardiac Troponin I protein (His tag) (ab226425)
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) analysis of ab226425 with 5% enrichment gel and 15% separation gel.
Downloads
Product protocols
- Visit the general protocols
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