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AB283889

Recombinant Mouse CCL11/Eotaxin Protein (Active)

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Recombinant Mouse CCL11/Eotaxin Protein (Active) is a Mouse Fragment protein, in the 26 to 97 aa range, expressed in HEK 293 cells, with >95%, < 0.005 EU/µg endotoxin level, suitable for ELISA, FuncS, SDS-PAGE, Mass Spec, HPLC.

View Alternative Names

Scya11, Ccl11, Eotaxin, C-C motif chemokine 11, Eosinophil chemotactic protein, Small-inducible cytokine A11

3 Images
Mass Spectrometry - Recombinant Mouse CCL11/Eotaxin Protein (Active) (AB283889)
  • Mass Spec

Supplier Data

Mass Spectrometry - Recombinant Mouse CCL11/Eotaxin Protein (Active) (AB283889)

LC-MS analysis of ab283889.

Predicted MW is 8204.81 Da (+/- 10 Da by LCMS). Observed MW is 8204.48 Da.

HPLC - Recombinant Mouse CCL11/Eotaxin Protein (Active) (AB283889)
  • HPLC

Supplier Data

HPLC - Recombinant Mouse CCL11/Eotaxin Protein (Active) (AB283889)

HPLC analysis of ab283889.

SDS-PAGE - Recombinant Mouse CCL11/Eotaxin Protein (Active) (AB283889)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Mouse CCL11/Eotaxin Protein (Active) (AB283889)

SDS-PAGE analysis of ab283889.

Key facts

Purity

>95% SDS-PAGE

Greater than 95% purity by HPLC.

Endotoxin level

< 0.005 EU/µg

Expression system

HEK 293 cells

Tags

Tag free

Applications

Mass Spec, SDS-PAGE, HPLC, ELISA, FuncS

applications

Biologically active

Yes

Biological activity

Protein interaction was confirmed with SimpleStep ELISA kit ab201277. The calibration factor used with the kit was 0.52

Accession

P48298

Animal free

Yes

Carrier free

Yes

Species

Mouse

Reconstitution

Reconstitute in PBS

Storage buffer

pH: 7.4 Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

storage-buffer

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Primary Antibodies

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Secondary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"<p></p>" }, "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"linker":null,"sequence":"GSIPTSCCFIMTSKKIPNTLLKSYKRITNNRCTLKAIVFKTRLGKEICADPKKKWVQDATKHLDQKLQTPKP","proteinLength":"Fragment","predictedMolecularWeight":"8.2 kDa","actualMolecularWeight":"8.2 kDa","aminoAcidEnd":97,"aminoAcidStart":26,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P48298","tags":[]}]
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Properties and storage information

Form
Lyophilized
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
Ambient
Appropriate long-term storage conditions
Ambient
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Eotaxin also known as CCL11 or eotaxin-1 is a chemokine belonging to the CC chemokine family. This protein has a molecular mass of approximately 8.4 kDa. Eotaxin is mainly expressed in epithelial cells fibroblasts and smooth muscle cells. High levels of expression occur during inflammatory processes where it acts as a chemoattractant guiding eosinophils to sites of inflammation.
Biological function summary

Eotaxin plays an important role in immune response regulation particularly in allergic reactions. It functions by binding to CCR3 a receptor found on eosinophils therefore facilitating their migration. This process includes involvement in the formation of protein complexes that modulate cell signaling. Eotaxin's primary biological role lies in promoting the accumulation of eosinophils which are important for the immune defense against parasitic infections.

Pathways

Eotaxin participates in the MAPK/ERK signaling pathway which influences cell migration and proliferation. Additionally it operates within the NF-kB pathway often regulating inflammation and immune response. In these pathways eotaxin interacts with other proteins like CCR3 and IL-5 contributing to a coordinated response in immune signaling.

Eotaxin is closely linked to allergic asthma and atopic dermatitis. In both these conditions eotaxin-mediated eosinophil recruitment and activation worsen inflammation. Research shows significant eotaxin expression in asthmatic airway tissues. It also connects with proteins like IL-5 which amplify eosinophilic activity leading to exacerbated allergic responses.
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General info

Function

Chemokine that plays a central role in both allergic and non-allergic inflammatory reactions by inducing the migration of different leukocyte types including eosinophils, basophils, macrophages and dendritic cells (PubMed : 7568052, PubMed : 8574847). Exerts its effects primarily by binding to the CCR3 receptor. Induces also chemotaxis of endothelial cells and promotes angiogenesis by activating the PI3K/Akt pathway.

Sequence similarities

Belongs to the intercrine beta (chemokine CC) family.

Post-translational modifications

O-linked glycan consists of a Gal-GalNAc disaccharide which is modified with up to 2 sialic acid residues.

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Product protocols

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Target data

Chemokine that plays a central role in both allergic and non-allergic inflammatory reactions by inducing the migration of different leukocyte types including eosinophils, basophils, macrophages and dendritic cells (PubMed : 7568052, PubMed : 8574847). Exerts its effects primarily by binding to the CCR3 receptor. Induces also chemotaxis of endothelial cells and promotes angiogenesis by activating the PI3K/Akt pathway.
See full target information Ccl11
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Product promise

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