Recombinant Mouse CD28 Protein is a Mouse Fragment protein, in the 20 to 149 aa range, expressed in HEK 293, with >=95% purity and <= 0.005 EU/µg endotoxin level.
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Key facts
- Purity
>=95% SDS-PAGE
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
HEK 293 cells
- Tags
Tag free
- Biologically active
No
Amino acid sequence
N K I L V K Q S P L L V V D S N E V S L S C R Y S Y N L L A K E F R A S L Y K G V N S D V E V C V G N G N F T Y Q P Q F R S N A E F N C D G D F D N E T V T F R L W N L H V N H T D I Y F C K I E F M Y P P P Y L D N E R S N G T I I H I K E K H L C H T Q S S P K
Target data
Function
Receptor that plays a role in T-cell activation, proliferation, survival and the maintenance of immune homeostasis (PubMed:7688139). Functions not only as an amplifier of TCR signals but delivers unique signals that control intracellular biochemical events that alter the gene expression program of T-cells. Stimulation upon engagement of its cognate ligands CD80 or CD86 increases proliferation and expression of various cytokines in particular IL2 production in both CD4(+) and CD8(+) T-cell subsets. Mechanistically, ligation induces recruitment of protein kinase C-theta/PRKCQ and GRB2 leading to NF-kappa-B activation via both PI3K/Akt-dependent and -independent pathways. In conjunction with TCR/CD3 ligation and CD40L costimulation, enhances the production of IL4 and IL10 in T-cells.
Alternative names
CD28, T-cell-specific surface glycoprotein CD28, Cd28
Recommended products
Recombinant Mouse CD28 Protein is a Mouse Fragment protein, in the 20 to 149 aa range, expressed in HEK 293, with >=95% purity and <= 0.005 EU/µg endotoxin level.
Key facts
- Purity
>=95% SDS-PAGE
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
HEK 293 cells
- Tags
Tag free
- Biologically active
No
- Accession
- P31041-1
- Animal free
No
- Species
Mouse
- Reconstitution
Reconstitute in PBS
- Concentration
- Storage buffer
pH: 7.4
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic
Sequence info
Amino acid sequence
N K I L V K Q S P L L V V D S N E V S L S C R Y S Y N L L A K E F R A S L Y K G V N S D V E V C V G N G N F T Y Q P Q F R S N A E F N C D G D F D N E T V T F R L W N L H V N H T D I Y F C K I E F M Y P P P Y L D N E R S N G T I I H I K E K H L C H T Q S S P K
- Accession
- P31041
- Protein length
Fragment
- Amino acids
20 to 149
- Nature
Recombinant
Specifications
- Form
Lyophilized
General info
- Function
Receptor that plays a role in T-cell activation, proliferation, survival and the maintenance of immune homeostasis (PubMed:7688139). Functions not only as an amplifier of TCR signals but delivers unique signals that control intracellular biochemical events that alter the gene expression program of T-cells. Stimulation upon engagement of its cognate ligands CD80 or CD86 increases proliferation and expression of various cytokines in particular IL2 production in both CD4(+) and CD8(+) T-cell subsets. Mechanistically, ligation induces recruitment of protein kinase C-theta/PRKCQ and GRB2 leading to NF-kappa-B activation via both PI3K/Akt-dependent and -independent pathways. In conjunction with TCR/CD3 ligation and CD40L costimulation, enhances the production of IL4 and IL10 in T-cells.
- Post-translational modifications
Phosphorylated by LCK (PubMed:27460989). Dephosphorylated by PTPN11 (By similarity).
Storage
- Shipped at conditions
Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
Ambient
- Appropriate long-term storage conditions
Ambient
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD28 also known as T-cell-specific surface glycoprotein CD28 or TP44 plays an important role as a co-stimulatory receptor on T cells. This glycoprotein has a molecular mass of about 44 kDa and is located mainly on the cell surface of T cells. CD28 expression also occurs on thymocytes and some B cells. It forms part of the immunoglobulin superfamily featuring in significant antibody interactions. Researchers often use anti-CD28 antibodies for various immunological assays including CD28 ELISA to study its function.
- Biological function summary
CD28 co-stimulates T cells after antigen presentation. It enhances T cell proliferation survival and cytokine production. CD28 functions alongside the T cell receptor (TCR) complex mediating signals that promote full T cell activation. This interaction amplifies the immune response forming an important axis of immune system communication. CD28 interacts with its ligands B7-1 (CD80) and B7-2 (CD86) found on antigen-presenting cells (APCs) illustrating its role in immune system dynamics.
- Pathways
CD28 is integral to the PI3K/Akt and MAPK/ERK signaling pathways. These pathways conduct signals critical for cellular growth and survival linking CD28 to downstream transcription factors. The interaction with another protein CTLA-4 acts as a regulatory balance for CD28 signaling preventing over-activation of T cells. This balance influences immune homeostasis and tolerance providing a framework for understanding costimulatory and inhibitory signaling.
- Associated diseases and disorders
CD28's role becomes evident in autoimmune diseases and transplant rejection. Abnormal CD28 signaling links to rheumatoid arthritis where inappropriate T cell activation occurs. Similarly CD28 activity contributes to organ transplant rejection by stimulating T cell-mediated responses against transplanted tissues. CTLA-4 through regulatory feedback can modify these effects providing therapeutic target strategies for managing such disorders. Efforts to modulate CD28 activity involve developing therapeutic agents such as anti-CD28 monoclonal antibodies.
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SDS-PAGE - Recombinant Mouse CD28 Protein (ab290088)
SDS-PAGE analysis of ab290088
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