Recombinant mouse CD105 protein (His tag) is a Mouse Fragment protein, in the 1 to 580 aa range, expressed in HEK 293, with >97% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
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Key facts
- Purity
- >97% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
M D R G V L P L P I T L L F V I Y S F V P T T G L A E R V G C D L Q P V D P T R G E V T F T T S Q V S E G C V A Q A A N A V R E V H V L F L D F P G M L S H L E L T L Q A S K Q N G T E T Q E V F L V L V S N K N V F V K F Q A P E I P L H L A Y D S S L V I F Q G Q P R V N I T V L P S L T S R K Q I L D W A A T K G A I T S I A A L D D P Q S I V L Q L G Q D P K A P F L C L P E A H K D M G A T L E W Q P R A Q T P V Q S C R L E G V S G H K E A Y I L R I L P G S E A G P R T V T V M M E L S C T S G D A I L I L H G P P Y V S W F I D I N H S M Q I L T T G E Y S V K I F P G S K V K G V E L P D T P Q G L I A E A R K L N A S I V T S F V E L P L V S N V S L R A S S C G G V F Q T T P A P V V T T P P K D T C S P V L L M S L I Q P K C G N Q V M T L A L N K K H V Q T L Q C T I T G L T F W D S S C Q A E D T D D H L V L S S A Y S S C G M K V T A H V V S N E V I I S F P S G S P P L R K K V Q C I D M D S L S F Q L G L Y L S P H F L Q A S N T I E L G Q Q A F V Q V S V S P L T S E V T V Q L D S C H L D L G P E G D M V E L I Q S R T A K G S C V T L L S P S P E G D P R F S F L L R V Y M V P T P T A G T L S C N L A L R P S T L S Q E V Y K T V S M R L N I V S P D L S G K
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Vascular endothelium glycoprotein that plays an important role in the regulation of angiogenesis (PubMed:10625534). Required for normal structure and integrity of adult vasculature (By similarity). Regulates the migration of vascular endothelial cells (PubMed:17540773). Required for normal extraembryonic angiogenesis and for embryonic heart development (PubMed:10625534). May regulate endothelial cell shape changes in response to blood flow, which drive vascular remodeling and establishment of normal vascular morphology during angiogenesis (PubMed:28530658). May play a role in the binding of endothelial cells to integrins. Acts as a TGF-beta coreceptor and is involved in the TGF-beta/BMP signaling cascade that ultimately leads to the activation of SMAD transcription factors (PubMed:23300529). Required for GDF2/BMP9 signaling through SMAD1 in endothelial cells and modulates TGFB1 signaling through SMAD3 (By similarity).
Alternative names
CD105, Edg, Eng, Endoglin, Cell surface MJ7/18 antigen
Recommended products
Recombinant mouse CD105 protein (His tag) is a Mouse Fragment protein, in the 1 to 580 aa range, expressed in HEK 293, with >97% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >97% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- Measured by its ability to inhibit BMP9-induced alkaline phosphatase production by MC3T3E1 mouse chondrogenic cells.
The ED50 for this effect is typically 25-100 ng/mL in the presence of 2 ng/mL of recombiant human BMP9.
