Recombinant mouse CD134 / OX40L receptor protein (Active) is a Mouse Fragment protein, in the 20 to 211 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
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Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
V T A R R L N C V K H T Y P S G H K C C R E C Q P G H G M V S R C D H T R D T L C H P C E T G F Y N E A V N Y D T C K Q C T Q C N H R S G S E L K Q N C T P T Q D T V C R C R P G T Q P R Q D S G Y K L G V D C V P C P P G H F S P G N N Q A C K P W T N C T L S G K Q T R H P A S D S L D A V C E D R S L L A T L L W E T Q R P T F R P T T V Q S T T V W P R T S E L P S P P T L V T P E G P
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Receptor for TNFSF4/OX40L/GP34. Is a costimulatory molecule implicated in long-term T-cell immunity (By similarity).
Alternative names
CD134, Ox40, Txgp1, Tnfrsf4, Tumor necrosis factor receptor superfamily member 4, OX40 antigen, OX40L receptor
Recommended products
Recombinant mouse CD134 / OX40L receptor protein (Active) is a Mouse Fragment protein, in the 20 to 211 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- Measured by its binding ability in a functional ELISA. Immobilized ab221028 at 2 μg/mL (100 μl/well) can bind mouse CD134 / OX40 Ligand, Fc Tag with a linear range of 2-30 ng/mL.
- Accession
- P47741-1
- Animal free
- No
- Species
- Mouse
- Reconstitution
- Reconstitute with sterile deionized water to a concentration of 400 µg/ml.
- Concentration
- Storage buffer
pH: 7.4
Constituents: 95% PBS, 5% Trehalose
Sequence info
Amino acid sequence
- V T A R R L N C V K H T Y P S G H K C C R E C Q P G H G M V S R C D H T R D T L C H P C E T G F Y N E A V N Y D T C K Q C T Q C N H R S G S E L K Q N C T P T Q D T V C R C R P G T Q P R Q D S G Y K L G V D C V P C P P G H F S P G N N Q A C K P W T N C T L S G K Q T R H P A S D S L D A V C E D R S L L A T L L W E T Q R P T F R P T T V Q S T T V W P R T S E L P S P P T L V T P E G P
- Accession
- P47741
- Protein length
- Fragment
- Predicted molecular weight
- 23.2 kDa
- Amino acids
- 20 to 211
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Lyophilized
General info
- Function
Receptor for TNFSF4/OX40L/GP34. Is a costimulatory molecule implicated in long-term T-cell immunity (By similarity).
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- Up to 12 months
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -80°C
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD134 also known as OX40 or TNFRSF4 is a receptor that plays an essential role in immune response regulation. This receptor is part of the tumor necrosis factor receptor (TNFR) superfamily and has a molecular mass of approximately 50 kDa. CD134 is expressed on activated T cells particularly CD4+ T cells and is present on various other immune cells under specific conditions. The receptor binds to the OX40L its ligand which is expressed on antigen-presenting cells such as dendritic cells and B cells.
- Biological function summary
CD134 influences T cell proliferation survival and memory formation being an important component of the immune system's adaptive response. OX40L engagement with CD134 enhances the activation and survival of T cells promoting long-term immunity. This interaction does not form part of a larger complex but functions as a direct signaling mechanism that augments T cell receptor (TCR) signaling leading to increased cytokine production and sustained immune responses essential for controlling infections and cancer.
- Pathways
CD134 signaling intersects with the nuclear factor-kappa B (NF-κB) and phosphoinositide 3-kinase (PI3K) pathways. These pathways play significant roles in regulating immune responses and cell survival. Through the NF-κB pathway CD134 influences gene transcription related to immune activation. It also connects with other immune regulatory proteins such as CD28 which further potentiates T cell responses and enhances effector functions.
- Associated diseases and disorders
CD134 has connections to autoimmune diseases and cancers. Overactivity of the CD134-OX40L pathway can lead to exacerbated autoimmune conditions such as lupus. This receptor has also been implicated in tumor immunity where its activation enhances the anti-tumor response of T cells. CD134 interacts with CTLA-4 another immune checkpoint protein playing a role in balancing immune activation and regulation within these disease contexts.
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4 product images
Functional Studies - Recombinant mouse CD134 / OX40L receptor protein (Active) (ab221028)
Immobilized Mouse OX40 Ligand (Fc Tag) at 2 µg/mL (100 µL/well) can bind Mouse OX40 (His Tag) with a linear range of 3.9-31.3 ng/mL (QC tested).
SDS-PAGE - Recombinant mouse CD134 / OX40L receptor protein (Active) (ab221028)
Mouse OX40 (His Tag) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
Functional Studies - Recombinant mouse CD134 / OX40L receptor protein (Active) (ab221028)
Immobilized ab221028 at 2 μg/mL (100 μl/well) can bind mouse CD134 / OX40 Ligand, Fc Tag with a linear range of 2-30 ng/mL.
SDS-PAGE - Recombinant mouse CD134 / OX40L receptor protein (Active) (ab221028)
SDS-PAGE analysis of reduced ab221028 stained overnight with Coomassie Blue. DTT-reduced protein migrates as 45 kDa in SDS-PAGE due to glycosylation.
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