Recombinant Mouse CD27 protein (Tagged) is a Mouse Fragment protein, in the 21 to 182 aa range, expressed in HEK 293, with >=90% purity and suitable for SDS-PAGE, WB.
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Key facts
- Purity
- >=90% SDS-PAGE
- Expression system
- HEK 293 cells
- Tags
- Avi tag C-Terminus Fc tag C-Terminus
- Applications
- SDS-PAGE, WB
- Biologically active
- No
Amino acid sequence
T L A P N S C P D K H Y W T G G G L C C R M C E P G T F F V K D C E Q D R T A A Q C D P C I P G T S F S P D Y H T R P H C E S C R H C N S G F L I R N C T V T A N A E C S C S K N W Q C R D Q E C T E C D P P L N P A L T R Q P S E T P S P Q P P P T H L P H G T E K P S W P L H R Q L P N S T V Y S Q R S S H R P L C S S D C I R
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Costimulatory immune-checkpoint receptor expressed at the surface of T-cells, NK-cells and B-cells which binds to and is activated by its ligand CD70/CD27L expressed by B-cells. The CD70-CD27 signaling pathway mediates antigen-specific T-cell activation and expansion which in turn provides immune surveillance of B-cells (PubMed:11062504). Mechanistically, CD70 ligation activates the TRAF2-PTPN6 axis that subsequently inhibits LCK phosphorylation to promote phenotypic and transcriptional adaptations of T-cell memory (By similarity). In addition, activation by CD70 on early progenitor cells provides a negative feedback signal to leukocyte differentiation during immune activation and thus modulates hematopoiesis (PubMed:15723067). Negatively regulates the function of Th2 lymphocytes in the adipose tissue (PubMed:38386557).
Alternative names
CD27, Tnfrsf7, Cd27, CD27 antigen, CD27L receptor, T-cell activation antigen CD27, Tumor necrosis factor receptor superfamily member 7
Recommended products
Recombinant Mouse CD27 protein (Tagged) is a Mouse Fragment protein, in the 21 to 182 aa range, expressed in HEK 293, with >=90% purity and suitable for SDS-PAGE, WB.
Key facts
- Purity
- >=90% SDS-PAGE
- Expression system
- HEK 293 cells
- Tags
- Avi tag C-Terminus Fc tag C-Terminus
- Applications
- SDS-PAGE, WB
- Biologically active
- No
- Accession
- P41272-1
- Animal free
- No
- Species
- Mouse
- Concentration
- Storage buffer
pH: 7.4
Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.13% Sodium phosphate, 0.02% Potassium chloride
Sequence info
Amino acid sequence
- T L A P N S C P D K H Y W T G G G L C C R M C E P G T F F V K D C E Q D R T A A Q C D P C I P G T S F S P D Y H T R P H C E S C R H C N S G F L I R N C T V T A N A E C S C S K N W Q C R D Q E C T E C D P P L N P A L T R Q P S E T P S P Q P P P T H L P H G T E K P S W P L H R Q L P N S T V Y S Q R S S H R P L C S S D C I R
- Accession
- P41272
- Protein length
- Fragment
- Predicted molecular weight
- 49 kDa
- Amino acids
- 21 to 182
- Nature
- Recombinant
- Tags
- Avi tag C-Terminus, Fc tag C-Terminus
Specifications
- Form
- Liquid
General info
- Function
Costimulatory immune-checkpoint receptor expressed at the surface of T-cells, NK-cells and B-cells which binds to and is activated by its ligand CD70/CD27L expressed by B-cells. The CD70-CD27 signaling pathway mediates antigen-specific T-cell activation and expansion which in turn provides immune surveillance of B-cells (PubMed:11062504). Mechanistically, CD70 ligation activates the TRAF2-PTPN6 axis that subsequently inhibits LCK phosphorylation to promote phenotypic and transcriptional adaptations of T-cell memory (By similarity). In addition, activation by CD70 on early progenitor cells provides a negative feedback signal to leukocyte differentiation during immune activation and thus modulates hematopoiesis (PubMed:15723067). Negatively regulates the function of Th2 lymphocytes in the adipose tissue (PubMed:38386557).
- Post-translational modifications
Phosphorylated.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD27 also known as Tumor Necrosis Factor Receptor Superfamily Member 7 (TNFRSF7) is a protein chiefly involved in T cell activation and proliferation. It belongs to the TNF-receptor superfamily and weighs approximately 50 kDa. CD27 is expressed mainly on T lymphocytes and to a lesser extent on B cells and natural killer cells. Researchers often study CD27 and its ligand CD70 to understand immune responses. Tools such as anti-CD27 percp 7h21 monoclonal antibodies and APC-conjugated forms help detect this protein in various assays.
- Biological function summary
One finds CD27 at the heart of cellular immune responses. It does not function alone; instead it typically forms part of a receptor-ligand complex with CD70 which influences T cell and B cell activities. This interaction promotes cell survival and plays a role in the activation of NF-kB and MAPK8/JNK signaling pathways which are essential for immune response regulation. These pathways ensure that the body's immune cells can efficiently respond to pathogens.
- Pathways
CD27 actively integrates into the NF-kB and mTOR signaling pathways which are important for regulating immune and inflammatory responses. Through these pathways CD27 works closely with proteins like TRAF2 and TRAF3 which are adapter molecules that transmit signals from the receptor. These signaling pathways provide a framework for understanding how CD27 and associated proteins work together to control immune cell behavior facilitating coordinated immune defense mechanisms.
- Associated diseases and disorders
CD27 has significant implications in autoimmune diseases and cancer. For example aberrant activation of CD27 may contribute to conditions like lymphoma and autoimmune diseases such as lupus. The CD70-CD27 signaling axis when dysregulated can lead to uncontrolled cell proliferation or apoptosis linking it to these pathologies. Understanding the interplay between CD27 and proteins like Bcl-2 which influences apoptosis helps pave the way for therapeutic strategies targeting these diseases.
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1 product image
SDS-PAGE - Recombinant Mouse CD27 protein (Tagged) (ab271419)
SDS-PAGE analysis of ab271419.
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