- Accession
- Q63961-1
- Animal free
- No
- Species
- Mouse
- Concentration
- Storage buffer
pH: 7.4
Constituents: 100% PBS
Sequence info
Amino acid sequence
- M D R G V L P L P I T L L F V I Y S F V P T T G L A E R V G C D L Q P V D P T R G E V T F T T S Q V S E G C V A Q A A N A V R E V H V L F L D F P G M L S H L E L T L Q A S K Q N G T E T Q E V F L V L V S N K N V F V K F Q A P E I P L H L A Y D S S L V I F Q G Q P R V N I T V L P S L T S R K Q I L D W A A T K G A I T S I A A L D D P Q S I V L Q L G Q D P K A P F L C L P E A H K D M G A T L E W Q P R A Q T P V Q S C R L E G V S G H K E A Y I L R I L P G S E A G P R T V T V M M E L S C T S G D A I L I L H G P P Y V S W F I D I N H S M Q I L T T G E Y S V K I F P G S K V K G V E L P D T P Q G L I A E A R K L N A S I V T S F V E L P L V S N V S L R A S S C G G V F Q T T P A P V V T T P P K D T C S P V L L M S L I Q P K C G N Q V M T L A L N K K H V Q T L Q C T I T G L T F W D S S C Q A E D T D D H L V L S S A Y S S C G M K V T A H V V S N E V I I S F P S G S P P L R K K V Q C I D M D S L S F Q L G L Y L S P H F L Q A S N T I E L G Q Q A F V Q V S V S P L T S E V T V Q L D S C H L D L G P E G D M V E L I Q S R T A K G S C V T L L S P S P E G D P R F S F L L R V Y M V P T P T A G T L S C N L A L R P S T L S Q E V Y K T V S M R L N I V S P D L S G K
- Accession
- Q63961
- Protein length
- Fragment
- Predicted molecular weight
- 61.2 kDa
- Amino acids
- 1 to 580
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Lyophilized
General info
- Function
Vascular endothelium glycoprotein that plays an important role in the regulation of angiogenesis (PubMed:10625534). Required for normal structure and integrity of adult vasculature (By similarity). Regulates the migration of vascular endothelial cells (PubMed:17540773). Required for normal extraembryonic angiogenesis and for embryonic heart development (PubMed:10625534). May regulate endothelial cell shape changes in response to blood flow, which drive vascular remodeling and establishment of normal vascular morphology during angiogenesis (PubMed:28530658). May play a role in the binding of endothelial cells to integrins. Acts as a TGF-beta coreceptor and is involved in the TGF-beta/BMP signaling cascade that ultimately leads to the activation of SMAD transcription factors (PubMed:23300529). Required for GDF2/BMP9 signaling through SMAD1 in endothelial cells and modulates TGFB1 signaling through SMAD3 (By similarity).
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD105 also known as endoglin or the CD105 marker is a transmembrane glycoprotein with a molecular weight of approximately 180 kDa. It is a component of the TGF-beta receptor complex and exists in endothelial cells where it is abundantly expressed. Expression of CD105 is higher in proliferating cells particularly in the vasculature. You can also find it in tissues involved in the formation and remodeling of blood vessels such as during angiogenesis.
- Biological function summary
Endoglin functions in the regulation of angiogenesis and vascular remodeling. It plays a significant role in mediating cellular responses to TGF-beta signaling influencing endothelial cell proliferation and migration. While not part of a larger structural complex endoglin interacts with receptors and signaling molecules important for vascular development and repair processes. This involvement aids in maintaining endothelial integrity and function under various physiological conditions.
- Pathways
CD105 participates in the TGF-beta signaling and angiogenesis pathways. In these pathways it acts in conjunction with other proteins like TGF-beta receptors which play roles in cell differentiation proliferation and apoptosis. The interaction between CD105 and TGF-beta signaling regulates numerous cellular mechanisms impacting angiogenesis and cellular responses to environmental changes.
- Associated diseases and disorders
CD105 has links to hereditary hemorrhagic telangiectasia (HHT) and certain cancers. In HHT mutations in the endoglin gene alter vascular structure leading to the formation of abnormal blood vessels. Oncologically overexpression of CD105 is present in tumor angiogenesis aiding in the progression of certain cancers. Other proteins like VEGF and TGF-beta closely interact with endoglin influencing disease progression and presenting potential targets for therapeutic intervention.
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2 product images
Functional Studies - Recombinant mouse CD105 protein (His tag) (ab276838)
Measured by its ability to inhibit BMP9-induced alkaline phosphatase production by MC3T3E1 mouse chondrogenic cells.
The ED50 for this effect is typically 25-100 ng/mL in the presence of 2 ng/mL of recombiant human BMP9.
SDS-PAGE - Recombinant mouse CD105 protein (His tag) (ab276838)
SDS-PAGE analysis of ab276838
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