Recombinant Mouse CD3 epsilon + CD3G protein (Tagged) is a Mouse Fragment protein, in the 1 to 108 aa range, expressed in HEK 293, with =95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Images
Key facts
- Purity
- =95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Fc tag C-Terminus His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Sequence
This product is comprised of multiple sequences see
Amino acid sequence 1
M R W N T F W G I L C L S L L A V G T C Q D D A E N I E Y K V S I S G T S V E L T C P L D S D E N L K W E K N G Q E L P Q K H D K H L V L Q D F S E V E D S G Y Y V C Y T P A S N K N T Y L Y L K A R V C E Y C V E V D
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell development (PubMed:19956738, PubMed:24899501). Participates also in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region. In addition to its role as a TCR coreceptor, it serves as a receptor for ITPRIPL1. Ligand recognition inhibits T-cell activation by promoting interaction with NCK1, which prevents CD3E-ZAP70 interaction and blocks the ERK-NFkB signaling cascade and calcium influx (By similarity).
Additional Targets
T-cell surface glycoprotein CD3 gamma chain
Alternative names
CD3e, T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, Cd3e
Recommended products
Recombinant Mouse CD3 epsilon + CD3G protein (Tagged) is a Mouse Fragment protein, in the 1 to 108 aa range, expressed in HEK 293, with =95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Key facts
- Purity
- =95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Fc tag C-Terminus His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P22646-1
- Animal free
- No
- Species
- Mouse
- Concentration
- Storage buffer
pH: 7.4
Constituents: 100% PBS
Sequence info
Amino acid sequence 1
- M R W N T F W G I L C L S L L A V G T C Q D D A E N I E Y K V S I S G T S V E L T C P L D S D E N L K W E K N G Q E L P Q K H D K H L V L Q D F S E V E D S G Y Y V C Y T P A S N K N T Y L Y L K A R V C E Y C V E V D
- Accession
- P22646
- Protein length
- Fragment
- Predicted molecular weight
- 38.1 kDa
- Amino acids
- 1 to 108
- Nature
- Recombinant
- Tags
- Fc tag C-Terminus, His tag C-Terminus
Amino acid sequence 2
- Accession
- P11942
- Protein length
- Fragment
- Predicted molecular weight
- 37.3 kDa
- Nature
- Recombinant
- Tags
- Fc tag C-Terminus
Specifications
- Form
- Lyophilized
General info
- Function
Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell development (PubMed:19956738, PubMed:24899501). Participates also in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region. In addition to its role as a TCR coreceptor, it serves as a receptor for ITPRIPL1. Ligand recognition inhibits T-cell activation by promoting interaction with NCK1, which prevents CD3E-ZAP70 interaction and blocks the ERK-NFkB signaling cascade and calcium influx (By similarity).
- Post-translational modifications
Phosphorylated on Tyr residues after T-cell receptor triggering by LCK in association with CD4/CD8.
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
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Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
SDS-PAGE - Recombinant Mouse CD3 epsilon + CD3G protein (Tagged) (ab277033)
SDS-PAGE analysis of ab277033
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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